Galliform aveparvovirus 1 (isolate Chicken/Hungary/ABU-P1/-/-) (ChPV)
Average proteome isoelectric point is 5.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3X6W9|D3X6W9_CHPV1 VP1 OS=Galliform aveparvovirus 1 (isolate Chicken/Hungary/ABU-P1/-/-) OX=717641 GN=VP1 PE=3 SV=1
MM1 pKa = 7.54 CWYY4 pKa = 9.58 NANITQIQTVEE15 pKa = 4.34 GDD17 pKa = 3.29 PCTNPDD23 pKa = 3.7 VFHH26 pKa = 7.01 EE27 pKa = 4.94 LYY29 pKa = 10.8 FPIKK33 pKa = 9.05 QHH35 pKa = 6.94 AMDD38 pKa = 4.3 ARR40 pKa = 11.84 MLKK43 pKa = 9.56 MKK45 pKa = 10.35 IEE47 pKa = 4.43 DD48 pKa = 4.56 KK49 pKa = 10.43 IQKK52 pKa = 10.38 GDD54 pKa = 3.54 FTQDD58 pKa = 2.11 IHH60 pKa = 9.4 LDD62 pKa = 3.55 MTLTQCIQTALSDD75 pKa = 4.11 DD76 pKa = 4.07 PAGIWPCVDD85 pKa = 3.88 RR86 pKa = 11.84 LANYY90 pKa = 10.1 CNVILASIGTQQ101 pKa = 2.95
Molecular weight: 11.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.312
IPC2_protein 4.635
IPC_protein 4.507
Toseland 4.329
ProMoST 4.685
Dawson 4.52
Bjellqvist 4.673
Wikipedia 4.469
Rodwell 4.368
Grimsley 4.253
Solomon 4.52
Lehninger 4.469
Nozaki 4.647
DTASelect 4.914
Thurlkill 4.393
EMBOSS 4.495
Sillero 4.66
Patrickios 2.053
IPC_peptide 4.507
IPC2_peptide 4.635
IPC2.peptide.svr19 4.57
Protein with the highest isoelectric point:
>tr|D3X6X0|D3X6X0_CHPV1 VP2 OS=Galliform aveparvovirus 1 (isolate Chicken/Hungary/ABU-P1/-/-) OX=717641 GN=VP2 PE=3 SV=1
MM1 pKa = 7.73 KK2 pKa = 10.34 YY3 pKa = 9.62 KK4 pKa = 10.54 APKK7 pKa = 9.99 GYY9 pKa = 10.38 VPSLPTTDD17 pKa = 3.26 EE18 pKa = 3.84 EE19 pKa = 5.11 AYY21 pKa = 10.57 HH22 pKa = 5.48 HH23 pKa = 5.6 TRR25 pKa = 11.84 WWLDD29 pKa = 3.09 HH30 pKa = 6.06 VEE32 pKa = 4.21 NYY34 pKa = 9.84 GKK36 pKa = 10.32 NKK38 pKa = 10.47 AIGRR42 pKa = 11.84 GKK44 pKa = 10.37 GKK46 pKa = 10.08 ARR48 pKa = 11.84 KK49 pKa = 8.92 EE50 pKa = 3.58 LTPQQKK56 pKa = 10.52 AEE58 pKa = 4.06 RR59 pKa = 11.84 KK60 pKa = 9.73 RR61 pKa = 11.84 FFITQAQKK69 pKa = 11.0 NKK71 pKa = 9.36 KK72 pKa = 8.18 PNYY75 pKa = 8.63 GIPSSSQHH83 pKa = 6.38 KK84 pKa = 10.15 EE85 pKa = 3.51 FFKK88 pKa = 10.88 NHH90 pKa = 5.37 QGAQGGKK97 pKa = 9.06 AKK99 pKa = 10.48 QKK101 pKa = 9.22 TKK103 pKa = 10.32 HH104 pKa = 6.26 IEE106 pKa = 3.78 NQAPQRR112 pKa = 11.84 QEE114 pKa = 4.02 DD115 pKa = 4.5 PEE117 pKa = 4.44 EE118 pKa = 4.84 GPAPKK123 pKa = 9.94 QPRR126 pKa = 11.84 VEE128 pKa = 4.13 EE129 pKa = 3.82 NPYY132 pKa = 9.72 NQEE135 pKa = 3.76 EE136 pKa = 4.7 LEE138 pKa = 4.14 EE139 pKa = 4.18 MMADD143 pKa = 3.59 EE144 pKa = 5.22 NEE146 pKa = 4.24 PDD148 pKa = 3.26 QSGIPMEE155 pKa = 5.07 LSDD158 pKa = 5.16 GPGTVGGGGGGGIGHH173 pKa = 6.08 STGNWNCDD181 pKa = 3.88 TIWAEE186 pKa = 4.35 NTCKK190 pKa = 10.12 TYY192 pKa = 11.09 ASRR195 pKa = 11.84 HH196 pKa = 5.21 CVCLMRR202 pKa = 11.84 DD203 pKa = 3.08 MDD205 pKa = 3.74 QYY207 pKa = 11.7 KK208 pKa = 10.82 AIGNANGQHH217 pKa = 6.56 GLDD220 pKa = 4.19 TEE222 pKa = 4.3 NQTQYY227 pKa = 11.33 FGFSTPWNYY236 pKa = 9.77 IDD238 pKa = 4.04 FNQYY242 pKa = 9.58 SVHH245 pKa = 6.49 FSPRR249 pKa = 11.84 DD250 pKa = 3.35 WQQLVNNYY258 pKa = 9.49 SRR260 pKa = 11.84 WRR262 pKa = 11.84 PRR264 pKa = 11.84 AVHH267 pKa = 6.1 VKK269 pKa = 9.8 IFNLQVIQKK278 pKa = 7.67 TVTDD282 pKa = 3.47 SGTQYY287 pKa = 11.55 SNDD290 pKa = 3.3 LTGTIQIFADD300 pKa = 3.29 QEE302 pKa = 3.86 GRR304 pKa = 11.84 YY305 pKa = 8.96 PRR307 pKa = 11.84 LLYY310 pKa = 10.11 PNQTTLMGPFPSNIYY325 pKa = 9.89 YY326 pKa = 10.39 LPQYY330 pKa = 11.35 AYY332 pKa = 6.86 MTSMFNSGGPTVSSWWGFPTRR353 pKa = 11.84 EE354 pKa = 3.94 SAFYY358 pKa = 10.8 CLDD361 pKa = 3.62 EE362 pKa = 5.5 SPSQMLRR369 pKa = 11.84 TGNEE373 pKa = 3.5 WEE375 pKa = 4.34 TTFIFPSSTQWCSNKK390 pKa = 9.53 ISTVSITQRR399 pKa = 11.84 QNPLYY404 pKa = 9.56 DD405 pKa = 3.09 TWNVNGRR412 pKa = 11.84 GDD414 pKa = 3.5 DD415 pKa = 3.43 AKK417 pKa = 10.82 RR418 pKa = 11.84 GNFCTWRR425 pKa = 11.84 SPWYY429 pKa = 8.08 PGPYY433 pKa = 9.04 IYY435 pKa = 10.27 LTDD438 pKa = 3.69 TSAASQRR445 pKa = 11.84 LEE447 pKa = 3.71 NLANVAVGPDD457 pKa = 4.01 GMPLAPGMPQYY468 pKa = 10.59 RR469 pKa = 11.84 SEE471 pKa = 4.16 SDD473 pKa = 2.88 KK474 pKa = 11.71 DD475 pKa = 3.75 EE476 pKa = 4.33 YY477 pKa = 11.7 LHH479 pKa = 6.2 TFWVPKK485 pKa = 8.65 KK486 pKa = 10.66 VKK488 pKa = 9.85 MNEE491 pKa = 3.38 GDD493 pKa = 3.24 IRR495 pKa = 11.84 NAQIDD500 pKa = 3.9 VSTATKK506 pKa = 10.3 VQVDD510 pKa = 3.66 TSTLYY515 pKa = 8.65 NTCPRR520 pKa = 11.84 SGYY523 pKa = 9.47 MSTVSQGGAPAPIVWSGCVPGMIWDD548 pKa = 4.13 NRR550 pKa = 11.84 PATYY554 pKa = 10.12 FDD556 pKa = 5.85 PIWQQFPEE564 pKa = 4.05 TDD566 pKa = 3.39 EE567 pKa = 3.85 QFKK570 pKa = 10.7 IISQLGGIPVKK581 pKa = 10.2 EE582 pKa = 4.33 SPGHH586 pKa = 5.77 IFVKK590 pKa = 8.93 VTPKK594 pKa = 9.21 PTGAANGLIDD604 pKa = 4.38 EE605 pKa = 4.89 YY606 pKa = 11.14 CTFTCTVEE614 pKa = 4.23 IEE616 pKa = 4.34 WEE618 pKa = 4.11 LEE620 pKa = 3.66 PLNTHH625 pKa = 5.84 RR626 pKa = 11.84 WNMRR630 pKa = 11.84 SMISYY635 pKa = 7.73 EE636 pKa = 4.11 TTDD639 pKa = 3.03 AKK641 pKa = 10.71 AGRR644 pKa = 11.84 QIVDD648 pKa = 3.27 EE649 pKa = 4.36 NGQYY653 pKa = 9.53 QVNVNSADD661 pKa = 3.08 ITRR664 pKa = 11.84 LYY666 pKa = 6.64 MTKK669 pKa = 10.31 RR670 pKa = 11.84 APRR673 pKa = 11.84 TNN675 pKa = 3.16
Molecular weight: 76.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.98
IPC2_protein 6.046
IPC_protein 6.122
Toseland 6.402
ProMoST 6.491
Dawson 6.427
Bjellqvist 6.427
Wikipedia 6.44
Rodwell 6.427
Grimsley 6.517
Solomon 6.427
Lehninger 6.427
Nozaki 6.707
DTASelect 6.883
Thurlkill 6.898
EMBOSS 6.868
Sillero 6.81
Patrickios 3.935
IPC_peptide 6.453
IPC2_peptide 6.693
IPC2.peptide.svr19 6.707
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2006
101
694
501.5
56.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.334 ± 0.19
2.542 ± 0.434
5.234 ± 0.415
5.833 ± 0.663
3.838 ± 0.292
7.228 ± 0.521
2.044 ± 0.206
5.583 ± 0.407
5.135 ± 0.771
5.982 ± 1.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.891 ± 0.212
6.231 ± 0.138
6.78 ± 0.206
5.384 ± 0.683
4.187 ± 0.307
6.73 ± 0.429
7.428 ± 0.76
4.786 ± 0.171
2.742 ± 0.273
4.088 ± 0.556
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here