Chlamydiales bacterium SCGC AG-110-P3
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1225 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A212KVQ9|A0A212KVQ9_9CHLA Uncharacterized protein OS=Chlamydiales bacterium SCGC AG-110-P3 OX=1871323 GN=SCG7086_BH_00090 PE=4 SV=1
MM1 pKa = 7.16 YY2 pKa = 10.46 LLRR5 pKa = 11.84 VLILFIIGFSGSLLSVEE22 pKa = 4.36 AAADD26 pKa = 3.59 VGGQPASGFLLNKK39 pKa = 7.92 PTASGQGVVHH49 pKa = 6.79 GNMFPYY55 pKa = 9.92 TIYY58 pKa = 10.75 YY59 pKa = 10.31 DD60 pKa = 3.62 PAKK63 pKa = 10.33 WEE65 pKa = 4.12 MFAGNPGDD73 pKa = 3.48 EE74 pKa = 4.22 RR75 pKa = 11.84 EE76 pKa = 4.37 YY77 pKa = 10.87 TFSPVDD83 pKa = 3.61 GSQAQVWVEE92 pKa = 3.74 FRR94 pKa = 11.84 SEE96 pKa = 3.88 RR97 pKa = 11.84 VHH99 pKa = 6.15 YY100 pKa = 10.5 QEE102 pKa = 6.04 LPQNLIDD109 pKa = 4.56 EE110 pKa = 4.61 VTADD114 pKa = 3.99 GLSDD118 pKa = 3.55 VQIEE122 pKa = 4.37 LFEE125 pKa = 4.04 MLEE128 pKa = 4.37 VNGKK132 pKa = 9.45 YY133 pKa = 10.63 YY134 pKa = 10.35 IFIHH138 pKa = 5.26 MTADD142 pKa = 3.04 TGDD145 pKa = 3.72 DD146 pKa = 4.1 DD147 pKa = 6.08 KK148 pKa = 12.12 LMGYY152 pKa = 9.22 IFHH155 pKa = 7.04 YY156 pKa = 9.76 LTDD159 pKa = 3.69 AGMLRR164 pKa = 11.84 LAFVTLEE171 pKa = 4.24 SEE173 pKa = 4.8 GDD175 pKa = 3.48 VHH177 pKa = 7.62 DD178 pKa = 5.93 QDD180 pKa = 4.78 FQDD183 pKa = 4.55 LLFSTEE189 pKa = 4.03 LQDD192 pKa = 4.12
Molecular weight: 21.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.819
IPC2_protein 4.05
IPC_protein 4.024
Toseland 3.821
ProMoST 4.113
Dawson 3.999
Bjellqvist 4.177
Wikipedia 3.91
Rodwell 3.846
Grimsley 3.732
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.317
Thurlkill 3.859
EMBOSS 3.923
Sillero 4.139
Patrickios 1.939
IPC_peptide 3.986
IPC2_peptide 4.113
IPC2.peptide.svr19 4.036
Protein with the highest isoelectric point:
>tr|A0A212KWQ3|A0A212KWQ3_9CHLA Uncharacterized protein (Fragment) OS=Chlamydiales bacterium SCGC AG-110-P3 OX=1871323 GN=SCG7086_AK_00010 PE=4 SV=1
MM1 pKa = 7.7 CEE3 pKa = 3.83 LVEE6 pKa = 4.2 LRR8 pKa = 11.84 VVRR11 pKa = 11.84 LKK13 pKa = 10.53 RR14 pKa = 11.84 VRR16 pKa = 11.84 VGGAHH21 pKa = 7.06 LGEE24 pKa = 4.41 LPEE27 pKa = 4.11 RR28 pKa = 11.84 HH29 pKa = 5.46 WRR31 pKa = 11.84 HH32 pKa = 5.6 LSKK35 pKa = 10.73 EE36 pKa = 4.01 ATNCGTFRR44 pKa = 11.84 RR45 pKa = 11.84 GLKK48 pKa = 9.81 IGNCKK53 pKa = 10.17 NYY55 pKa = 10.0 RR56 pKa = 11.84 NRR58 pKa = 11.84 RR59 pKa = 11.84 GSFQGNTSGGRR70 pKa = 11.84 EE71 pKa = 3.98 HH72 pKa = 7.99 SKK74 pKa = 8.12 MTLLCEE80 pKa = 3.94 GHH82 pKa = 6.55 RR83 pKa = 11.84 LVFGVRR89 pKa = 11.84 KK90 pKa = 9.14 LAFAFFSSRR99 pKa = 11.84 LTGVPCGYY107 pKa = 10.55 LRR109 pKa = 11.84 ILKK112 pKa = 9.75 RR113 pKa = 11.84 DD114 pKa = 3.31 SNAFFLPQVRR124 pKa = 11.84 SLDD127 pKa = 3.82 GLEE130 pKa = 4.16 SSHH133 pKa = 7.23 LFFYY137 pKa = 11.04 LEE139 pKa = 3.98 QLTGPKK145 pKa = 10.14 GRR147 pKa = 11.84 QQAGYY152 pKa = 10.21
Molecular weight: 17.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.677
IPC_protein 10.555
Toseland 10.745
ProMoST 10.496
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 10.994
Grimsley 10.877
Solomon 10.965
Lehninger 10.935
Nozaki 10.745
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.716
IPC_peptide 10.979
IPC2_peptide 9.823
IPC2.peptide.svr19 8.538
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1225
0
1225
363987
30
3963
297.1
33.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.135 ± 0.074
1.327 ± 0.031
5.636 ± 0.053
6.663 ± 0.073
3.859 ± 0.047
6.824 ± 0.068
2.31 ± 0.038
6.255 ± 0.064
5.184 ± 0.09
10.275 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.339 ± 0.034
3.667 ± 0.053
4.194 ± 0.044
3.969 ± 0.06
5.808 ± 0.06
6.958 ± 0.073
5.805 ± 0.055
6.754 ± 0.065
1.174 ± 0.026
2.858 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here