Pseudoalteromonas phage H101

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Shandongvirus; Pseudoalteromonas virus H101

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4J256|A0A0H4J256_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage H101 OX=1654919 PE=4 SV=1
MM1 pKa = 7.46HH2 pKa = 7.75LVTNINGYY10 pKa = 9.36NMEE13 pKa = 4.27SLEE16 pKa = 4.24SIIYY20 pKa = 10.33SRR22 pKa = 11.84TGLNSVDD29 pKa = 4.08QLVDD33 pKa = 3.5DD34 pKa = 5.43LGIDD38 pKa = 3.58EE39 pKa = 4.7

Molecular weight: 4.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4INW5|A0A0H4INW5_9CAUD Adenine-specific methyltransferase OS=Pseudoalteromonas phage H101 OX=1654919 PE=4 SV=1
MM1 pKa = 6.12ITARR5 pKa = 11.84IIFKK9 pKa = 9.9RR10 pKa = 11.84DD11 pKa = 2.86QSGYY15 pKa = 8.9IKK17 pKa = 10.47PSITRR22 pKa = 11.84PFSMYY27 pKa = 10.84SDD29 pKa = 2.79IKK31 pKa = 10.23TFIRR35 pKa = 11.84ANKK38 pKa = 8.54DD39 pKa = 3.1TVLKK43 pKa = 10.93VVDD46 pKa = 4.3RR47 pKa = 11.84YY48 pKa = 10.39GVRR51 pKa = 11.84II52 pKa = 3.73

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

228

0

228

37698

37

917

165.3

18.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.364 ± 0.228

1.483 ± 0.109

6.756 ± 0.115

7.671 ± 0.217

4.09 ± 0.112

6.6 ± 0.219

1.804 ± 0.095

5.984 ± 0.132

7.605 ± 0.282

8.435 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.109

5.48 ± 0.157

2.881 ± 0.104

3.531 ± 0.119

3.841 ± 0.106

6.783 ± 0.191

5.968 ± 0.209

6.984 ± 0.148

1.454 ± 0.064

4.87 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski