Mycobacterium phage Luchador

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6YQB9|A0A0F6YQB9_9CAUD Lysin B OS=Mycobacterium phage Luchador OX=1647300 GN=11 PE=4 SV=1
MM1 pKa = 7.29TKK3 pKa = 10.4APSWAMTALCAVWAFGLTMALGEE26 pKa = 4.68LYY28 pKa = 9.33TARR31 pKa = 11.84AHH33 pKa = 7.12AEE35 pKa = 3.97PEE37 pKa = 3.85QVMTVDD43 pKa = 3.53GVDD46 pKa = 3.47YY47 pKa = 9.14PVCVEE52 pKa = 4.74EE53 pKa = 5.36DD54 pKa = 4.0CSDD57 pKa = 3.34QPGQIGIWIDD67 pKa = 3.4QDD69 pKa = 3.94TGNQWLSLGEE79 pKa = 3.54EE80 pKa = 4.42SYY82 pKa = 10.84RR83 pKa = 11.84IEE85 pKa = 4.01RR86 pKa = 3.85

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6YQ27|A0A0F6YQ27_9CAUD Head-to-tail stopper OS=Mycobacterium phage Luchador OX=1647300 GN=21 PE=4 SV=1
MM1 pKa = 7.08TWASSRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 9.77DD11 pKa = 4.74LPPDD15 pKa = 3.3WEE17 pKa = 4.53TIRR20 pKa = 11.84LSVLRR25 pKa = 11.84DD26 pKa = 3.39ADD28 pKa = 4.36WICEE32 pKa = 3.75LRR34 pKa = 11.84MEE36 pKa = 4.54DD37 pKa = 3.51VCLGTASEE45 pKa = 4.2VDD47 pKa = 3.76HH48 pKa = 6.9INRR51 pKa = 11.84GNDD54 pKa = 3.08HH55 pKa = 6.35SRR57 pKa = 11.84SNLRR61 pKa = 11.84AVCHH65 pKa = 6.12RR66 pKa = 11.84CHH68 pKa = 6.52AKK70 pKa = 10.26KK71 pKa = 10.66SSAEE75 pKa = 3.7GNARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.73LRR84 pKa = 11.84AQRR87 pKa = 11.84KK88 pKa = 8.84RR89 pKa = 11.84PPEE92 pKa = 3.72RR93 pKa = 11.84HH94 pKa = 6.26PGRR97 pKa = 11.84NN98 pKa = 3.21

Molecular weight: 11.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

16441

44

816

171.3

18.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.568 ± 0.395

0.839 ± 0.104

6.459 ± 0.177

6.782 ± 0.344

3.534 ± 0.192

8.461 ± 0.381

1.934 ± 0.156

5.091 ± 0.189

4.854 ± 0.255

7.852 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.124

3.522 ± 0.171

5.322 ± 0.204

3.406 ± 0.155

6.289 ± 0.371

5.383 ± 0.218

6.095 ± 0.285

7.129 ± 0.23

2.184 ± 0.113

2.889 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski