Phenylobacterium parvum
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2596 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z3HNY5|A0A2Z3HNY5_9CAUL DUF1289 domain-containing protein OS=Phenylobacterium parvum OX=2201350 GN=HYN04_04015 PE=4 SV=1
MM1 pKa = 6.59 GTYY4 pKa = 10.08 YY5 pKa = 10.88 YY6 pKa = 10.72 AGHH9 pKa = 7.17 TYY11 pKa = 10.41 QIVKK15 pKa = 8.82 TAMTWAAAKK24 pKa = 10.02 AWAEE28 pKa = 4.14 SQGGHH33 pKa = 6.4 LAYY36 pKa = 8.99 ITSAAEE42 pKa = 3.8 NQALVSMTQLTTGLDD57 pKa = 4.0 LAPSAADD64 pKa = 3.4 GGGARR69 pKa = 11.84 YY70 pKa = 8.77 LWLGGSDD77 pKa = 3.72 ADD79 pKa = 5.5 VEE81 pKa = 4.88 GTWRR85 pKa = 11.84 WGDD88 pKa = 3.47 GTLVTSGYY96 pKa = 9.68 SNWGAGALGVEE107 pKa = 4.24 PDD109 pKa = 3.7 NYY111 pKa = 11.15 GGVQDD116 pKa = 5.06 AMAFGLQSWPQPSGGIGTAFKK137 pKa = 10.63 WNDD140 pKa = 3.05 INPANSLYY148 pKa = 10.57 FVVEE152 pKa = 3.73 WDD154 pKa = 4.21 HH155 pKa = 7.8 IRR157 pKa = 11.84 GTTGADD163 pKa = 3.5 SISQTGNATVYY174 pKa = 10.58 GLEE177 pKa = 4.25 GNDD180 pKa = 4.38 IISLAQGSNYY190 pKa = 9.74 LRR192 pKa = 11.84 GDD194 pKa = 3.59 AGDD197 pKa = 4.13 DD198 pKa = 3.79 SVFGGSGFDD207 pKa = 4.96 DD208 pKa = 3.42 INGNMGADD216 pKa = 3.54 TLRR219 pKa = 11.84 GNDD222 pKa = 3.63 GEE224 pKa = 4.26 DD225 pKa = 2.89 WVVGGKK231 pKa = 10.32 DD232 pKa = 3.05 NDD234 pKa = 4.99 LIFGDD239 pKa = 3.73 AAFDD243 pKa = 3.41 IVYY246 pKa = 10.68 GNMGNDD252 pKa = 3.62 TVDD255 pKa = 3.22 GGAGNDD261 pKa = 3.47 WVRR264 pKa = 11.84 GGQGDD269 pKa = 4.02 DD270 pKa = 3.76 SVVSGAGDD278 pKa = 3.21 DD279 pKa = 3.97 WLWGDD284 pKa = 4.54 RR285 pKa = 11.84 GNDD288 pKa = 3.95 TISGGAGADD297 pKa = 3.45 LFYY300 pKa = 11.15 SFAGAGIDD308 pKa = 3.86 RR309 pKa = 11.84 ITDD312 pKa = 3.67 FSYY315 pKa = 11.63 AEE317 pKa = 4.05 GDD319 pKa = 3.57 RR320 pKa = 11.84 LKK322 pKa = 11.36 LEE324 pKa = 4.64 GGPSYY329 pKa = 11.06 SVSQVGADD337 pKa = 3.54 VIVDD341 pKa = 3.89 MGNGDD346 pKa = 3.66 QVILVGVSLASLGSGWILL364 pKa = 3.58
Molecular weight: 37.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.643
IPC_protein 3.694
Toseland 3.452
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.694
Rodwell 3.516
Grimsley 3.363
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.139
Thurlkill 3.516
EMBOSS 3.694
Sillero 3.821
Patrickios 1.875
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A2Z3HYR7|A0A2Z3HYR7_9CAUL TetR/AcrR family transcriptional regulator OS=Phenylobacterium parvum OX=2201350 GN=HYN04_11110 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 3.78 GWRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.5 NGRR28 pKa = 11.84 KK29 pKa = 9.27 VIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.46 GRR39 pKa = 11.84 KK40 pKa = 8.99 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2596
0
2596
863946
28
4068
332.8
35.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.986 ± 0.067
0.765 ± 0.015
5.745 ± 0.039
5.69 ± 0.058
3.531 ± 0.03
9.696 ± 0.11
1.665 ± 0.026
4.061 ± 0.024
2.478 ± 0.035
10.351 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.174 ± 0.027
2.193 ± 0.041
5.915 ± 0.059
2.784 ± 0.026
8.037 ± 0.077
5.142 ± 0.061
4.992 ± 0.075
7.425 ± 0.037
1.411 ± 0.023
1.958 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here