Porcine stool-associated circular virus 7
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076VJL0|A0A076VJL0_9VIRU Cap protein OS=Porcine stool-associated circular virus 7 OX=1537166 PE=4 SV=1
MM1 pKa = 7.22 ATNFATASYY10 pKa = 10.64 QEE12 pKa = 4.43 VVDD15 pKa = 4.2 LHH17 pKa = 6.07 TEE19 pKa = 4.0 SKK21 pKa = 8.04 TVSVIGIHH29 pKa = 5.77 TPNTSTPVKK38 pKa = 9.44 MLQGFWKK45 pKa = 10.02 QFRR48 pKa = 11.84 KK49 pKa = 9.98 VKK51 pKa = 10.76 YY52 pKa = 9.31 LGCSLSLVPAARR64 pKa = 11.84 LPADD68 pKa = 4.01 PLQVSIGAGEE78 pKa = 3.98 PTIDD82 pKa = 4.39 PRR84 pKa = 11.84 DD85 pKa = 3.69 MLNPIMFHH93 pKa = 6.27 GCHH96 pKa = 6.47 GDD98 pKa = 3.58 DD99 pKa = 3.94 MGAILDD105 pKa = 3.85 TLYY108 pKa = 11.01 SGEE111 pKa = 4.22 AVGTSDD117 pKa = 4.66 VVRR120 pKa = 11.84 KK121 pKa = 10.06 QSDD124 pKa = 3.75 SAVLDD129 pKa = 3.75 VFSEE133 pKa = 4.18 NQKK136 pKa = 10.92 GNDD139 pKa = 3.7 YY140 pKa = 11.28 VDD142 pKa = 3.18 ALYY145 pKa = 10.84 YY146 pKa = 10.51 RR147 pKa = 11.84 SLTDD151 pKa = 3.4 RR152 pKa = 11.84 TWAKK156 pKa = 10.43 AHH158 pKa = 5.87 PQVGFRR164 pKa = 11.84 KK165 pKa = 9.91 SGLRR169 pKa = 11.84 PLVHH173 pKa = 6.45 QIVANRR179 pKa = 11.84 PFSQFSPDD187 pKa = 3.3 EE188 pKa = 4.07 RR189 pKa = 11.84 SGNVGPVVVDD199 pKa = 3.71 HH200 pKa = 6.3 GTSHH204 pKa = 7.5 ASGQIGININNGSPTVSAVPGLPDD228 pKa = 3.33 TNAVEE233 pKa = 4.39 TEE235 pKa = 4.15 VNFDD239 pKa = 3.07 IGNGGGVGFAFATSGLRR256 pKa = 11.84 PLGWQDD262 pKa = 3.34 TQSSVMTSSEE272 pKa = 4.42 YY273 pKa = 10.69 VSEE276 pKa = 3.97 PLIGNAEE283 pKa = 3.67 QDD285 pKa = 3.51 AQKK288 pKa = 10.67 VANVWKK294 pKa = 10.42 QVVVPNTIPKK304 pKa = 9.8 FYY306 pKa = 9.87 MGMILLPPAYY316 pKa = 8.63 KK317 pKa = 9.67 TEE319 pKa = 3.48 QWFRR323 pKa = 11.84 LQINHH328 pKa = 5.9 NFAFKK333 pKa = 10.35 GFRR336 pKa = 11.84 GISMIDD342 pKa = 3.86 DD343 pKa = 4.09 DD344 pKa = 5.78 PEE346 pKa = 4.46 VINNAPAYY354 pKa = 9.84 TNFQQ358 pKa = 3.25
Molecular weight: 38.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.424
IPC2_protein 5.347
IPC_protein 5.347
Toseland 5.664
ProMoST 5.626
Dawson 5.525
Bjellqvist 5.575
Wikipedia 5.499
Rodwell 5.474
Grimsley 5.804
Solomon 5.525
Lehninger 5.499
Nozaki 5.741
DTASelect 5.944
Thurlkill 5.83
EMBOSS 5.804
Sillero 5.83
Patrickios 4.101
IPC_peptide 5.537
IPC2_peptide 5.83
IPC2.peptide.svr19 5.823
Protein with the highest isoelectric point:
>tr|A0A076VJL0|A0A076VJL0_9VIRU Cap protein OS=Porcine stool-associated circular virus 7 OX=1537166 PE=4 SV=1
MM1 pKa = 7.32 TKK3 pKa = 8.3 TWMLTVPRR11 pKa = 11.84 NNTAKK16 pKa = 9.95 EE17 pKa = 3.75 WVAISKK23 pKa = 8.8 WLRR26 pKa = 11.84 ANDD29 pKa = 3.33 VHH31 pKa = 8.35 KK32 pKa = 9.84 WICAMEE38 pKa = 4.31 TGADD42 pKa = 5.08 GYY44 pKa = 9.42 DD45 pKa = 2.85 HH46 pKa = 6.3 WQIRR50 pKa = 11.84 LQVNKK55 pKa = 8.64 TWEE58 pKa = 4.05 KK59 pKa = 10.86 LKK61 pKa = 10.98 EE62 pKa = 3.87 EE63 pKa = 4.62 WGPKK67 pKa = 9.21 AHH69 pKa = 6.92 IEE71 pKa = 4.06 EE72 pKa = 5.01 ASDD75 pKa = 2.99 VWDD78 pKa = 3.8 YY79 pKa = 11.22 EE80 pKa = 4.35 RR81 pKa = 11.84 KK82 pKa = 9.86 SGLFFSSQDD91 pKa = 3.26 TPEE94 pKa = 3.72 VRR96 pKa = 11.84 KK97 pKa = 10.18 CRR99 pKa = 11.84 FGHH102 pKa = 4.92 LTWRR106 pKa = 11.84 QKK108 pKa = 10.92 AVLRR112 pKa = 11.84 AVQSTNDD119 pKa = 3.42 RR120 pKa = 11.84 QVVVWYY126 pKa = 10.15 DD127 pKa = 3.21 PDD129 pKa = 4.09 GNKK132 pKa = 10.05 GKK134 pKa = 10.18 SWLLGHH140 pKa = 7.53 LYY142 pKa = 8.31 EE143 pKa = 5.2 TGQAWVVQAQDD154 pKa = 3.45 TVKK157 pKa = 11.21 GIIQDD162 pKa = 3.64 VASEE166 pKa = 4.4 YY167 pKa = 10.39 INHH170 pKa = 6.35 GWRR173 pKa = 11.84 PMVVIDD179 pKa = 5.68 IPRR182 pKa = 11.84 TWRR185 pKa = 11.84 WTSDD189 pKa = 3.05 LYY191 pKa = 11.47 VAIEE195 pKa = 4.27 RR196 pKa = 11.84 IKK198 pKa = 11.05 DD199 pKa = 3.64 GLIKK203 pKa = 10.46 DD204 pKa = 3.78 PRR206 pKa = 11.84 YY207 pKa = 9.95 SSKK210 pKa = 9.35 TVHH213 pKa = 5.66 IRR215 pKa = 11.84 GVKK218 pKa = 9.81 ILVTCNTMPKK228 pKa = 9.74 LDD230 pKa = 4.44 KK231 pKa = 10.84 LSEE234 pKa = 4.18 DD235 pKa = 2.8 RR236 pKa = 11.84 WIIIDD241 pKa = 3.8 GDD243 pKa = 3.56 ALEE246 pKa = 4.72 RR247 pKa = 11.84 LRR249 pKa = 11.84 SAWRR253 pKa = 11.84 IGEE256 pKa = 4.46 GEE258 pKa = 4.05 HH259 pKa = 7.23 SLTT262 pKa = 4.57
Molecular weight: 30.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.98
IPC2_protein 8.039
IPC_protein 8.024
Toseland 8.507
ProMoST 8.478
Dawson 8.858
Bjellqvist 8.843
Wikipedia 8.902
Rodwell 8.99
Grimsley 8.492
Solomon 9.048
Lehninger 9.033
Nozaki 9.019
DTASelect 8.712
Thurlkill 8.843
EMBOSS 9.048
Sillero 9.048
Patrickios 4.902
IPC_peptide 9.033
IPC2_peptide 7.571
IPC2.peptide.svr19 7.595
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
620
262
358
310.0
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.613 ± 0.545
0.806 ± 0.208
6.613 ± 0.392
4.677 ± 0.877
3.226 ± 1.277
7.581 ± 0.904
2.742 ± 0.191
5.806 ± 0.653
5.484 ± 1.319
6.452 ± 0.257
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.258 ± 0.215
4.677 ± 0.996
5.161 ± 1.293
4.516 ± 0.429
5.161 ± 1.283
6.774 ± 0.878
6.29 ± 0.122
8.871 ± 0.525
3.548 ± 1.804
2.742 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here