Enterococcus phage VFW

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5SAB1|A0A1G5SAB1_9CAUD Uncharacterized protein OS=Enterococcus phage VFW OX=1813888 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.07IEE4 pKa = 4.05KK5 pKa = 9.7EE6 pKa = 4.04KK7 pKa = 10.67LKK9 pKa = 10.91HH10 pKa = 5.63VIKK13 pKa = 10.09EE14 pKa = 3.91LEE16 pKa = 4.16YY17 pKa = 9.91EE18 pKa = 4.3VEE20 pKa = 4.43FNSPIPGIINNEE32 pKa = 4.11NIVVDD37 pKa = 3.93WEE39 pKa = 5.13DD40 pKa = 3.21IMEE43 pKa = 5.22DD44 pKa = 4.33DD45 pKa = 5.67EE46 pKa = 6.15EE47 pKa = 4.76DD48 pKa = 3.35LCEE51 pKa = 4.09ICGCEE56 pKa = 5.76HH57 pKa = 6.63IDD59 pKa = 3.22SWEE62 pKa = 4.29AGACWSSHH70 pKa = 5.76NDD72 pKa = 3.28PDD74 pKa = 4.57YY75 pKa = 11.63DD76 pKa = 4.05PFDD79 pKa = 3.3II80 pKa = 5.77

Molecular weight: 9.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5S9Y5|A0A1G5S9Y5_9CAUD Uncharacterized protein OS=Enterococcus phage VFW OX=1813888 PE=4 SV=1
MM1 pKa = 7.61IKK3 pKa = 9.31TGKK6 pKa = 9.39IEE8 pKa = 3.93KK9 pKa = 9.82EE10 pKa = 3.75KK11 pKa = 11.31LNFSLHH17 pKa = 6.45KK18 pKa = 9.81NDD20 pKa = 3.16KK21 pKa = 10.84GEE23 pKa = 4.44IIRR26 pKa = 11.84VPLKK30 pKa = 10.08NKK32 pKa = 9.98KK33 pKa = 9.22VVSRR37 pKa = 11.84QEE39 pKa = 3.58RR40 pKa = 11.84VKK42 pKa = 11.1NNKK45 pKa = 8.69IKK47 pKa = 10.7KK48 pKa = 9.04ALKK51 pKa = 9.62KK52 pKa = 9.98RR53 pKa = 11.84KK54 pKa = 9.21KK55 pKa = 10.19

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

25960

38

1288

201.2

23.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.844 ± 0.335

0.682 ± 0.074

6.514 ± 0.164

8.54 ± 0.24

4.372 ± 0.146

5.828 ± 0.246

1.495 ± 0.109

7.754 ± 0.189

9.272 ± 0.222

8.394 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.047 ± 0.098

6.576 ± 0.165

2.527 ± 0.127

3.498 ± 0.16

3.567 ± 0.147

5.116 ± 0.179

5.293 ± 0.249

6.194 ± 0.16

1.213 ± 0.146

4.276 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski