Cricetibacter osteomyelitidis
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2525 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R2SX63|A0A4R2SX63_9PAST Na(+)-translocating NADH-quinone reductase subunit B OS=Cricetibacter osteomyelitidis OX=1521931 GN=nqrB PE=3 SV=1
MM1 pKa = 7.58 AIVGVFYY8 pKa = 10.81 GSDD11 pKa = 3.07 TGNTEE16 pKa = 4.47 NIAKK20 pKa = 9.24 MIQKK24 pKa = 9.58 QLGNEE29 pKa = 3.93 LVDD32 pKa = 3.33 IRR34 pKa = 11.84 DD35 pKa = 3.34 IAKK38 pKa = 10.28 SSKK41 pKa = 10.46 EE42 pKa = 3.92 DD43 pKa = 3.08 IEE45 pKa = 4.87 AYY47 pKa = 10.47 NFLLIGIPTWYY58 pKa = 10.2 YY59 pKa = 11.6 GEE61 pKa = 4.69 AQCDD65 pKa = 3.26 WDD67 pKa = 5.24 DD68 pKa = 4.33 FFPTLEE74 pKa = 5.54 DD75 pKa = 3.08 IDD77 pKa = 4.52 FSEE80 pKa = 4.57 KK81 pKa = 10.26 LVAIFGCGDD90 pKa = 3.22 QEE92 pKa = 4.63 DD93 pKa = 4.32 YY94 pKa = 11.51 SEE96 pKa = 4.47 YY97 pKa = 10.75 FCDD100 pKa = 4.41 AMGTVRR106 pKa = 11.84 DD107 pKa = 3.94 IIEE110 pKa = 4.2 PRR112 pKa = 11.84 GATIVGHH119 pKa = 6.6 WPTEE123 pKa = 3.83 GYY125 pKa = 8.73 TFDD128 pKa = 3.61 EE129 pKa = 4.76 SKK131 pKa = 11.43 ALVDD135 pKa = 4.35 DD136 pKa = 3.88 NTFVGLCIDD145 pKa = 4.19 EE146 pKa = 4.82 DD147 pKa = 3.92 RR148 pKa = 11.84 QPEE151 pKa = 4.13 LTAEE155 pKa = 4.36 RR156 pKa = 11.84 VKK158 pKa = 10.6 QWVNQVYY165 pKa = 11.02 DD166 pKa = 4.15 EE167 pKa = 4.53 MCLSEE172 pKa = 4.39 LAA174 pKa = 4.16
Molecular weight: 19.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.656
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.567
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.151
Thurlkill 3.694
EMBOSS 3.77
Sillero 3.973
Patrickios 1.888
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A4R2T8G9|A0A4R2T8G9_9PAST AraC family transcriptional regulator OS=Cricetibacter osteomyelitidis OX=1521931 GN=EDC44_1368 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2525
0
2525
819869
27
2869
324.7
36.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.41 ± 0.051
0.999 ± 0.018
5.135 ± 0.05
6.025 ± 0.049
4.41 ± 0.044
6.781 ± 0.051
2.03 ± 0.024
7.165 ± 0.044
6.081 ± 0.053
10.332 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.423 ± 0.025
5.021 ± 0.052
3.723 ± 0.026
4.73 ± 0.041
4.269 ± 0.04
5.828 ± 0.042
5.444 ± 0.072
6.643 ± 0.046
1.189 ± 0.02
3.36 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here