Streptococcus satellite phage Javan736
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZVH1|A0A4D5ZVH1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan736 OX=2558830 GN=JavanS736_0002 PE=4 SV=1
MM1 pKa = 7.44 NNKK4 pKa = 8.74 QVYY7 pKa = 8.26 QAQTTDD13 pKa = 2.91 HH14 pKa = 6.88 GIARR18 pKa = 11.84 ALGEE22 pKa = 4.87 EE23 pKa = 3.69 IRR25 pKa = 11.84 MITDD29 pKa = 3.35 VEE31 pKa = 4.09 EE32 pKa = 4.08 TTYY35 pKa = 11.03 NGHH38 pKa = 6.1 GEE40 pKa = 4.18 IIVEE44 pKa = 4.21 YY45 pKa = 9.21 TSDD48 pKa = 3.37 VALTKK53 pKa = 10.9 LFDD56 pKa = 4.94 RR57 pKa = 11.84 IKK59 pKa = 10.69 ALYY62 pKa = 9.16 EE63 pKa = 3.92 KK64 pKa = 10.84 GEE66 pKa = 4.25 SYY68 pKa = 7.74 EE69 pKa = 4.29 TKK71 pKa = 10.16 PFLHH75 pKa = 6.55 FKK77 pKa = 10.6 VLEE80 pKa = 4.35 DD81 pKa = 5.71 DD82 pKa = 5.05 IEE84 pKa = 4.33 PSPNGVFTAKK94 pKa = 9.9 EE95 pKa = 3.79 VAEE98 pKa = 4.05 FLGII102 pKa = 3.93
Molecular weight: 11.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.681
IPC2_protein 4.711
IPC_protein 4.584
Toseland 4.457
ProMoST 4.673
Dawson 4.52
Bjellqvist 4.673
Wikipedia 4.368
Rodwell 4.431
Grimsley 4.368
Solomon 4.52
Lehninger 4.469
Nozaki 4.635
DTASelect 4.736
Thurlkill 4.444
EMBOSS 4.393
Sillero 4.698
Patrickios 3.834
IPC_peptide 4.52
IPC2_peptide 4.698
IPC2.peptide.svr19 4.619
Protein with the highest isoelectric point:
>tr|A0A4D5ZVH5|A0A4D5ZVH5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan736 OX=2558830 GN=JavanS736_0015 PE=4 SV=1
MM1 pKa = 7.25 KK2 pKa = 10.36 RR3 pKa = 11.84 IATIFLLGLPKK14 pKa = 9.96 VPKK17 pKa = 8.88 MRR19 pKa = 11.84 PIAYY23 pKa = 8.55 PRR25 pKa = 11.84 ATISNNPEE33 pKa = 3.14 ARR35 pKa = 11.84 GIYY38 pKa = 10.11 LEE40 pKa = 4.06 NKK42 pKa = 9.02 KK43 pKa = 10.62
Molecular weight: 4.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.286
IPC2_protein 10.058
IPC_protein 10.716
Toseland 10.935
ProMoST 10.657
Dawson 11.023
Bjellqvist 10.687
Wikipedia 11.199
Rodwell 11.418
Grimsley 11.052
Solomon 11.125
Lehninger 11.096
Nozaki 10.906
DTASelect 10.687
Thurlkill 10.921
EMBOSS 11.33
Sillero 10.935
Patrickios 11.199
IPC_peptide 11.125
IPC2_peptide 9.399
IPC2.peptide.svr19 8.309
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
3423
43
492
148.8
17.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.668 ± 0.244
0.789 ± 0.18
5.521 ± 0.467
8.969 ± 0.709
4.178 ± 0.312
5.229 ± 0.376
1.548 ± 0.246
6.515 ± 0.451
9.378 ± 0.673
10.079 ± 0.519
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.688 ± 0.27
5.229 ± 0.476
3.184 ± 0.448
3.593 ± 0.279
5.726 ± 0.501
5.112 ± 0.31
6.135 ± 0.429
5.2 ± 0.388
0.789 ± 0.182
4.47 ± 0.352
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here