Mycobacterium phage Bluefalcon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WA85|A0A5Q2WA85_9CAUD Uncharacterized protein OS=Mycobacterium phage Bluefalcon OX=2664224 GN=71 PE=4 SV=1
MM1 pKa = 7.24ARR3 pKa = 11.84IYY5 pKa = 10.83VADD8 pKa = 4.16LAAYY12 pKa = 9.94NKK14 pKa = 10.42GDD16 pKa = 5.09LVGKK20 pKa = 9.35WIDD23 pKa = 3.54LDD25 pKa = 3.54QFSDD29 pKa = 3.87VEE31 pKa = 4.06EE32 pKa = 4.79LEE34 pKa = 4.45WEE36 pKa = 4.31IKK38 pKa = 8.54THH40 pKa = 5.46VLLDD44 pKa = 3.71GHH46 pKa = 6.12EE47 pKa = 3.98EE48 pKa = 4.0WAIHH52 pKa = 5.12DD53 pKa = 3.93HH54 pKa = 6.47EE55 pKa = 5.16GFGGLRR61 pKa = 11.84ISEE64 pKa = 4.48HH65 pKa = 6.58EE66 pKa = 4.61DD67 pKa = 3.31LDD69 pKa = 4.12VMLEE73 pKa = 3.88LSEE76 pKa = 5.07AVEE79 pKa = 3.97KK80 pKa = 11.14HH81 pKa = 5.72GDD83 pKa = 3.7PFVAWIGDD91 pKa = 3.54IVGDD95 pKa = 3.4IGYY98 pKa = 9.64FGSVSEE104 pKa = 5.23AVDD107 pKa = 3.33QFEE110 pKa = 4.73DD111 pKa = 4.28VYY113 pKa = 10.46IGQMTIEE120 pKa = 4.74DD121 pKa = 3.87YY122 pKa = 10.6AYY124 pKa = 9.76EE125 pKa = 4.08YY126 pKa = 11.32AEE128 pKa = 4.34EE129 pKa = 4.16VLGLTGTALDD139 pKa = 4.04YY140 pKa = 11.27FDD142 pKa = 4.14TEE144 pKa = 3.89KK145 pKa = 10.57FARR148 pKa = 11.84DD149 pKa = 3.51LEE151 pKa = 4.42ASGDD155 pKa = 3.9VSEE158 pKa = 4.21VWYY161 pKa = 11.18NGDD164 pKa = 3.55YY165 pKa = 10.99YY166 pKa = 11.2VFRR169 pKa = 11.84AWW171 pKa = 3.42

Molecular weight: 19.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WC64|A0A5Q2WC64_9CAUD Lysin B OS=Mycobacterium phage Bluefalcon OX=2664224 GN=7 PE=4 SV=1
MM1 pKa = 6.95TAAHH5 pKa = 6.68SLPSDD10 pKa = 2.9WTTDD14 pKa = 2.59KK15 pKa = 10.8RR16 pKa = 11.84RR17 pKa = 11.84QVGMNKK23 pKa = 7.34QQRR26 pKa = 11.84VYY28 pKa = 11.11LDD30 pKa = 3.04GKK32 pKa = 10.56VYY34 pKa = 10.09VARR37 pKa = 11.84GNHH40 pKa = 4.97IVRR43 pKa = 11.84IRR45 pKa = 3.18

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15816

32

1113

192.9

21.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.358 ± 0.337

0.866 ± 0.12

7.069 ± 0.26

7.461 ± 0.333

3.364 ± 0.199

8.447 ± 0.468

1.878 ± 0.144

5.014 ± 0.152

4.47 ± 0.296

8.087 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.119

3.364 ± 0.163

5.058 ± 0.27

3.383 ± 0.208

6.045 ± 0.302

5.273 ± 0.202

6.051 ± 0.333

7.391 ± 0.293

2.055 ± 0.153

3.085 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski