Alcanivorax pacificus W11-5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Alcanivoracaceae; Alcanivorax; Alcanivorax pacificus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4XMT0|A0A0B4XMT0_9GAMM LysR family transcriptional regulator OS=Alcanivorax pacificus W11-5 OX=391936 GN=S7S_07085 PE=3 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84LKK4 pKa = 10.13WLFGLCLSLLLATHH18 pKa = 6.85AQADD22 pKa = 4.18NYY24 pKa = 10.69FLTITNSTGFDD35 pKa = 2.71ILYY38 pKa = 9.93AYY40 pKa = 9.74VSPEE44 pKa = 4.06HH45 pKa = 6.89SDD47 pKa = 3.01EE48 pKa = 4.12WEE50 pKa = 3.96EE51 pKa = 4.59DD52 pKa = 3.62VLGTDD57 pKa = 3.24ILEE60 pKa = 4.98DD61 pKa = 3.77GASVRR66 pKa = 11.84ITLQGYY72 pKa = 8.49DD73 pKa = 3.32SPLFDD78 pKa = 3.97VRR80 pKa = 11.84LVDD83 pKa = 5.1EE84 pKa = 6.07DD85 pKa = 4.91DD86 pKa = 3.56DD87 pKa = 4.97TYY89 pKa = 11.59TFWGVNVAKK98 pKa = 10.3QDD100 pKa = 3.84LEE102 pKa = 4.38VTLDD106 pKa = 3.58HH107 pKa = 7.43LDD109 pKa = 3.29

Molecular weight: 12.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4XSH5|A0A0B4XSH5_9GAMM Flagellar hook protein FlgE OS=Alcanivorax pacificus W11-5 OX=391936 GN=S7S_14805 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQIKK11 pKa = 10.05RR12 pKa = 11.84KK13 pKa = 7.74RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84AVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 8.12QLTVV44 pKa = 2.97

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3686

0

3686

1228667

33

13341

333.3

36.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.215 ± 0.049

0.958 ± 0.015

5.956 ± 0.042

5.723 ± 0.041

3.483 ± 0.031

8.169 ± 0.086

2.353 ± 0.021

4.445 ± 0.031

2.541 ± 0.037

11.468 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.023

2.783 ± 0.025

5.08 ± 0.034

4.244 ± 0.027

7.341 ± 0.05

5.321 ± 0.038

5.193 ± 0.037

7.321 ± 0.041

1.482 ± 0.017

2.489 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski