Eidolon helvum parvovirus 1 isolate Bat/Ghana/-/2009
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H2ESE6|H2ESE6_BTPV1 MARF OS=Eidolon helvum parvovirus 1 (isolate Bat/Ghana/-/2009) OX=1560037 GN=MARF PE=4 SV=1
MM1 pKa = 7.71 ASGGVAFFMPTGEE14 pKa = 4.14 GGKK17 pKa = 10.18 AISKK21 pKa = 10.25 LYY23 pKa = 10.79 DD24 pKa = 3.41 GAVTGWSQSHH34 pKa = 6.4 LRR36 pKa = 11.84 HH37 pKa = 4.44 GHH39 pKa = 4.75 YY40 pKa = 10.14 RR41 pKa = 11.84 ATALSGLVYY50 pKa = 10.46 QKK52 pKa = 11.23 AFEE55 pKa = 4.33 NLDD58 pKa = 3.42 AFAKK62 pKa = 10.14 HH63 pKa = 6.19 LPPSLRR69 pKa = 11.84 LVQTLYY75 pKa = 11.59 NNISKK80 pKa = 9.65 QRR82 pKa = 11.84 NKK84 pKa = 9.17 TANTEE89 pKa = 3.88 WWWRR93 pKa = 11.84 VYY95 pKa = 10.0 TDD97 pKa = 3.54 CTRR100 pKa = 11.84 LLLTVAQPQAADD112 pKa = 3.38 ALRR115 pKa = 11.84 RR116 pKa = 11.84 AWNGTSPLRR125 pKa = 11.84 PPAQQVLQNVYY136 pKa = 7.93 MLAYY140 pKa = 9.56 NSHH143 pKa = 6.34 IPSEE147 pKa = 4.13 EE148 pKa = 3.95 DD149 pKa = 3.11 LNTFFDD155 pKa = 4.02 EE156 pKa = 4.49 SVLGSVSGDD165 pKa = 3.3 PEE167 pKa = 3.52 NFAFVKK173 pKa = 9.94 QAVVSAFHH181 pKa = 6.79 PADD184 pKa = 3.67 GGAATEE190 pKa = 4.1 QRR192 pKa = 11.84 SDD194 pKa = 3.66 VPEE197 pKa = 4.49 SPSPPSEE204 pKa = 4.1 SDD206 pKa = 2.75 HH207 pKa = 6.05 SQPSGGGLLVPGYY220 pKa = 10.84 NYY222 pKa = 10.28 VGPGNPLDD230 pKa = 4.14 NGPPKK235 pKa = 10.92 GPVDD239 pKa = 3.48 EE240 pKa = 4.81 AARR243 pKa = 11.84 NHH245 pKa = 5.92 DD246 pKa = 3.31 RR247 pKa = 11.84 RR248 pKa = 11.84 YY249 pKa = 11.27 DD250 pKa = 3.49 EE251 pKa = 4.53 MLSHH255 pKa = 7.15 GDD257 pKa = 3.43 VPYY260 pKa = 10.98 LDD262 pKa = 4.03 SQGVDD267 pKa = 2.97 QLMTKK272 pKa = 10.17 EE273 pKa = 4.24 IEE275 pKa = 4.22 DD276 pKa = 3.94 AEE278 pKa = 4.48 KK279 pKa = 10.75 KK280 pKa = 10.46 EE281 pKa = 3.93 PLGPIDD287 pKa = 4.78 ALVANAARR295 pKa = 11.84 ALWKK299 pKa = 10.64 GKK301 pKa = 7.71 EE302 pKa = 4.11 TLASVIGDD310 pKa = 3.27 QGHH313 pKa = 5.49 QVLPPNPPSVDD324 pKa = 3.27 QGSHH328 pKa = 6.27 LGSKK332 pKa = 10.35 RR333 pKa = 11.84 PLEE336 pKa = 4.11 EE337 pKa = 4.16 SEE339 pKa = 4.56 EE340 pKa = 4.3 NSDD343 pKa = 3.94 SLAKK347 pKa = 10.32 KK348 pKa = 9.46 FRR350 pKa = 11.84 PGSSPKK356 pKa = 10.12 SDD358 pKa = 4.1 LEE360 pKa = 4.23 PSGGTAPTAPPKK372 pKa = 10.19 GATMSTEE379 pKa = 4.11 AEE381 pKa = 4.29 GSGGGIKK388 pKa = 10.19 VKK390 pKa = 10.67 ALWTGGTEE398 pKa = 4.18 FSDD401 pKa = 3.78 TSIHH405 pKa = 5.85 TSHH408 pKa = 6.51 TRR410 pKa = 11.84 VALLADD416 pKa = 4.48 RR417 pKa = 11.84 GTYY420 pKa = 8.58 MPIYY424 pKa = 10.12 RR425 pKa = 11.84 PGEE428 pKa = 4.2 TTSVIQPLLGMVTPYY443 pKa = 10.98 SYY445 pKa = 10.96 IDD447 pKa = 3.74 VNSLSAHH454 pKa = 5.52 LTPRR458 pKa = 11.84 DD459 pKa = 3.66 FQQLIDD465 pKa = 4.08 EE466 pKa = 4.57 YY467 pKa = 11.71 GEE469 pKa = 3.95 IRR471 pKa = 11.84 PKK473 pKa = 10.79 SLTIGISGIVVKK485 pKa = 10.53 DD486 pKa = 3.25 VSVNTTGTAVTDD498 pKa = 3.77 SGSGGITVFADD509 pKa = 2.94 EE510 pKa = 5.56 GYY512 pKa = 10.61 DD513 pKa = 3.5 YY514 pKa = 10.84 PYY516 pKa = 11.53 VLGHH520 pKa = 6.2 NQDD523 pKa = 3.85 TLPGHH528 pKa = 7.2 LPGEE532 pKa = 4.69 HH533 pKa = 6.1 YY534 pKa = 11.06 VLPQYY539 pKa = 10.85 AYY541 pKa = 7.83 CTRR544 pKa = 11.84 GRR546 pKa = 11.84 EE547 pKa = 3.9 VVNANKK553 pKa = 9.73 IDD555 pKa = 4.13 IIQDD559 pKa = 2.9 HH560 pKa = 5.95 RR561 pKa = 11.84 TDD563 pKa = 4.11 FFLLEE568 pKa = 4.02 HH569 pKa = 7.08 HH570 pKa = 7.43 DD571 pKa = 3.95 AXCLNSGDD579 pKa = 4.23 TWSHH583 pKa = 6.09 TYY585 pKa = 10.67 SFPDD589 pKa = 3.35 LPFRR593 pKa = 11.84 RR594 pKa = 11.84 LTYY597 pKa = 10.04 PSQHH601 pKa = 6.79 LYY603 pKa = 9.59 AQHH606 pKa = 6.06 NPQMKK611 pKa = 9.74 SRR613 pKa = 11.84 LGVFRR618 pKa = 11.84 SVDD621 pKa = 3.66 QNGDD625 pKa = 3.78 PAWQRR630 pKa = 11.84 LRR632 pKa = 11.84 GSDD635 pKa = 4.25 IGQLPCNYY643 pKa = 10.11 LPGPQANLPTTSDD656 pKa = 3.75 FQKK659 pKa = 10.72 GDD661 pKa = 3.24 SMLPAAIGDD670 pKa = 4.72 PITGDD675 pKa = 3.25 RR676 pKa = 11.84 YY677 pKa = 8.09 TVTPLVHH684 pKa = 6.16 QPWSMVHH691 pKa = 7.23 DD692 pKa = 4.96 EE693 pKa = 4.5 YY694 pKa = 11.76 NNTGQRR700 pKa = 11.84 TQSVVSTVGSVAYY713 pKa = 8.96 MPRR716 pKa = 11.84 KK717 pKa = 9.77 HH718 pKa = 6.21 EE719 pKa = 4.07 EE720 pKa = 4.34 SYY722 pKa = 11.4 GSPNDD727 pKa = 3.6 ALTEE731 pKa = 3.79 ITTPMRR737 pKa = 11.84 VVQEE741 pKa = 3.99 QADD744 pKa = 4.23 LVSSRR749 pKa = 11.84 LGHH752 pKa = 5.85 TFMIPSFSRR761 pKa = 11.84 KK762 pKa = 9.45 AGSASTTTYY771 pKa = 10.51 NEE773 pKa = 3.73 PMFPLLPGAVWNPNPLTYY791 pKa = 10.47 DD792 pKa = 3.39 CQIWCKK798 pKa = 10.76 VPDD801 pKa = 4.11 TEE803 pKa = 5.14 CKK805 pKa = 10.51 FMTQYY810 pKa = 11.1 PLLGGWGMDD819 pKa = 3.83 TPPPMIFLRR828 pKa = 11.84 IRR830 pKa = 11.84 RR831 pKa = 11.84 QPGPPGPGAHH841 pKa = 5.63 TVPGSTLNQYY851 pKa = 10.73 VIFHH855 pKa = 6.48 LHH857 pKa = 5.43 YY858 pKa = 11.1 SMEE861 pKa = 4.22 FEE863 pKa = 3.9 VRR865 pKa = 11.84 RR866 pKa = 11.84 RR867 pKa = 11.84 KK868 pKa = 9.8 RR869 pKa = 11.84 SRR871 pKa = 11.84 RR872 pKa = 11.84 HH873 pKa = 4.96 NPEE876 pKa = 3.3 MPAPFPQTWSGKK888 pKa = 8.12 MPYY891 pKa = 9.11 TLSTDD896 pKa = 3.29 GKK898 pKa = 10.55 SGPEE902 pKa = 3.72 YY903 pKa = 10.54 NVPRR907 pKa = 11.84 DD908 pKa = 3.17 QWVARR913 pKa = 11.84 NYY915 pKa = 10.72 SQLLL919 pKa = 3.6
Molecular weight: 100.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.681
IPC2_protein 5.588
IPC_protein 5.664
Toseland 6.16
ProMoST 6.033
Dawson 5.944
Bjellqvist 5.944
Wikipedia 5.957
Rodwell 5.919
Grimsley 6.364
Solomon 5.944
Lehninger 5.931
Nozaki 6.211
DTASelect 6.402
Thurlkill 6.415
EMBOSS 6.376
Sillero 6.313
Patrickios 3.948
IPC_peptide 5.957
IPC2_peptide 6.313
IPC2.peptide.svr19 6.214
Protein with the highest isoelectric point:
>tr|H2ESE7|H2ESE7_BTPV1 Putative capsid protein VP2 OS=Eidolon helvum parvovirus 1 (isolate Bat/Ghana/-/2009) OX=1560037 PE=3 SV=1
MM1 pKa = 7.86 EE2 pKa = 5.18 EE3 pKa = 4.31 PLLNSALTSLKK14 pKa = 10.54 VLAPLVNQTIHH25 pKa = 6.54 SLAEE29 pKa = 4.15 VVSLSPATIMWVLVILWIMAPLRR52 pKa = 11.84 AQWMRR57 pKa = 11.84 QLAIMTEE64 pKa = 4.07 GMTKK68 pKa = 10.31 CFPTGTSPTT77 pKa = 3.75
Molecular weight: 8.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.386
IPC2_protein 6.148
IPC_protein 6.634
Toseland 6.605
ProMoST 6.561
Dawson 6.985
Bjellqvist 6.532
Wikipedia 6.956
Rodwell 6.927
Grimsley 6.649
Solomon 7.161
Lehninger 7.161
Nozaki 6.912
DTASelect 7.176
Thurlkill 7.205
EMBOSS 7.366
Sillero 7.278
Patrickios 4.8
IPC_peptide 7.161
IPC2_peptide 7.0
IPC2.peptide.svr19 7.027
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2232
77
919
558.0
62.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.944 ± 1.141
1.344 ± 0.407
5.421 ± 0.469
5.242 ± 0.813
3.226 ± 0.3
7.93 ± 0.603
2.912 ± 0.475
3.584 ± 0.403
3.405 ± 0.37
8.244 ± 0.6
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.583
3.539 ± 0.303
8.065 ± 0.9
4.077 ± 0.564
5.914 ± 0.68
7.706 ± 0.337
6.765 ± 1.054
6.676 ± 0.375
2.509 ± 0.729
3.987 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here