Microbulbifer sp. A4B17

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Microbulbiferaceae; Microbulbifer; unclassified Microbulbifer

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1JNE0|A0A2S1JNE0_9GAMM Long-chain fatty acid--CoA ligase OS=Microbulbifer sp. A4B17 OX=359370 GN=BTJ40_03330 PE=4 SV=1
MM1 pKa = 7.27NFSLSSKK8 pKa = 10.0VRR10 pKa = 11.84FPLKK14 pKa = 10.3PIAAVVLFFCASNEE28 pKa = 3.89AFSAPGEE35 pKa = 4.07LSDD38 pKa = 4.02VPLYY42 pKa = 11.04SRR44 pKa = 11.84GSAEE48 pKa = 4.0PNLMFVLDD56 pKa = 4.3SSGSMNEE63 pKa = 3.69MVLPVDD69 pKa = 5.35GYY71 pKa = 10.18DD72 pKa = 3.34TSDD75 pKa = 3.6PAGSSCSSPISMTYY89 pKa = 9.68TSNSCRR95 pKa = 11.84GGGSCTRR102 pKa = 11.84YY103 pKa = 10.11YY104 pKa = 11.32DD105 pKa = 3.74DD106 pKa = 5.5VEE108 pKa = 4.59YY109 pKa = 10.85QVDD112 pKa = 3.73TSDD115 pKa = 3.38GSVDD119 pKa = 2.99IKK121 pKa = 11.21LVDD124 pKa = 3.62RR125 pKa = 11.84NRR127 pKa = 11.84AEE129 pKa = 4.62SEE131 pKa = 3.71WFTWNSSSNCFDD143 pKa = 3.39EE144 pKa = 5.46DD145 pKa = 3.56EE146 pKa = 5.18NYY148 pKa = 10.75SAWLYY153 pKa = 10.43AASSSGGGSYY163 pKa = 8.82YY164 pKa = 8.57TTGSSSGYY172 pKa = 9.27YY173 pKa = 9.26RR174 pKa = 11.84EE175 pKa = 4.25TVTGHH180 pKa = 5.99FLNWFFSKK188 pKa = 10.48EE189 pKa = 4.02NSSGGYY195 pKa = 9.19EE196 pKa = 3.79ADD198 pKa = 3.74EE199 pKa = 5.5FKK201 pKa = 11.27DD202 pKa = 4.11DD203 pKa = 4.63DD204 pKa = 4.83DD205 pKa = 3.84NAARR209 pKa = 11.84SKK211 pKa = 11.03VDD213 pKa = 2.98IRR215 pKa = 11.84RR216 pKa = 11.84TDD218 pKa = 2.91IMIGAAQEE226 pKa = 4.19LVNGLDD232 pKa = 3.7DD233 pKa = 3.88VRR235 pKa = 11.84IGLMQFNSTSGARR248 pKa = 11.84LLFGLEE254 pKa = 4.09SLTDD258 pKa = 3.7SNRR261 pKa = 11.84TDD263 pKa = 2.99ILDD266 pKa = 4.03IIEE269 pKa = 5.82DD270 pKa = 3.6IDD272 pKa = 4.32ANGYY276 pKa = 7.28TPLAEE281 pKa = 4.15SFAGVGRR288 pKa = 11.84YY289 pKa = 8.56FISGYY294 pKa = 10.08EE295 pKa = 4.27GNNLTYY301 pKa = 9.92IDD303 pKa = 3.73STGAEE308 pKa = 4.2TQDD311 pKa = 3.39PGEE314 pKa = 5.03DD315 pKa = 2.9IFVGATSGNSTTTILDD331 pKa = 3.5WNNISAPDD339 pKa = 3.59NTIDD343 pKa = 4.04GGAIQYY349 pKa = 8.42YY350 pKa = 7.19CQKK353 pKa = 10.63SFMVALTDD361 pKa = 4.8GEE363 pKa = 4.34PTEE366 pKa = 5.33DD367 pKa = 4.1NSISDD372 pKa = 4.45DD373 pKa = 3.63LTGYY377 pKa = 10.91DD378 pKa = 4.25YY379 pKa = 11.68GCSSNSGGCTSDD391 pKa = 4.01EE392 pKa = 3.91MDD394 pKa = 4.22DD395 pKa = 3.99VVKK398 pKa = 10.73ALYY401 pKa = 10.58DD402 pKa = 3.28IDD404 pKa = 5.29LRR406 pKa = 11.84PDD408 pKa = 3.48LKK410 pKa = 10.56KK411 pKa = 9.18TDD413 pKa = 3.5GEE415 pKa = 4.28EE416 pKa = 4.01VVNNITSYY424 pKa = 10.81IIGFAEE430 pKa = 5.5DD431 pKa = 4.06GLSDD435 pKa = 3.7TDD437 pKa = 3.62LMKK440 pKa = 10.94NAGEE444 pKa = 4.02LGGGGVYY451 pKa = 8.77TASNATEE458 pKa = 3.94LQLTFNTIINNVQSIVGSSSSVSFNTSSLEE488 pKa = 3.86ANSAIFSASFDD499 pKa = 3.49SSAWSGDD506 pKa = 3.56LEE508 pKa = 4.57VWSLDD513 pKa = 3.41DD514 pKa = 4.38EE515 pKa = 5.09GNISSTSSWQASSEE529 pKa = 4.21LEE531 pKa = 4.5SISADD536 pKa = 3.14NRR538 pKa = 11.84VMLSYY543 pKa = 10.72RR544 pKa = 11.84GGAGVAFTASGLGTSGTDD562 pKa = 3.49HH563 pKa = 7.66ADD565 pKa = 3.71DD566 pKa = 5.21LNLDD570 pKa = 3.56IQSNAGVDD578 pKa = 3.61DD579 pKa = 3.97GRR581 pKa = 11.84ASDD584 pKa = 4.33RR585 pKa = 11.84IDD587 pKa = 3.63YY588 pKa = 10.91LRR590 pKa = 11.84GDD592 pKa = 3.53QTDD595 pKa = 3.56EE596 pKa = 3.86GTEE599 pKa = 3.85AGDD602 pKa = 3.38FRR604 pKa = 11.84VRR606 pKa = 11.84EE607 pKa = 4.1SRR609 pKa = 11.84LGDD612 pKa = 3.33IVSSTPVYY620 pKa = 10.37VGEE623 pKa = 4.85PSSSWSEE630 pKa = 4.12AEE632 pKa = 3.85FSEE635 pKa = 4.54ASSYY639 pKa = 11.83DD640 pKa = 3.35SFVSSNSSRR649 pKa = 11.84TPVVYY654 pKa = 10.55VGANDD659 pKa = 3.99GFLHH663 pKa = 6.54GFNANVSGADD673 pKa = 3.26AGKK676 pKa = 10.37EE677 pKa = 3.8LIAYY681 pKa = 9.44SPAALLSTTADD692 pKa = 3.47EE693 pKa = 4.81GLHH696 pKa = 6.2ALSSQYY702 pKa = 8.69YY703 pKa = 4.47THH705 pKa = 7.48KK706 pKa = 10.47YY707 pKa = 9.26YY708 pKa = 11.18VDD710 pKa = 3.73GTPTASDD717 pKa = 3.81AYY719 pKa = 9.94IDD721 pKa = 4.45GSWEE725 pKa = 4.05TVLVGGLGGGGKK737 pKa = 10.23GYY739 pKa = 10.65YY740 pKa = 9.98ALNITNPSDD749 pKa = 3.45FTEE752 pKa = 4.62ANAADD757 pKa = 3.12IVMWEE762 pKa = 4.51FTDD765 pKa = 4.23SDD767 pKa = 4.33NNNLGYY773 pKa = 9.25TFSRR777 pKa = 11.84PQIGRR782 pKa = 11.84MSNGEE787 pKa = 3.42WAAIFGNGYY796 pKa = 10.21NSSTGDD802 pKa = 3.02AGLFIVYY809 pKa = 10.22LDD811 pKa = 4.74GEE813 pKa = 4.46DD814 pKa = 3.57SSGNSHH820 pKa = 7.03IYY822 pKa = 10.32ISTEE826 pKa = 3.78TADD829 pKa = 3.73TDD831 pKa = 4.26DD832 pKa = 4.65KK833 pKa = 11.94NGMSTPAIVDD843 pKa = 3.37SDD845 pKa = 4.23LDD847 pKa = 3.78GTIDD851 pKa = 4.46RR852 pKa = 11.84IYY854 pKa = 11.23AGDD857 pKa = 3.56LKK859 pKa = 11.24GNMWAFDD866 pKa = 3.64VSKK869 pKa = 9.81TGSWDD874 pKa = 3.49VASSTTSPSPLFSVSGEE891 pKa = 4.39AITGAPLVARR901 pKa = 11.84NTDD904 pKa = 3.29NSSGASPNLLVAFGTGQYY922 pKa = 10.8LVDD925 pKa = 4.72SDD927 pKa = 4.65TSDD930 pKa = 3.43TNPGGFYY937 pKa = 10.54VVSDD941 pKa = 3.5NDD943 pKa = 3.77VFNLDD948 pKa = 3.39TDD950 pKa = 3.86NLEE953 pKa = 4.04EE954 pKa = 4.03RR955 pKa = 11.84TLTSEE960 pKa = 5.0VLTLDD965 pKa = 4.64DD966 pKa = 4.74GSTTILRR973 pKa = 11.84TVSGSEE979 pKa = 4.02FTWSDD984 pKa = 2.77KK985 pKa = 11.25SGWYY989 pKa = 8.84MPLQEE994 pKa = 5.2GSTASQDD1001 pKa = 2.97GGEE1004 pKa = 4.15RR1005 pKa = 11.84VITRR1009 pKa = 11.84PEE1011 pKa = 3.77LQNYY1015 pKa = 8.12VLFFNTLIPTGQVCAAGGYY1034 pKa = 8.08GWLMSVDD1041 pKa = 3.67VWTGLAPEE1049 pKa = 5.08DD1050 pKa = 4.81SVFDD1054 pKa = 4.32ANNDD1058 pKa = 3.54GVIDD1062 pKa = 4.14EE1063 pKa = 5.38DD1064 pKa = 4.25DD1065 pKa = 3.5YY1066 pKa = 11.84GYY1068 pKa = 10.87VGEE1071 pKa = 4.68MLSEE1075 pKa = 4.27SSPNEE1080 pKa = 3.84SGFLGTKK1087 pKa = 9.83QYY1089 pKa = 10.94TGTSDD1094 pKa = 4.17GEE1096 pKa = 4.19VHH1098 pKa = 5.95EE1099 pKa = 5.03RR1100 pKa = 11.84EE1101 pKa = 4.19VDD1103 pKa = 3.94FGDD1106 pKa = 3.65GDD1108 pKa = 3.68RR1109 pKa = 11.84AGRR1112 pKa = 11.84LSWEE1116 pKa = 4.41EE1117 pKa = 3.44ITPNN1121 pKa = 3.5

Molecular weight: 119.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1JY68|A0A2S1JY68_9GAMM Sulfurtransferase TusA family protein OS=Microbulbifer sp. A4B17 OX=359370 GN=BTJ40_03770 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27NGRR28 pKa = 11.84KK29 pKa = 8.87ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4240

0

4240

1368767

25

5896

322.8

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.873 ± 0.052

1.058 ± 0.016

5.661 ± 0.054

6.601 ± 0.041

4.008 ± 0.028

7.595 ± 0.043

2.07 ± 0.022

5.816 ± 0.031

4.415 ± 0.043

10.416 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.022

3.94 ± 0.03

4.292 ± 0.031

4.176 ± 0.03

5.491 ± 0.045

6.966 ± 0.05

5.089 ± 0.049

6.742 ± 0.033

1.394 ± 0.019

3.146 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski