Mycoplasma amphoriforme A39
Average proteome isoelectric point is 7.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 715 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A292IHZ5|A0A292IHZ5_9MOLU Uncharacterized protein OS=Mycoplasma amphoriforme A39 OX=572419 GN=MAMA39_01700 PE=3 SV=1
MM1 pKa = 7.33 AHH3 pKa = 6.59 SNNPAVKK10 pKa = 10.25 NNNQGYY16 pKa = 10.41 DD17 pKa = 3.51 GADD20 pKa = 3.25 QQNNEE25 pKa = 4.27 AVFNDD30 pKa = 3.34 LWGDD34 pKa = 3.54 SDD36 pKa = 5.28 QDD38 pKa = 4.26 LEE40 pKa = 4.62 LDD42 pKa = 3.82 NSGEE46 pKa = 4.0 QADD49 pKa = 4.51 EE50 pKa = 4.09 EE51 pKa = 4.7 FEE53 pKa = 4.3 LPEE56 pKa = 5.68 DD57 pKa = 3.92 GSIQIAYY64 pKa = 9.74 DD65 pKa = 3.43 ADD67 pKa = 3.71 GNGYY71 pKa = 9.43 YY72 pKa = 9.67 ISYY75 pKa = 10.27 DD76 pKa = 3.77 YY77 pKa = 9.63 EE78 pKa = 3.96 TGEE81 pKa = 4.42 YY82 pKa = 9.11 QDD84 pKa = 3.79 PWSGDD89 pKa = 3.42 VYY91 pKa = 10.96 DD92 pKa = 4.95 ISALFDD98 pKa = 3.44 EE99 pKa = 4.87 QGNPFSFFSDD109 pKa = 3.63 EE110 pKa = 4.19 PNEE113 pKa = 4.08 EE114 pKa = 4.2 VVSEE118 pKa = 4.42 EE119 pKa = 4.01 EE120 pKa = 4.21 QEE122 pKa = 4.24 ASPEE126 pKa = 3.75 QAYY129 pKa = 9.21 EE130 pKa = 4.02 VQAQQGAEE138 pKa = 3.87 QVYY141 pKa = 10.49 DD142 pKa = 4.38 EE143 pKa = 5.03 PAVIDD148 pKa = 3.83 DD149 pKa = 4.11 EE150 pKa = 5.0 VYY152 pKa = 10.47 EE153 pKa = 4.3 RR154 pKa = 11.84 QTHH157 pKa = 6.02 DD158 pKa = 4.19 LEE160 pKa = 5.13 KK161 pKa = 10.51 LQQEE165 pKa = 4.25 QSVTNQGASYY175 pKa = 9.3 QQQHH179 pKa = 6.36 DD180 pKa = 4.17 VPSEE184 pKa = 4.18 SNASIEE190 pKa = 4.36 QEE192 pKa = 4.35 SEE194 pKa = 3.87 QQLPPVQEE202 pKa = 4.37 TTSLQADD209 pKa = 3.99 EE210 pKa = 4.89 TLVTDD215 pKa = 4.12 PTLDD219 pKa = 4.84 DD220 pKa = 4.47 YY221 pKa = 11.02 YY222 pKa = 11.25 QHH224 pKa = 6.61 YY225 pKa = 9.6 NQVPYY230 pKa = 10.34 RR231 pKa = 11.84 YY232 pKa = 9.35 LDD234 pKa = 3.24 QYY236 pKa = 10.3 EE237 pKa = 3.97 QDD239 pKa = 3.5 ATEE242 pKa = 3.8 QALPVVDD249 pKa = 5.34 HH250 pKa = 5.88 VTSDD254 pKa = 3.55 PSQFEE259 pKa = 4.44 DD260 pKa = 4.43 GEE262 pKa = 4.21 QLITGLIGNNGYY274 pKa = 9.75 QPDD277 pKa = 3.81 NKK279 pKa = 9.69 TFEE282 pKa = 4.43 INAEE286 pKa = 3.81 EE287 pKa = 4.24 STAVTEE293 pKa = 5.01 LEE295 pKa = 3.94 PLVINGIEE303 pKa = 4.06 NSQQEE308 pKa = 4.31 AAVSQDD314 pKa = 3.23 QIGLSRR320 pKa = 11.84 TDD322 pKa = 3.59 EE323 pKa = 4.24 IKK325 pKa = 10.5 PDD327 pKa = 3.9 LVTIPQINEE336 pKa = 3.84 STSAPSQPEE345 pKa = 4.35 SNVSVQTEE353 pKa = 4.42 RR354 pKa = 11.84 YY355 pKa = 7.64 STVEE359 pKa = 4.02 PVSDD363 pKa = 3.72 AMVKK367 pKa = 10.19 AYY369 pKa = 9.02 YY370 pKa = 9.15 QQYY373 pKa = 10.65 SEE375 pKa = 4.8 IASQSHH381 pKa = 5.81 QPTQPQEE388 pKa = 3.87 QVVDD392 pKa = 3.86 SKK394 pKa = 11.68 PNVHH398 pKa = 6.87 YY399 pKa = 10.7 IPISAIDD406 pKa = 3.38 SSYY409 pKa = 10.94 QQDD412 pKa = 3.77 FDD414 pKa = 4.2 PQNDD418 pKa = 3.39 FHH420 pKa = 7.41 KK421 pKa = 10.95 FCDD424 pKa = 3.84 EE425 pKa = 3.79 QVTAQLHH432 pKa = 4.59 PTALVVDD439 pKa = 4.86 EE440 pKa = 5.12 SNSEE444 pKa = 4.09 PEE446 pKa = 4.07 DD447 pKa = 3.72 EE448 pKa = 4.81 KK449 pKa = 11.15 PVAVSPVDD457 pKa = 3.11 IDD459 pKa = 3.66 LAKK462 pKa = 10.86 SHH464 pKa = 7.44 DD465 pKa = 3.94 EE466 pKa = 4.11 SSDD469 pKa = 3.53 DD470 pKa = 3.5 FVLVNEE476 pKa = 4.53 PSDD479 pKa = 3.77 TKK481 pKa = 11.19 EE482 pKa = 3.85 LFAKK486 pKa = 10.43 KK487 pKa = 9.79 SVPEE491 pKa = 3.9 NYY493 pKa = 9.76 VFEE496 pKa = 4.55 NAHH499 pKa = 5.35 TQHH502 pKa = 6.1 ITPNLVYY509 pKa = 9.98 QAEE512 pKa = 4.47 SNEE515 pKa = 4.04 PSRR518 pKa = 11.84 ASQDD522 pKa = 3.43 DD523 pKa = 4.59 DD524 pKa = 3.91 IVDD527 pKa = 4.11 SKK529 pKa = 9.08 TQPIAIVDD537 pKa = 3.45 HH538 pKa = 6.5 SEE540 pKa = 4.51 NYY542 pKa = 8.83 VQPDD546 pKa = 3.39 SFLAPISHH554 pKa = 6.59 EE555 pKa = 3.62 PMLNNEE561 pKa = 4.1 NFSYY565 pKa = 10.12 TSEE568 pKa = 4.31 VADD571 pKa = 3.5 QLALPEE577 pKa = 4.36 IVPEE581 pKa = 4.49 IISATEE587 pKa = 4.02 SVSATTAFEE596 pKa = 4.14 FANDD600 pKa = 3.45 SSKK603 pKa = 9.23 QTSQEE608 pKa = 4.1 EE609 pKa = 4.74 VSVEE613 pKa = 3.95 PPVSQVEE620 pKa = 4.34 PLTVDD625 pKa = 4.82 DD626 pKa = 4.9 IFVPLKK632 pKa = 10.49 PEE634 pKa = 3.89 MEE636 pKa = 4.29 FVPDD640 pKa = 4.32 KK641 pKa = 11.14 VIEE644 pKa = 4.68 DD645 pKa = 3.59 DD646 pKa = 4.95 HH647 pKa = 8.55 GCDD650 pKa = 3.8 DD651 pKa = 4.1 CHH653 pKa = 7.19 DD654 pKa = 3.73 AHH656 pKa = 6.19 EE657 pKa = 4.82 TIALFDD663 pKa = 3.85 EE664 pKa = 4.95 KK665 pKa = 10.59 KK666 pKa = 10.86 DD667 pKa = 3.5 PVEE670 pKa = 5.14 AVDD673 pKa = 3.88 TMPKK677 pKa = 9.41 TEE679 pKa = 4.16 TNEE682 pKa = 4.0 PEE684 pKa = 3.98 EE685 pKa = 4.17 QQVDD689 pKa = 3.74 EE690 pKa = 5.64 AFIPLNFDD698 pKa = 3.21 HH699 pKa = 6.88 QNLVEE704 pKa = 5.87 DD705 pKa = 5.17 DD706 pKa = 3.97 NQLDD710 pKa = 3.89 FTIHH714 pKa = 7.35 AEE716 pKa = 4.07 EE717 pKa = 4.63 QPTVDD722 pKa = 2.71 QFVIVEE728 pKa = 4.24 EE729 pKa = 4.24 NGALQASFATTFAKK743 pKa = 10.36 KK744 pKa = 10.27 DD745 pKa = 3.66 VVCNEE750 pKa = 4.03 PEE752 pKa = 4.26 GMSFTVVDD760 pKa = 3.81 EE761 pKa = 4.52 LEE763 pKa = 4.06 PVVFVKK769 pKa = 10.4 PADD772 pKa = 4.63 RR773 pKa = 11.84 IVQPEE778 pKa = 4.09 KK779 pKa = 10.89 PKK781 pKa = 10.77 DD782 pKa = 3.57 LEE784 pKa = 4.1 LAPVEE789 pKa = 4.26 FSEE792 pKa = 5.5 PSIDD796 pKa = 3.83 DD797 pKa = 3.49 VKK799 pKa = 11.42 VEE801 pKa = 4.45 PEE803 pKa = 3.81 PVTWPEE809 pKa = 3.58 ITPVITDD816 pKa = 3.32 VPNSFEE822 pKa = 4.84 SNQSLPWYY830 pKa = 10.0 AAVGSNASEE839 pKa = 4.38 CDD841 pKa = 3.41 QKK843 pKa = 9.84 THH845 pKa = 6.97 ADD847 pKa = 3.49 HH848 pKa = 7.02 FNEE851 pKa = 3.86 VLLNEE856 pKa = 4.52 SLAEE860 pKa = 4.02 VQKK863 pKa = 9.65 TDD865 pKa = 3.76 EE866 pKa = 4.21 IMEE869 pKa = 4.5 VPQSTPSANKK879 pKa = 8.84 QDD881 pKa = 2.92 ISIFEE886 pKa = 4.32 NYY888 pKa = 8.64 GKK890 pKa = 9.92 PEE892 pKa = 3.53 ITIVNDD898 pKa = 3.94 EE899 pKa = 4.06 ISATPSHH906 pKa = 6.35 SLFGKK911 pKa = 9.65 SEE913 pKa = 4.19 QPVEE917 pKa = 4.12 PYY919 pKa = 11.01 LPFTITNDD927 pKa = 2.87 SDD929 pKa = 5.07 LVVQFNKK936 pKa = 10.2 KK937 pKa = 9.68 PSLSGFKK944 pKa = 10.06 FDD946 pKa = 5.44 SIQLEE951 pKa = 4.29 EE952 pKa = 4.25 QQVSPDD958 pKa = 2.96 AHH960 pKa = 6.97 LEE962 pKa = 3.85 PDD964 pKa = 3.5 EE965 pKa = 4.95 AVAPVPSVVLEE976 pKa = 4.01 PEE978 pKa = 3.84 QTKK981 pKa = 10.45 EE982 pKa = 3.66 IEE984 pKa = 4.01 PAPKK988 pKa = 9.27 IKK990 pKa = 10.29 KK991 pKa = 9.94 AFWEE995 pKa = 4.53 SYY997 pKa = 9.91 IGNFDD1002 pKa = 3.65 YY1003 pKa = 11.3 GHH1005 pKa = 7.09 FDD1007 pKa = 5.77 DD1008 pKa = 5.49 DD1009 pKa = 5.7 NKK1011 pKa = 10.41 WVWDD1015 pKa = 3.65 GFFYY1019 pKa = 11.06 EE1020 pKa = 4.47 NGTFVSSRR1028 pKa = 11.84 TAPPIAPDD1036 pKa = 4.31 PKK1038 pKa = 10.94 PMTALNQQEE1047 pKa = 4.67 SNPTLVTDD1055 pKa = 3.74 FLIKK1059 pKa = 10.21 KK1060 pKa = 8.95 RR1061 pKa = 11.84 EE1062 pKa = 3.9 EE1063 pKa = 3.81 VMVPVRR1069 pKa = 11.84 PVQTQAEE1076 pKa = 4.86 LYY1078 pKa = 10.46 GNPEE1082 pKa = 4.01 YY1083 pKa = 10.47 DD1084 pKa = 3.33 TDD1086 pKa = 4.52 VVMLDD1091 pKa = 5.17 DD1092 pKa = 5.05 EE1093 pKa = 4.89 GTSQALLRR1101 pKa = 11.84 ANKK1104 pKa = 9.63 AHH1106 pKa = 6.45 EE1107 pKa = 5.12 AILRR1111 pKa = 11.84 PKK1113 pKa = 9.91 PAVEE1117 pKa = 4.16 DD1118 pKa = 3.49 KK1119 pKa = 11.22 KK1120 pKa = 10.93 PVIEE1124 pKa = 5.22 IDD1126 pKa = 4.55 DD1127 pKa = 3.69 IQEE1130 pKa = 4.01 VEE1132 pKa = 4.16 NEE1134 pKa = 4.18 EE1135 pKa = 4.1 IVPKK1139 pKa = 9.01 VTKK1142 pKa = 10.21 TEE1144 pKa = 4.39 TILEE1148 pKa = 4.25 PKK1150 pKa = 8.55 MQPKK1154 pKa = 10.0 KK1155 pKa = 11.01 FNFLTNRR1162 pKa = 11.84 FTPPSEE1168 pKa = 4.09 NLDD1171 pKa = 3.59 ALTTEE1176 pKa = 4.49 IVSGEE1181 pKa = 4.04 PSCLSEE1187 pKa = 5.8 DD1188 pKa = 4.06 DD1189 pKa = 5.06 LFASTTYY1196 pKa = 10.87 DD1197 pKa = 3.27 NKK1199 pKa = 10.6 IKK1201 pKa = 9.52 EE1202 pKa = 4.57 TLLNMVGFLNKK1213 pKa = 9.98 ASAEE1217 pKa = 3.97 ATHH1220 pKa = 6.31 VYY1222 pKa = 8.58 KK1223 pKa = 10.75 TIAKK1227 pKa = 8.64 EE1228 pKa = 3.69 LKK1230 pKa = 10.13 IEE1232 pKa = 4.09 IKK1234 pKa = 10.5 NIKK1237 pKa = 8.83 QDD1239 pKa = 3.61 NEE1241 pKa = 3.88 KK1242 pKa = 10.79 LRR1244 pKa = 11.84 LEE1246 pKa = 4.22 FKK1248 pKa = 10.55 EE1249 pKa = 3.76 NEE1251 pKa = 3.77 NRR1253 pKa = 11.84 ISAKK1257 pKa = 10.45 KK1258 pKa = 10.1 SDD1260 pKa = 4.17 LLKK1263 pKa = 10.32 TITSDD1268 pKa = 4.11 FKK1270 pKa = 11.16 QGTNFNTGKK1279 pKa = 10.37 DD1280 pKa = 3.75 FDD1282 pKa = 4.59 SLQHH1286 pKa = 5.43 AHH1288 pKa = 7.72 DD1289 pKa = 4.36 YY1290 pKa = 10.81 NKK1292 pKa = 10.58 HH1293 pKa = 5.76 LNEE1296 pKa = 3.81 TMKK1299 pKa = 11.04 ANEE1302 pKa = 4.11 EE1303 pKa = 3.94 RR1304 pKa = 11.84 LQVLTNNQVQLKK1316 pKa = 9.23 IVYY1319 pKa = 9.3 DD1320 pKa = 3.55 RR1321 pKa = 11.84 RR1322 pKa = 11.84 MSKK1325 pKa = 10.27 LASDD1329 pKa = 4.39 ANRR1332 pKa = 11.84 LEE1334 pKa = 4.15 NLLSGADD1341 pKa = 3.87 YY1342 pKa = 10.58 RR1343 pKa = 11.84 DD1344 pKa = 3.67 HH1345 pKa = 7.55 SLDD1348 pKa = 3.63 MIEE1351 pKa = 3.94 EE1352 pKa = 4.26 NYY1354 pKa = 10.72 RR1355 pKa = 11.84 NLLKK1359 pKa = 11.01 AQDD1362 pKa = 3.94 DD1363 pKa = 3.83 YY1364 pKa = 12.02 RR1365 pKa = 11.84 NRR1367 pKa = 11.84 LNHH1370 pKa = 6.2 FVPTTFPNLKK1380 pKa = 9.77 NQMLPSTGLFQNDD1393 pKa = 3.02 FNEE1396 pKa = 4.28 FSDD1399 pKa = 5.6 LSALDD1404 pKa = 3.23 ATFYY1408 pKa = 11.39 SKK1410 pKa = 11.01 LATPSFYY1417 pKa = 11.3 NNNLPLPFEE1426 pKa = 4.2 TQPRR1430 pKa = 11.84 LLDD1433 pKa = 3.8 NSHH1436 pKa = 6.54 SLLGNLDD1443 pKa = 3.15 NSYY1446 pKa = 11.65 DD1447 pKa = 3.8 LFSEE1451 pKa = 4.38 KK1452 pKa = 11.03 SNFTSDD1458 pKa = 3.27 LLKK1461 pKa = 10.63 PFNPQGQDD1469 pKa = 2.74 SLKK1472 pKa = 11.32 AMDD1475 pKa = 4.51 RR1476 pKa = 11.84 YY1477 pKa = 10.42 FNRR1480 pKa = 11.84 DD1481 pKa = 3.25 FEE1483 pKa = 4.55 LLKK1486 pKa = 10.36 PDD1488 pKa = 3.54 RR1489 pKa = 11.84 TTSNLDD1495 pKa = 3.57 DD1496 pKa = 5.14 LFSDD1500 pKa = 4.69 DD1501 pKa = 5.82 DD1502 pKa = 4.29 ALFSDD1507 pKa = 5.17 KK1508 pKa = 11.03 YY1509 pKa = 10.76 DD1510 pKa = 4.0 ANASLTDD1517 pKa = 3.56 EE1518 pKa = 5.48 LEE1520 pKa = 4.35 DD1521 pKa = 3.99 LLRR1524 pKa = 11.84 RR1525 pKa = 4.0
Molecular weight: 172.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.211
IPC2_protein 4.05
IPC_protein 4.062
Toseland 3.872
ProMoST 4.177
Dawson 4.024
Bjellqvist 4.164
Wikipedia 3.91
Rodwell 3.884
Grimsley 3.77
Solomon 4.024
Lehninger 3.973
Nozaki 4.126
DTASelect 4.317
Thurlkill 3.884
EMBOSS 3.923
Sillero 4.177
Patrickios 3.503
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.089
Protein with the highest isoelectric point:
>tr|A0A292IJ84|A0A292IJ84_9MOLU Uncharacterized protein OS=Mycoplasma amphoriforme A39 OX=572419 GN=MAMA39_06280 PE=4 SV=1
MM1 pKa = 7.52 KK2 pKa = 9.71 PHH4 pKa = 7.48 LSTFSTVQWYY14 pKa = 9.58 KK15 pKa = 10.91 CRR17 pKa = 11.84 GTRR20 pKa = 11.84 KK21 pKa = 10.11 SMYY24 pKa = 10.36 LNLFVFRR31 pKa = 11.84 SLSQSCNCDD40 pKa = 3.15 NNRR43 pKa = 11.84 LNLSASQVSSTKK55 pKa = 9.88 IQANEE60 pKa = 3.66 LSRR63 pKa = 11.84 MKK65 pKa = 10.78 NRR67 pKa = 11.84 LLPSDD72 pKa = 4.24 SRR74 pKa = 11.84 CSDD77 pKa = 2.97 GKK79 pKa = 10.78 RR80 pKa = 11.84 FLRR83 pKa = 11.84 LISSIFLFRR92 pKa = 11.84 PSIFRR97 pKa = 11.84 SKK99 pKa = 10.49 YY100 pKa = 8.18 CSKK103 pKa = 10.7 RR104 pKa = 11.84 SNFSLTSAWSTWILSKK120 pKa = 11.09 ASLIFFLNFASTSSIFLYY138 pKa = 10.42 NKK140 pKa = 9.25 SIRR143 pKa = 11.84 WFKK146 pKa = 10.78 RR147 pKa = 11.84 FSTLVIILLISSILALVLFNSSRR170 pKa = 11.84 TIFAYY175 pKa = 9.88 SGKK178 pKa = 10.66 GIIFSLSINTKK189 pKa = 6.97 YY190 pKa = 10.25 EE191 pKa = 3.71 RR192 pKa = 11.84 SGLIFFKK199 pKa = 11.13 VLLFCIDD206 pKa = 3.49 SYY208 pKa = 11.48 LIIIFFGDD216 pKa = 3.53 KK217 pKa = 10.37 YY218 pKa = 11.47 NKK220 pKa = 10.36 LSIDD224 pKa = 3.68 KK225 pKa = 10.57 IKK227 pKa = 10.79 LKK229 pKa = 10.65 KK230 pKa = 10.22
Molecular weight: 26.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.853
IPC_protein 10.292
Toseland 10.701
ProMoST 10.306
Dawson 10.804
Bjellqvist 10.452
Wikipedia 10.965
Rodwell 11.272
Grimsley 10.847
Solomon 10.862
Lehninger 10.833
Nozaki 10.687
DTASelect 10.452
Thurlkill 10.701
EMBOSS 11.082
Sillero 10.73
Patrickios 10.979
IPC_peptide 10.862
IPC2_peptide 9.414
IPC2.peptide.svr19 8.505
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
715
0
715
274971
37
1971
384.6
43.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.768 ± 0.082
0.717 ± 0.023
5.307 ± 0.076
5.716 ± 0.127
5.105 ± 0.086
4.921 ± 0.106
1.959 ± 0.034
8.503 ± 0.098
9.221 ± 0.108
9.589 ± 0.096
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.773 ± 0.034
7.419 ± 0.088
3.452 ± 0.078
4.54 ± 0.092
3.331 ± 0.058
6.446 ± 0.064
5.557 ± 0.063
5.942 ± 0.074
0.985 ± 0.032
3.751 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here