Reinekea thalattae
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2651 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C8Z5F9|A0A5C8Z5F9_9GAMM VOC family protein OS=Reinekea thalattae OX=2593301 GN=FME95_01110 PE=4 SV=1
MM1 pKa = 8.42 KK2 pKa = 10.04 MMSVWVSALLALFVSACTSVNDD24 pKa = 4.07 DD25 pKa = 3.95 DD26 pKa = 4.82 KK27 pKa = 11.93 VVIGVATDD35 pKa = 3.35 AEE37 pKa = 4.49 KK38 pKa = 10.62 QVLIHH43 pKa = 5.76 YY44 pKa = 7.29 LPWFADD50 pKa = 3.31 EE51 pKa = 5.83 NYY53 pKa = 9.28 AQEE56 pKa = 4.37 PSRR59 pKa = 11.84 HH60 pKa = 5.04 WSHH63 pKa = 5.58 GTVNEE68 pKa = 4.13 ALVGEE73 pKa = 4.56 YY74 pKa = 10.33 DD75 pKa = 3.75 SQSWATQLYY84 pKa = 10.11 HH85 pKa = 6.95 ILLSAAVGVDD95 pKa = 2.78 GAMINIRR102 pKa = 11.84 TDD104 pKa = 3.19 YY105 pKa = 11.38 DD106 pKa = 3.5 KK107 pKa = 11.47 DD108 pKa = 3.44 ALSIFMEE115 pKa = 4.08 SLEE118 pKa = 4.21 RR119 pKa = 11.84 VRR121 pKa = 11.84 AVYY124 pKa = 10.21 PDD126 pKa = 3.87 FAYY129 pKa = 10.45 HH130 pKa = 6.53 ISASIDD136 pKa = 3.56 DD137 pKa = 3.86 QDD139 pKa = 3.58 KK140 pKa = 10.7 TEE142 pKa = 4.89 ALAQQEE148 pKa = 4.28 FEE150 pKa = 4.81 YY151 pKa = 10.55 IAEE154 pKa = 4.94 LIDD157 pKa = 4.17 NYY159 pKa = 8.15 TTYY162 pKa = 10.88 LHH164 pKa = 7.04 KK165 pKa = 10.76 NGKK168 pKa = 8.44 PVIFIWAYY176 pKa = 11.28 NNLTPAQYY184 pKa = 10.64 RR185 pKa = 11.84 SIADD189 pKa = 3.27 QVFGEE194 pKa = 4.64 DD195 pKa = 4.29 EE196 pKa = 4.57 VILVQNDD203 pKa = 2.72 IDD205 pKa = 3.92 TSAVADD211 pKa = 4.99 EE212 pKa = 4.5 INMNAFYY219 pKa = 9.83 PWVKK223 pKa = 10.51 GFADD227 pKa = 5.07 DD228 pKa = 4.21 GSDD231 pKa = 3.03 WGEE234 pKa = 4.17 VYY236 pKa = 10.95 LDD238 pKa = 3.07 WFYY241 pKa = 10.99 RR242 pKa = 11.84 TSADD246 pKa = 3.41 YY247 pKa = 9.26 LTDD250 pKa = 3.41 NKK252 pKa = 10.72 QEE254 pKa = 4.37 FIVAGVWPGFDD265 pKa = 3.23 DD266 pKa = 5.47 RR267 pKa = 11.84 QVNWYY272 pKa = 9.76 GDD274 pKa = 3.76 DD275 pKa = 3.72 YY276 pKa = 11.39 AGRR279 pKa = 11.84 WIDD282 pKa = 3.54 RR283 pKa = 11.84 ADD285 pKa = 3.22 GAIYY289 pKa = 10.61 DD290 pKa = 4.26 NTWQLIDD297 pKa = 3.59 TFNNEE302 pKa = 3.63 WQQDD306 pKa = 3.25 IATEE310 pKa = 4.8 DD311 pKa = 3.39 ISIDD315 pKa = 3.19 WVVIEE320 pKa = 4.35 TWNDD324 pKa = 3.13 FNEE327 pKa = 4.58 GSEE330 pKa = 4.22 IEE332 pKa = 4.91 PIAATADD339 pKa = 3.76 TEE341 pKa = 4.44 SFQYY345 pKa = 11.05 LKK347 pKa = 10.26 LTDD350 pKa = 3.73 SYY352 pKa = 10.42 ITNFKK357 pKa = 9.74 ATDD360 pKa = 3.65 SVLDD364 pKa = 4.43 ADD366 pKa = 5.93 DD367 pKa = 4.59 EE368 pKa = 4.55 LLNAAVKK375 pKa = 10.1 IYY377 pKa = 10.25 QAAKK381 pKa = 10.5 LIDD384 pKa = 4.37 DD385 pKa = 4.79 GDD387 pKa = 3.94 RR388 pKa = 11.84 ASANYY393 pKa = 10.41 YY394 pKa = 8.7 STLEE398 pKa = 4.06 QAIEE402 pKa = 4.31 AYY404 pKa = 10.4 LKK406 pKa = 10.8 ADD408 pKa = 3.5 AEE410 pKa = 4.52 GASDD414 pKa = 3.88 YY415 pKa = 11.59 AEE417 pKa = 4.38 TIIDD421 pKa = 3.74 GG422 pKa = 3.98
Molecular weight: 47.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.516
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.961
Patrickios 1.176
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A5C8ZBJ2|A0A5C8ZBJ2_9GAMM MATE family efflux transporter OS=Reinekea thalattae OX=2593301 GN=FME95_09855 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 8.92 RR14 pKa = 11.84 SHH16 pKa = 6.09 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.46 NGRR28 pKa = 11.84 KK29 pKa = 8.96 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 AALSAA44 pKa = 4.04
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2651
0
2651
861729
18
2531
325.1
36.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.235 ± 0.047
0.954 ± 0.015
5.662 ± 0.043
6.357 ± 0.045
4.043 ± 0.036
6.574 ± 0.04
2.113 ± 0.024
6.409 ± 0.039
4.792 ± 0.042
10.705 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.422 ± 0.025
4.076 ± 0.033
3.832 ± 0.031
5.042 ± 0.041
4.572 ± 0.036
6.932 ± 0.045
5.191 ± 0.039
6.832 ± 0.038
1.248 ± 0.018
3.009 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here