Oceaniovalibus guishaninsula JLT2003
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2840 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K2HF72|K2HF72_9RHOB PINc domain-containing protein OS=Oceaniovalibus guishaninsula JLT2003 OX=1231392 GN=OCGS_0817 PE=4 SV=1
MM1 pKa = 7.23 QRR3 pKa = 11.84 TLYY6 pKa = 11.03 ASVAALALGTGFAAAQDD23 pKa = 4.45 LIFPVGEE30 pKa = 4.46 GAFNWDD36 pKa = 3.7 SYY38 pKa = 8.88 QQFSDD43 pKa = 3.43 ATDD46 pKa = 3.85 LSGQTLDD53 pKa = 4.0 ITGPWTGEE61 pKa = 3.96 DD62 pKa = 3.23 NDD64 pKa = 4.84 LFTSVIAYY72 pKa = 9.97 FEE74 pKa = 4.25 EE75 pKa = 4.36 ATGAEE80 pKa = 4.38 VNYY83 pKa = 10.53 SGSDD87 pKa = 3.18 SFEE90 pKa = 3.74 QDD92 pKa = 2.43 IVISTRR98 pKa = 11.84 SNSAPNIAVIPQPGLVGDD116 pKa = 4.8 LASEE120 pKa = 5.5 GYY122 pKa = 7.81 LTPLAPEE129 pKa = 3.94 TTDD132 pKa = 3.4 WIVEE136 pKa = 4.13 NYY138 pKa = 10.47 AAGQSWADD146 pKa = 3.36 LATFEE151 pKa = 4.68 GQDD154 pKa = 3.6 GQTQLYY160 pKa = 8.98 AFPYY164 pKa = 9.67 KK165 pKa = 9.85 IDD167 pKa = 3.59 VKK169 pKa = 10.85 SLVWYY174 pKa = 10.29 VPEE177 pKa = 3.88 QFEE180 pKa = 4.17 EE181 pKa = 3.99 AGYY184 pKa = 10.35 DD185 pKa = 3.5 VPEE188 pKa = 4.18 TLEE191 pKa = 4.0 EE192 pKa = 3.99 LKK194 pKa = 10.67 QLNDD198 pKa = 3.44 QIVADD203 pKa = 5.65 GGTPWCIGLGSGAATGWPATDD224 pKa = 3.12 WVEE227 pKa = 5.76 DD228 pKa = 3.28 MMLRR232 pKa = 11.84 INPPEE237 pKa = 5.89 IYY239 pKa = 10.11 DD240 pKa = 3.3 QWVSNDD246 pKa = 3.31 IPFDD250 pKa = 4.19 DD251 pKa = 4.67 PQVVAAIEE259 pKa = 4.49 EE260 pKa = 4.36 YY261 pKa = 11.18 GQFARR266 pKa = 11.84 NDD268 pKa = 3.59 NYY270 pKa = 10.77 VSGGSSSVANTDD282 pKa = 4.16 FRR284 pKa = 11.84 DD285 pKa = 3.94 APAGLFEE292 pKa = 5.6 FPPQCYY298 pKa = 8.5 MLKK301 pKa = 9.78 QATFIPTFFPEE312 pKa = 4.29 GTEE315 pKa = 3.84 VGTDD319 pKa = 2.69 VDD321 pKa = 4.82 FFYY324 pKa = 11.12 FPASAEE330 pKa = 4.04 KK331 pKa = 10.83 DD332 pKa = 3.03 LGKK335 pKa = 9.73 PVLGAGTMFTITKK348 pKa = 10.46 DD349 pKa = 3.0 SDD351 pKa = 3.23 AAQAFIEE358 pKa = 4.34 FLKK361 pKa = 10.39 TPIAHH366 pKa = 6.39 EE367 pKa = 3.85 LWMAQSGFLTPYY379 pKa = 10.24 KK380 pKa = 10.43 DD381 pKa = 3.45 ANTDD385 pKa = 3.47 VFPSDD390 pKa = 3.35 SQRR393 pKa = 11.84 NMNDD397 pKa = 2.5 ILTSATTFRR406 pKa = 11.84 FDD408 pKa = 4.84 GSDD411 pKa = 3.52 LMPSEE416 pKa = 4.44 IGAGAFWTGMVDD428 pKa = 3.45 YY429 pKa = 7.62 TTGADD434 pKa = 3.49 AQTVASQIQSRR445 pKa = 11.84 WEE447 pKa = 3.63 QLKK450 pKa = 10.76
Molecular weight: 49.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.63
IPC_protein 3.668
Toseland 3.439
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.35
Solomon 3.643
Lehninger 3.605
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.49
EMBOSS 3.592
Sillero 3.783
Patrickios 1.837
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.713
Protein with the highest isoelectric point:
>tr|K2HN72|K2HN72_9RHOB Pyrophosphate phospho-hydrolase OS=Oceaniovalibus guishaninsula JLT2003 OX=1231392 GN=OCGS_1820 PE=3 SV=1
MM1 pKa = 7.51 TKK3 pKa = 9.12 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 KK14 pKa = 8.83 HH15 pKa = 4.64 RR16 pKa = 11.84 HH17 pKa = 3.91 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.37 AGRR29 pKa = 11.84 KK30 pKa = 8.54 ILNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.03 GRR40 pKa = 11.84 KK41 pKa = 8.43 SLCAA45 pKa = 3.75
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.379
IPC2_protein 10.818
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.31
Grimsley 12.603
Solomon 13.042
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.047
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.061
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2840
0
2840
883308
37
1797
311.0
33.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.666 ± 0.075
0.852 ± 0.015
6.808 ± 0.041
5.022 ± 0.046
3.491 ± 0.031
9.172 ± 0.043
1.974 ± 0.025
4.808 ± 0.033
2.22 ± 0.034
10.145 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.706 ± 0.023
2.152 ± 0.021
5.519 ± 0.032
2.848 ± 0.026
8.009 ± 0.049
4.397 ± 0.031
5.337 ± 0.03
7.397 ± 0.04
1.453 ± 0.022
2.023 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here