Nyamanini virus (isolate Tick/Thailand/39/1968)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Nyamiviridae; Nyavirus; Nyamanini nyavirus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4NFM0|C4NFM0_NYMVT Nucleocapsid OS=Nyamanini virus (isolate Tick/Thailand/39/1968) OX=1557032 PE=4 SV=1
MM1 pKa = 7.81EE2 pKa = 5.38EE3 pKa = 4.77GKK5 pKa = 10.41DD6 pKa = 3.52NPPAGGSGDD15 pKa = 3.9AEE17 pKa = 4.11LEE19 pKa = 4.24KK20 pKa = 11.07ARR22 pKa = 11.84TEE24 pKa = 4.23YY25 pKa = 11.01LVTRR29 pKa = 11.84HH30 pKa = 6.55RR31 pKa = 11.84DD32 pKa = 3.02QCPQDD37 pKa = 3.34RR38 pKa = 11.84QPSHH42 pKa = 6.81EE43 pKa = 4.48EE44 pKa = 3.62DD45 pKa = 3.37TAEE48 pKa = 4.14EE49 pKa = 4.04TWEE52 pKa = 4.07VEE54 pKa = 4.28SYY56 pKa = 10.63LQEE59 pKa = 4.75GDD61 pKa = 4.04LEE63 pKa = 4.45TGEE66 pKa = 4.65RR67 pKa = 11.84PEE69 pKa = 5.75DD70 pKa = 4.03PDD72 pKa = 5.01LEE74 pKa = 4.41ATSSEE79 pKa = 4.25EE80 pKa = 4.2EE81 pKa = 3.64TWEE84 pKa = 3.91AQEE87 pKa = 4.03SSEE90 pKa = 4.21EE91 pKa = 4.28GEE93 pKa = 4.07EE94 pKa = 4.24EE95 pKa = 3.86EE96 pKa = 5.46GEE98 pKa = 4.13EE99 pKa = 4.12EE100 pKa = 4.46EE101 pKa = 4.33EE102 pKa = 4.64GEE104 pKa = 4.33EE105 pKa = 4.31RR106 pKa = 11.84GAAGPAEE113 pKa = 4.55DD114 pKa = 4.7ANLDD118 pKa = 3.23KK119 pKa = 11.04RR120 pKa = 11.84MQARR124 pKa = 11.84FVQWQSNTIRR134 pKa = 11.84LLRR137 pKa = 11.84SDD139 pKa = 3.34KK140 pKa = 11.3QEE142 pKa = 4.14DD143 pKa = 3.72KK144 pKa = 11.38EE145 pKa = 4.25EE146 pKa = 4.29GSRR149 pKa = 11.84AIALTYY155 pKa = 9.91RR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84EE160 pKa = 3.7QGVVEE165 pKa = 4.91FYY167 pKa = 10.47KK168 pKa = 10.33WRR170 pKa = 11.84SLLILEE176 pKa = 4.74SEE178 pKa = 4.84LARR181 pKa = 11.84AAPRR185 pKa = 11.84VLTLASQPTLEE196 pKa = 3.97ASKK199 pKa = 10.03WDD201 pKa = 3.68EE202 pKa = 4.6VLDD205 pKa = 4.05EE206 pKa = 4.35LQGLQIEE213 pKa = 4.68DD214 pKa = 3.37

Molecular weight: 24.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4NFL8|C4NFL8_NYMVT ORF III OS=Nyamanini virus (isolate Tick/Thailand/39/1968) OX=1557032 PE=4 SV=1
MM1 pKa = 7.64ALQVKK6 pKa = 10.0AKK8 pKa = 9.45EE9 pKa = 3.78ASIWYY14 pKa = 7.1QARR17 pKa = 11.84VKK19 pKa = 10.05TPTRR23 pKa = 11.84EE24 pKa = 3.96LVLSVPILKK33 pKa = 10.07VPRR36 pKa = 11.84KK37 pKa = 10.21GEE39 pKa = 3.76FSQRR43 pKa = 11.84PDD45 pKa = 3.05PSVFRR50 pKa = 11.84DD51 pKa = 3.47SKK53 pKa = 11.21ACIFIHH59 pKa = 6.64DD60 pKa = 4.49KK61 pKa = 11.14KK62 pKa = 10.65PFLRR66 pKa = 11.84VEE68 pKa = 4.49LDD70 pKa = 3.38PLRR73 pKa = 11.84HH74 pKa = 6.25KK75 pKa = 9.18KK76 pKa = 8.7TKK78 pKa = 10.45VSVPQSQVINLADD91 pKa = 4.5LGWTARR97 pKa = 11.84DD98 pKa = 3.9FAAHH102 pKa = 6.33PMSVRR107 pKa = 11.84YY108 pKa = 9.44EE109 pKa = 3.68RR110 pKa = 11.84VIFKK114 pKa = 9.32EE115 pKa = 4.1PCSPLLDD122 pKa = 3.7EE123 pKa = 5.24KK124 pKa = 11.27RR125 pKa = 11.84PFLFADD131 pKa = 3.85

Molecular weight: 15.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3691

131

1936

615.2

69.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.234 ± 0.222

2.086 ± 0.4

4.606 ± 0.399

8.67 ± 2.171

2.574 ± 0.322

5.662 ± 0.303

2.547 ± 0.349

4.01 ± 0.2

5.608 ± 1.255

12.246 ± 1.43

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.815 ± 0.163

2.791 ± 0.273

5.527 ± 0.338

4.226 ± 0.188

7.315 ± 0.914

7.884 ± 0.723

5.5 ± 0.534

5.093 ± 0.409

1.788 ± 0.297

2.818 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski