Wenzhou tombus-like virus 4
Average proteome isoelectric point is 7.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGC4|A0A1L3KGC4_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 4 OX=1923674 PE=4 SV=1
MM1 pKa = 7.1 NTTNYY6 pKa = 10.82 AGLSDD11 pKa = 5.1 LLTAGSRR18 pKa = 11.84 SIAYY22 pKa = 9.81 SKK24 pKa = 10.82 LLSAEE29 pKa = 4.0 AAGQLEE35 pKa = 4.71 PGAASYY41 pKa = 10.21 IISALDD47 pKa = 3.56 PFHH50 pKa = 7.59 DD51 pKa = 3.94 KK52 pKa = 10.96 QYY54 pKa = 11.04 DD55 pKa = 3.64 ATDD58 pKa = 3.62 VPSVGVQRR66 pKa = 11.84 SIVRR70 pKa = 11.84 VLRR73 pKa = 11.84 TRR75 pKa = 11.84 TRR77 pKa = 11.84 ISMEE81 pKa = 3.75 SATSVGYY88 pKa = 6.78 TQPGRR93 pKa = 11.84 LALCMYY99 pKa = 9.75 PSVNSRR105 pKa = 11.84 YY106 pKa = 8.69 MSKK109 pKa = 10.68 KK110 pKa = 10.61 DD111 pKa = 3.31 IVNGQAVDD119 pKa = 3.7 PATAAAKK126 pKa = 10.33 YY127 pKa = 10.3 VGGLVWEE134 pKa = 5.21 LDD136 pKa = 3.29 IQGQPKK142 pKa = 9.82 FFTGDD147 pKa = 3.39 PSDD150 pKa = 4.13 YY151 pKa = 11.12 SADD154 pKa = 3.66 ANNGRR159 pKa = 11.84 MSIADD164 pKa = 3.6 VTGGMPDD171 pKa = 3.43 HH172 pKa = 6.64 AHH174 pKa = 5.8 MRR176 pKa = 11.84 VIGCGFEE183 pKa = 3.94 VHH185 pKa = 6.25 NVTEE189 pKa = 4.29 EE190 pKa = 3.92 LSKK193 pKa = 11.34 GGTVSVWQLPGIFARR208 pKa = 11.84 NKK210 pKa = 9.7 VVDD213 pKa = 3.82 GTQVVTNAPATGIYY227 pKa = 9.43 YY228 pKa = 10.56 NCDD231 pKa = 3.13 FLGRR235 pKa = 11.84 APTTADD241 pKa = 3.11 EE242 pKa = 4.54 VTNIPTGQTWEE253 pKa = 4.22 AGQGCLVVPRR263 pKa = 11.84 LDD265 pKa = 3.81 SGDD268 pKa = 3.85 FLLAGCNTPNAEE280 pKa = 4.04 ALIEE284 pKa = 4.23 GVWDD288 pKa = 3.92 GNGTYY293 pKa = 10.2 AYY295 pKa = 10.02 FPTGSNTSVRR305 pKa = 11.84 TFSTPFSMGGALFEE319 pKa = 4.37 GLPPEE324 pKa = 4.25 SVLDD328 pKa = 4.3 VEE330 pKa = 5.3 CKK332 pKa = 9.84 WIVAEE337 pKa = 4.44 VPHH340 pKa = 7.47 VSDD343 pKa = 4.71 IGDD346 pKa = 3.53 LVIARR351 pKa = 11.84 NTPPGMFPHH360 pKa = 5.43 TTKK363 pKa = 11.13 LIACLNRR370 pKa = 11.84 TLRR373 pKa = 11.84 DD374 pKa = 3.85 GYY376 pKa = 9.36 PAGWNEE382 pKa = 3.89 SGKK385 pKa = 9.96 YY386 pKa = 7.15 WSSVLRR392 pKa = 11.84 AISSVLPMIGNAVPIPGAKK411 pKa = 10.12 LLTSAASAALQQKK424 pKa = 9.35 ARR426 pKa = 11.84 NRR428 pKa = 11.84 DD429 pKa = 3.29 EE430 pKa = 5.36 TIDD433 pKa = 3.74 LMKK436 pKa = 10.25 DD437 pKa = 3.43 LKK439 pKa = 10.55 SARR442 pKa = 11.84 PGTKK446 pKa = 9.29 EE447 pKa = 3.48 YY448 pKa = 11.47 NEE450 pKa = 5.05 LYY452 pKa = 9.59 RR453 pKa = 11.84 QAMANAGPIQRR464 pKa = 11.84 RR465 pKa = 11.84 QLRR468 pKa = 11.84 RR469 pKa = 11.84 LRR471 pKa = 11.84 RR472 pKa = 11.84 ADD474 pKa = 3.12 EE475 pKa = 4.07 RR476 pKa = 11.84 TVKK479 pKa = 10.23 GFTMVSPSTGSKK491 pKa = 9.08 TKK493 pKa = 10.85 LPTSSIAVVKK503 pKa = 10.58 RR504 pKa = 11.84 RR505 pKa = 3.48
Molecular weight: 54.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.265
IPC2_protein 7.644
IPC_protein 7.541
Toseland 7.176
ProMoST 8.2
Dawson 8.229
Bjellqvist 8.536
Wikipedia 8.156
Rodwell 8.244
Grimsley 7.19
Solomon 8.317
Lehninger 8.331
Nozaki 8.668
DTASelect 8.302
Thurlkill 8.346
EMBOSS 8.434
Sillero 8.653
Patrickios 4.342
IPC_peptide 8.317
IPC2_peptide 7.527
IPC2.peptide.svr19 7.717
Protein with the highest isoelectric point:
>tr|A0A1L3KGC4|A0A1L3KGC4_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 4 OX=1923674 PE=4 SV=1
MM1 pKa = 7.67 EE2 pKa = 4.21 SHH4 pKa = 6.92 EE5 pKa = 4.76 APGALPEE12 pKa = 4.56 GKK14 pKa = 10.09 ALPRR18 pKa = 11.84 VPADD22 pKa = 3.12 NSIYY26 pKa = 9.81 MAQLNLAASVLMLLKK41 pKa = 10.8 LCFKK45 pKa = 10.47 LAYY48 pKa = 9.29 AVYY51 pKa = 9.58 MFFATIARR59 pKa = 11.84 CLIRR63 pKa = 11.84 YY64 pKa = 8.68 VEE66 pKa = 4.14 SLLRR70 pKa = 11.84 SEE72 pKa = 5.86 LGLPDD77 pKa = 4.81 DD78 pKa = 4.49 EE79 pKa = 5.5 EE80 pKa = 5.13 IPFALITGRR89 pKa = 11.84 ILKK92 pKa = 9.65 LFHH95 pKa = 5.9 AAEE98 pKa = 4.15 RR99 pKa = 11.84 RR100 pKa = 11.84 VSAFIQMLGTDD111 pKa = 3.74 VPRR114 pKa = 11.84 LLEE117 pKa = 4.17 EE118 pKa = 5.17 LDD120 pKa = 3.62 VKK122 pKa = 11.06 DD123 pKa = 3.97 RR124 pKa = 11.84 ATSAGSRR131 pKa = 11.84 VVNEE135 pKa = 3.88 ASGLLIRR142 pKa = 11.84 AAAVVGDD149 pKa = 4.18 LRR151 pKa = 11.84 DD152 pKa = 3.62 LVAEE156 pKa = 4.47 EE157 pKa = 4.89 LPIPNPANVDD167 pKa = 3.66 PGLEE171 pKa = 4.44 GYY173 pKa = 10.14 VSPEE177 pKa = 3.45 LVLNDD182 pKa = 3.69 EE183 pKa = 4.8 GNLEE187 pKa = 3.89 EE188 pKa = 5.0 MINHH192 pKa = 6.74 EE193 pKa = 4.71 LNVAAGKK200 pKa = 9.02 MEE202 pKa = 4.43 AEE204 pKa = 4.66 CVPQEE209 pKa = 4.41 HH210 pKa = 6.23 TPQTNPVSAINEE222 pKa = 4.2 LLQRR226 pKa = 11.84 MPVTIHH232 pKa = 5.48 TYY234 pKa = 8.19 EE235 pKa = 3.85 YY236 pKa = 10.32 RR237 pKa = 11.84 DD238 pKa = 4.1 LGVKK242 pKa = 10.1 SPTYY246 pKa = 10.25 ACTMTLSNVATLKK259 pKa = 10.46 EE260 pKa = 4.05 GRR262 pKa = 11.84 LLQVTAIGGSKK273 pKa = 9.86 KK274 pKa = 9.24 VAKK277 pKa = 8.91 RR278 pKa = 11.84 TAAIRR283 pKa = 11.84 ACYY286 pKa = 9.82 ALGVKK291 pKa = 9.92 YY292 pKa = 10.31 EE293 pKa = 4.27 GMPPTPMKK301 pKa = 10.32 TSVKK305 pKa = 9.97 LIPIEE310 pKa = 4.1 TLGEE314 pKa = 3.81 ASYY317 pKa = 11.04 KK318 pKa = 10.65 KK319 pKa = 10.44 YY320 pKa = 10.94 VEE322 pKa = 3.82 PLKK325 pKa = 11.0 FFTADD330 pKa = 3.39 EE331 pKa = 4.24 NDD333 pKa = 3.71 GTGRR337 pKa = 11.84 PTMAGPVPKK346 pKa = 10.11 AAEE349 pKa = 4.17 VLSSMKK355 pKa = 10.21 EE356 pKa = 3.85 KK357 pKa = 10.26 PLKK360 pKa = 10.72 LRR362 pKa = 11.84 DD363 pKa = 3.76 AKK365 pKa = 10.66 SVSMTVKK372 pKa = 10.35 QLIEE376 pKa = 3.64 DD377 pKa = 4.44 TYY379 pKa = 10.97 RR380 pKa = 11.84 VLVRR384 pKa = 11.84 EE385 pKa = 4.08 GLKK388 pKa = 8.97 PTLAQLLEE396 pKa = 4.09 RR397 pKa = 11.84 VARR400 pKa = 11.84 NNPGPVAEE408 pKa = 4.31 VVGVVQEE415 pKa = 4.17 MEE417 pKa = 4.44 EE418 pKa = 4.2 VADD421 pKa = 3.93 THH423 pKa = 6.35 SHH425 pKa = 6.37 AGLPTVVPVPAAPMPVVYY443 pKa = 10.41 DD444 pKa = 3.38 PTGPDD449 pKa = 3.36 GEE451 pKa = 4.68 YY452 pKa = 11.0 EE453 pKa = 4.12 PMDD456 pKa = 4.13 PPGDD460 pKa = 4.09 PGEE463 pKa = 5.29 DD464 pKa = 4.18 DD465 pKa = 5.03 PPPDD469 pKa = 4.3 PPGGPDD475 pKa = 3.96 GDD477 pKa = 4.33 RR478 pKa = 11.84 LPADD482 pKa = 4.87 LDD484 pKa = 3.81 PSVVDD489 pKa = 3.17 TVVALVRR496 pKa = 11.84 RR497 pKa = 11.84 NHH499 pKa = 6.73 PSPPRR504 pKa = 11.84 NDD506 pKa = 4.22 AEE508 pKa = 4.18 AQSQRR513 pKa = 11.84 NAANAEE519 pKa = 3.64 LDD521 pKa = 3.75 AYY523 pKa = 10.25 FEE525 pKa = 4.52 TMMADD530 pKa = 3.6 GTVHH534 pKa = 6.47 ARR536 pKa = 11.84 KK537 pKa = 10.02 LMAARR542 pKa = 11.84 YY543 pKa = 8.57 IDD545 pKa = 3.7 PVVTKK550 pKa = 9.96 LEE552 pKa = 4.11 HH553 pKa = 5.64 VAPSRR558 pKa = 11.84 AEE560 pKa = 3.74 HH561 pKa = 6.13 LLVRR565 pKa = 11.84 NAPSVTAKK573 pKa = 10.35 RR574 pKa = 11.84 VLEE577 pKa = 4.0 RR578 pKa = 11.84 LGSPGKK584 pKa = 6.9 XWCRR588 pKa = 11.84 GALRR592 pKa = 11.84 GVRR595 pKa = 11.84 MLAEE599 pKa = 4.52 PDD601 pKa = 2.99 WRR603 pKa = 11.84 SVNRR607 pKa = 11.84 QLPRR611 pKa = 11.84 GYY613 pKa = 11.05 LLLFKK618 pKa = 10.26 PLYY621 pKa = 10.45 GGLASRR627 pKa = 11.84 RR628 pKa = 11.84 RR629 pKa = 11.84 LTQLVPGLSLLDD641 pKa = 3.93 FCVHH645 pKa = 7.35 DD646 pKa = 3.84 PTAYY650 pKa = 10.24 NVLDD654 pKa = 3.9 GVIQRR659 pKa = 11.84 VMLVDD664 pKa = 3.29 GHH666 pKa = 6.71 RR667 pKa = 11.84 PPQPEE672 pKa = 3.84 PGAWAALRR680 pKa = 11.84 RR681 pKa = 11.84 YY682 pKa = 9.37 ARR684 pKa = 11.84 RR685 pKa = 11.84 ITRR688 pKa = 11.84 RR689 pKa = 11.84 MPRR692 pKa = 11.84 NPKK695 pKa = 9.45 RR696 pKa = 11.84 MRR698 pKa = 11.84 RR699 pKa = 11.84 HH700 pKa = 7.28 DD701 pKa = 3.72 YY702 pKa = 8.4 PQLYY706 pKa = 9.94 GGSKK710 pKa = 9.48 RR711 pKa = 11.84 DD712 pKa = 3.49 MYY714 pKa = 11.3 AAAAVSLDD722 pKa = 3.7 EE723 pKa = 5.05 KK724 pKa = 10.76 PITSRR729 pKa = 11.84 DD730 pKa = 2.93 ARR732 pKa = 11.84 LAVFGKK738 pKa = 9.82 CEE740 pKa = 3.91 KK741 pKa = 10.58 VALAANGRR749 pKa = 11.84 PKK751 pKa = 10.92 AMRR754 pKa = 11.84 VISPRR759 pKa = 11.84 DD760 pKa = 3.14 PRR762 pKa = 11.84 FNIEE766 pKa = 4.09 LGRR769 pKa = 11.84 FLHH772 pKa = 6.67 PIEE775 pKa = 4.82 KK776 pKa = 9.61 PVYY779 pKa = 9.13 LAINAMFSNTPTTPTVQKK797 pKa = 10.33 FLNLRR802 pKa = 11.84 EE803 pKa = 3.98 RR804 pKa = 11.84 GRR806 pKa = 11.84 VLRR809 pKa = 11.84 AKK811 pKa = 9.35 WRR813 pKa = 11.84 MFRR816 pKa = 11.84 DD817 pKa = 5.21 PIAVGYY823 pKa = 8.62 DD824 pKa = 2.99 AKK826 pKa = 10.95 RR827 pKa = 11.84 FDD829 pKa = 3.51 QHH831 pKa = 5.5 TSADD835 pKa = 3.63 AMKK838 pKa = 10.16 LEE840 pKa = 3.93 HH841 pKa = 7.15 LIYY844 pKa = 10.03 TGAYY848 pKa = 8.74 ANHH851 pKa = 7.46 PEE853 pKa = 3.96 LSEE856 pKa = 4.87 LRR858 pKa = 11.84 RR859 pKa = 11.84 LLNWQLRR866 pKa = 11.84 TKK868 pKa = 9.79 ATARR872 pKa = 11.84 LKK874 pKa = 10.93 DD875 pKa = 3.49 GLIHH879 pKa = 6.98 YY880 pKa = 7.57 RR881 pKa = 11.84 TLGNRR886 pKa = 11.84 CSGDD890 pKa = 3.43 VNTSLGNIVLMCVMMRR906 pKa = 11.84 AYY908 pKa = 10.47 LDD910 pKa = 3.56 HH911 pKa = 6.27 VRR913 pKa = 11.84 LRR915 pKa = 11.84 AEE917 pKa = 4.32 LVNDD921 pKa = 3.98 GDD923 pKa = 4.18 DD924 pKa = 3.43 CVLILEE930 pKa = 4.59 RR931 pKa = 11.84 RR932 pKa = 11.84 DD933 pKa = 3.41 LALLEE938 pKa = 4.04 EE939 pKa = 4.85 LEE941 pKa = 4.43 PFFLRR946 pKa = 11.84 LGYY949 pKa = 9.44 TMIRR953 pKa = 11.84 EE954 pKa = 4.2 DD955 pKa = 3.68 PVDD958 pKa = 3.45 VFEE961 pKa = 5.66 QIEE964 pKa = 4.57 FCQCNPVFDD973 pKa = 3.96 GAEE976 pKa = 3.44 WVMVRR981 pKa = 11.84 KK982 pKa = 8.54 LTTVMSKK989 pKa = 9.3 DD990 pKa = 3.44 TITVKK995 pKa = 10.68 SLSSEE1000 pKa = 3.84 QAVRR1004 pKa = 11.84 IHH1006 pKa = 6.97 RR1007 pKa = 11.84 RR1008 pKa = 11.84 RR1009 pKa = 11.84 IMQSGMAMAGNIPVLRR1025 pKa = 11.84 SVYY1028 pKa = 10.17 KK1029 pKa = 10.37 YY1030 pKa = 10.05 IGRR1033 pKa = 11.84 GCEE1036 pKa = 3.28 AATNRR1041 pKa = 11.84 RR1042 pKa = 11.84 GRR1044 pKa = 11.84 FNPYY1048 pKa = 9.62 LDD1050 pKa = 3.95 SGLFYY1055 pKa = 9.88 ATKK1058 pKa = 10.13 GMRR1061 pKa = 11.84 YY1062 pKa = 9.91 KK1063 pKa = 10.9 DD1064 pKa = 3.58 ILPTATARR1072 pKa = 11.84 LSFWRR1077 pKa = 11.84 STGVTPSQQVSMEE1090 pKa = 4.1 KK1091 pKa = 10.33 QLDD1094 pKa = 4.09 DD1095 pKa = 5.33 LPADD1099 pKa = 4.23 PPDD1102 pKa = 3.41 MGLINHH1108 pKa = 7.07 RR1109 pKa = 11.84 FAAMMFARR1117 pKa = 11.84 HH1118 pKa = 5.43 NAKK1121 pKa = 10.05 PPNLTGSDD1129 pKa = 4.03 PLIGYY1134 pKa = 7.73 YY1135 pKa = 10.52 DD1136 pKa = 4.4 RR1137 pKa = 11.84 DD1138 pKa = 3.72 WPGPAAYY1145 pKa = 9.68 RR1146 pKa = 11.84 RR1147 pKa = 11.84 RR1148 pKa = 11.84 QAA1150 pKa = 3.48
Molecular weight: 127.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.162
IPC2_protein 8.229
IPC_protein 8.097
Toseland 8.346
ProMoST 8.639
Dawson 8.931
Bjellqvist 8.99
Wikipedia 9.019
Rodwell 9.004
Grimsley 8.624
Solomon 8.975
Lehninger 8.946
Nozaki 9.077
DTASelect 8.829
Thurlkill 8.902
EMBOSS 9.077
Sillero 9.121
Patrickios 4.635
IPC_peptide 8.975
IPC2_peptide 7.79
IPC2.peptide.svr19 7.743
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1655
505
1150
827.5
91.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.849 ± 0.026
1.269 ± 0.06
5.619 ± 0.139
5.438 ± 0.752
2.477 ± 0.049
7.13 ± 1.007
1.752 ± 0.287
3.807 ± 0.381
4.23 ± 0.137
9.728 ± 1.121
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.384 ± 0.412
3.807 ± 0.28
7.432 ± 0.658
2.598 ± 0.29
8.036 ± 0.966
5.498 ± 1.233
5.801 ± 0.978
7.795 ± 0.037
0.906 ± 0.244
3.384 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here