Wenzhou tombus-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KGC4|A0A1L3KGC4_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 4 OX=1923674 PE=4 SV=1
MM1 pKa = 7.1NTTNYY6 pKa = 10.82AGLSDD11 pKa = 5.1LLTAGSRR18 pKa = 11.84SIAYY22 pKa = 9.81SKK24 pKa = 10.82LLSAEE29 pKa = 4.0AAGQLEE35 pKa = 4.71PGAASYY41 pKa = 10.21IISALDD47 pKa = 3.56PFHH50 pKa = 7.59DD51 pKa = 3.94KK52 pKa = 10.96QYY54 pKa = 11.04DD55 pKa = 3.64ATDD58 pKa = 3.62VPSVGVQRR66 pKa = 11.84SIVRR70 pKa = 11.84VLRR73 pKa = 11.84TRR75 pKa = 11.84TRR77 pKa = 11.84ISMEE81 pKa = 3.75SATSVGYY88 pKa = 6.78TQPGRR93 pKa = 11.84LALCMYY99 pKa = 9.75PSVNSRR105 pKa = 11.84YY106 pKa = 8.69MSKK109 pKa = 10.68KK110 pKa = 10.61DD111 pKa = 3.31IVNGQAVDD119 pKa = 3.7PATAAAKK126 pKa = 10.33YY127 pKa = 10.3VGGLVWEE134 pKa = 5.21LDD136 pKa = 3.29IQGQPKK142 pKa = 9.82FFTGDD147 pKa = 3.39PSDD150 pKa = 4.13YY151 pKa = 11.12SADD154 pKa = 3.66ANNGRR159 pKa = 11.84MSIADD164 pKa = 3.6VTGGMPDD171 pKa = 3.43HH172 pKa = 6.64AHH174 pKa = 5.8MRR176 pKa = 11.84VIGCGFEE183 pKa = 3.94VHH185 pKa = 6.25NVTEE189 pKa = 4.29EE190 pKa = 3.92LSKK193 pKa = 11.34GGTVSVWQLPGIFARR208 pKa = 11.84NKK210 pKa = 9.7VVDD213 pKa = 3.82GTQVVTNAPATGIYY227 pKa = 9.43YY228 pKa = 10.56NCDD231 pKa = 3.13FLGRR235 pKa = 11.84APTTADD241 pKa = 3.11EE242 pKa = 4.54VTNIPTGQTWEE253 pKa = 4.22AGQGCLVVPRR263 pKa = 11.84LDD265 pKa = 3.81SGDD268 pKa = 3.85FLLAGCNTPNAEE280 pKa = 4.04ALIEE284 pKa = 4.23GVWDD288 pKa = 3.92GNGTYY293 pKa = 10.2AYY295 pKa = 10.02FPTGSNTSVRR305 pKa = 11.84TFSTPFSMGGALFEE319 pKa = 4.37GLPPEE324 pKa = 4.25SVLDD328 pKa = 4.3VEE330 pKa = 5.3CKK332 pKa = 9.84WIVAEE337 pKa = 4.44VPHH340 pKa = 7.47VSDD343 pKa = 4.71IGDD346 pKa = 3.53LVIARR351 pKa = 11.84NTPPGMFPHH360 pKa = 5.43TTKK363 pKa = 11.13LIACLNRR370 pKa = 11.84TLRR373 pKa = 11.84DD374 pKa = 3.85GYY376 pKa = 9.36PAGWNEE382 pKa = 3.89SGKK385 pKa = 9.96YY386 pKa = 7.15WSSVLRR392 pKa = 11.84AISSVLPMIGNAVPIPGAKK411 pKa = 10.12LLTSAASAALQQKK424 pKa = 9.35ARR426 pKa = 11.84NRR428 pKa = 11.84DD429 pKa = 3.29EE430 pKa = 5.36TIDD433 pKa = 3.74LMKK436 pKa = 10.25DD437 pKa = 3.43LKK439 pKa = 10.55SARR442 pKa = 11.84PGTKK446 pKa = 9.29EE447 pKa = 3.48YY448 pKa = 11.47NEE450 pKa = 5.05LYY452 pKa = 9.59RR453 pKa = 11.84QAMANAGPIQRR464 pKa = 11.84RR465 pKa = 11.84QLRR468 pKa = 11.84RR469 pKa = 11.84LRR471 pKa = 11.84RR472 pKa = 11.84ADD474 pKa = 3.12EE475 pKa = 4.07RR476 pKa = 11.84TVKK479 pKa = 10.23GFTMVSPSTGSKK491 pKa = 9.08TKK493 pKa = 10.85LPTSSIAVVKK503 pKa = 10.58RR504 pKa = 11.84RR505 pKa = 3.48

Molecular weight:
54.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KGC4|A0A1L3KGC4_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 4 OX=1923674 PE=4 SV=1
MM1 pKa = 7.67EE2 pKa = 4.21SHH4 pKa = 6.92EE5 pKa = 4.76APGALPEE12 pKa = 4.56GKK14 pKa = 10.09ALPRR18 pKa = 11.84VPADD22 pKa = 3.12NSIYY26 pKa = 9.81MAQLNLAASVLMLLKK41 pKa = 10.8LCFKK45 pKa = 10.47LAYY48 pKa = 9.29AVYY51 pKa = 9.58MFFATIARR59 pKa = 11.84CLIRR63 pKa = 11.84YY64 pKa = 8.68VEE66 pKa = 4.14SLLRR70 pKa = 11.84SEE72 pKa = 5.86LGLPDD77 pKa = 4.81DD78 pKa = 4.49EE79 pKa = 5.5EE80 pKa = 5.13IPFALITGRR89 pKa = 11.84ILKK92 pKa = 9.65LFHH95 pKa = 5.9AAEE98 pKa = 4.15RR99 pKa = 11.84RR100 pKa = 11.84VSAFIQMLGTDD111 pKa = 3.74VPRR114 pKa = 11.84LLEE117 pKa = 4.17EE118 pKa = 5.17LDD120 pKa = 3.62VKK122 pKa = 11.06DD123 pKa = 3.97RR124 pKa = 11.84ATSAGSRR131 pKa = 11.84VVNEE135 pKa = 3.88ASGLLIRR142 pKa = 11.84AAAVVGDD149 pKa = 4.18LRR151 pKa = 11.84DD152 pKa = 3.62LVAEE156 pKa = 4.47EE157 pKa = 4.89LPIPNPANVDD167 pKa = 3.66PGLEE171 pKa = 4.44GYY173 pKa = 10.14VSPEE177 pKa = 3.45LVLNDD182 pKa = 3.69EE183 pKa = 4.8GNLEE187 pKa = 3.89EE188 pKa = 5.0MINHH192 pKa = 6.74EE193 pKa = 4.71LNVAAGKK200 pKa = 9.02MEE202 pKa = 4.43AEE204 pKa = 4.66CVPQEE209 pKa = 4.41HH210 pKa = 6.23TPQTNPVSAINEE222 pKa = 4.2LLQRR226 pKa = 11.84MPVTIHH232 pKa = 5.48TYY234 pKa = 8.19EE235 pKa = 3.85YY236 pKa = 10.32RR237 pKa = 11.84DD238 pKa = 4.1LGVKK242 pKa = 10.1SPTYY246 pKa = 10.25ACTMTLSNVATLKK259 pKa = 10.46EE260 pKa = 4.05GRR262 pKa = 11.84LLQVTAIGGSKK273 pKa = 9.86KK274 pKa = 9.24VAKK277 pKa = 8.91RR278 pKa = 11.84TAAIRR283 pKa = 11.84ACYY286 pKa = 9.82ALGVKK291 pKa = 9.92YY292 pKa = 10.31EE293 pKa = 4.27GMPPTPMKK301 pKa = 10.32TSVKK305 pKa = 9.97LIPIEE310 pKa = 4.1TLGEE314 pKa = 3.81ASYY317 pKa = 11.04KK318 pKa = 10.65KK319 pKa = 10.44YY320 pKa = 10.94VEE322 pKa = 3.82PLKK325 pKa = 11.0FFTADD330 pKa = 3.39EE331 pKa = 4.24NDD333 pKa = 3.71GTGRR337 pKa = 11.84PTMAGPVPKK346 pKa = 10.11AAEE349 pKa = 4.17VLSSMKK355 pKa = 10.21EE356 pKa = 3.85KK357 pKa = 10.26PLKK360 pKa = 10.72LRR362 pKa = 11.84DD363 pKa = 3.76AKK365 pKa = 10.66SVSMTVKK372 pKa = 10.35QLIEE376 pKa = 3.64DD377 pKa = 4.44TYY379 pKa = 10.97RR380 pKa = 11.84VLVRR384 pKa = 11.84EE385 pKa = 4.08GLKK388 pKa = 8.97PTLAQLLEE396 pKa = 4.09RR397 pKa = 11.84VARR400 pKa = 11.84NNPGPVAEE408 pKa = 4.31VVGVVQEE415 pKa = 4.17MEE417 pKa = 4.44EE418 pKa = 4.2VADD421 pKa = 3.93THH423 pKa = 6.35SHH425 pKa = 6.37AGLPTVVPVPAAPMPVVYY443 pKa = 10.41DD444 pKa = 3.38PTGPDD449 pKa = 3.36GEE451 pKa = 4.68YY452 pKa = 11.0EE453 pKa = 4.12PMDD456 pKa = 4.13PPGDD460 pKa = 4.09PGEE463 pKa = 5.29DD464 pKa = 4.18DD465 pKa = 5.03PPPDD469 pKa = 4.3PPGGPDD475 pKa = 3.96GDD477 pKa = 4.33RR478 pKa = 11.84LPADD482 pKa = 4.87LDD484 pKa = 3.81PSVVDD489 pKa = 3.17TVVALVRR496 pKa = 11.84RR497 pKa = 11.84NHH499 pKa = 6.73PSPPRR504 pKa = 11.84NDD506 pKa = 4.22AEE508 pKa = 4.18AQSQRR513 pKa = 11.84NAANAEE519 pKa = 3.64LDD521 pKa = 3.75AYY523 pKa = 10.25FEE525 pKa = 4.52TMMADD530 pKa = 3.6GTVHH534 pKa = 6.47ARR536 pKa = 11.84KK537 pKa = 10.02LMAARR542 pKa = 11.84YY543 pKa = 8.57IDD545 pKa = 3.7PVVTKK550 pKa = 9.96LEE552 pKa = 4.11HH553 pKa = 5.64VAPSRR558 pKa = 11.84AEE560 pKa = 3.74HH561 pKa = 6.13LLVRR565 pKa = 11.84NAPSVTAKK573 pKa = 10.35RR574 pKa = 11.84VLEE577 pKa = 4.0RR578 pKa = 11.84LGSPGKK584 pKa = 6.9XWCRR588 pKa = 11.84GALRR592 pKa = 11.84GVRR595 pKa = 11.84MLAEE599 pKa = 4.52PDD601 pKa = 2.99WRR603 pKa = 11.84SVNRR607 pKa = 11.84QLPRR611 pKa = 11.84GYY613 pKa = 11.05LLLFKK618 pKa = 10.26PLYY621 pKa = 10.45GGLASRR627 pKa = 11.84RR628 pKa = 11.84RR629 pKa = 11.84LTQLVPGLSLLDD641 pKa = 3.93FCVHH645 pKa = 7.35DD646 pKa = 3.84PTAYY650 pKa = 10.24NVLDD654 pKa = 3.9GVIQRR659 pKa = 11.84VMLVDD664 pKa = 3.29GHH666 pKa = 6.71RR667 pKa = 11.84PPQPEE672 pKa = 3.84PGAWAALRR680 pKa = 11.84RR681 pKa = 11.84YY682 pKa = 9.37ARR684 pKa = 11.84RR685 pKa = 11.84ITRR688 pKa = 11.84RR689 pKa = 11.84MPRR692 pKa = 11.84NPKK695 pKa = 9.45RR696 pKa = 11.84MRR698 pKa = 11.84RR699 pKa = 11.84HH700 pKa = 7.28DD701 pKa = 3.72YY702 pKa = 8.4PQLYY706 pKa = 9.94GGSKK710 pKa = 9.48RR711 pKa = 11.84DD712 pKa = 3.49MYY714 pKa = 11.3AAAAVSLDD722 pKa = 3.7EE723 pKa = 5.05KK724 pKa = 10.76PITSRR729 pKa = 11.84DD730 pKa = 2.93ARR732 pKa = 11.84LAVFGKK738 pKa = 9.82CEE740 pKa = 3.91KK741 pKa = 10.58VALAANGRR749 pKa = 11.84PKK751 pKa = 10.92AMRR754 pKa = 11.84VISPRR759 pKa = 11.84DD760 pKa = 3.14PRR762 pKa = 11.84FNIEE766 pKa = 4.09LGRR769 pKa = 11.84FLHH772 pKa = 6.67PIEE775 pKa = 4.82KK776 pKa = 9.61PVYY779 pKa = 9.13LAINAMFSNTPTTPTVQKK797 pKa = 10.33FLNLRR802 pKa = 11.84EE803 pKa = 3.98RR804 pKa = 11.84GRR806 pKa = 11.84VLRR809 pKa = 11.84AKK811 pKa = 9.35WRR813 pKa = 11.84MFRR816 pKa = 11.84DD817 pKa = 5.21PIAVGYY823 pKa = 8.62DD824 pKa = 2.99AKK826 pKa = 10.95RR827 pKa = 11.84FDD829 pKa = 3.51QHH831 pKa = 5.5TSADD835 pKa = 3.63AMKK838 pKa = 10.16LEE840 pKa = 3.93HH841 pKa = 7.15LIYY844 pKa = 10.03TGAYY848 pKa = 8.74ANHH851 pKa = 7.46PEE853 pKa = 3.96LSEE856 pKa = 4.87LRR858 pKa = 11.84RR859 pKa = 11.84LLNWQLRR866 pKa = 11.84TKK868 pKa = 9.79ATARR872 pKa = 11.84LKK874 pKa = 10.93DD875 pKa = 3.49GLIHH879 pKa = 6.98YY880 pKa = 7.57RR881 pKa = 11.84TLGNRR886 pKa = 11.84CSGDD890 pKa = 3.43VNTSLGNIVLMCVMMRR906 pKa = 11.84AYY908 pKa = 10.47LDD910 pKa = 3.56HH911 pKa = 6.27VRR913 pKa = 11.84LRR915 pKa = 11.84AEE917 pKa = 4.32LVNDD921 pKa = 3.98GDD923 pKa = 4.18DD924 pKa = 3.43CVLILEE930 pKa = 4.59RR931 pKa = 11.84RR932 pKa = 11.84DD933 pKa = 3.41LALLEE938 pKa = 4.04EE939 pKa = 4.85LEE941 pKa = 4.43PFFLRR946 pKa = 11.84LGYY949 pKa = 9.44TMIRR953 pKa = 11.84EE954 pKa = 4.2DD955 pKa = 3.68PVDD958 pKa = 3.45VFEE961 pKa = 5.66QIEE964 pKa = 4.57FCQCNPVFDD973 pKa = 3.96GAEE976 pKa = 3.44WVMVRR981 pKa = 11.84KK982 pKa = 8.54LTTVMSKK989 pKa = 9.3DD990 pKa = 3.44TITVKK995 pKa = 10.68SLSSEE1000 pKa = 3.84QAVRR1004 pKa = 11.84IHH1006 pKa = 6.97RR1007 pKa = 11.84RR1008 pKa = 11.84RR1009 pKa = 11.84IMQSGMAMAGNIPVLRR1025 pKa = 11.84SVYY1028 pKa = 10.17KK1029 pKa = 10.37YY1030 pKa = 10.05IGRR1033 pKa = 11.84GCEE1036 pKa = 3.28AATNRR1041 pKa = 11.84RR1042 pKa = 11.84GRR1044 pKa = 11.84FNPYY1048 pKa = 9.62LDD1050 pKa = 3.95SGLFYY1055 pKa = 9.88ATKK1058 pKa = 10.13GMRR1061 pKa = 11.84YY1062 pKa = 9.91KK1063 pKa = 10.9DD1064 pKa = 3.58ILPTATARR1072 pKa = 11.84LSFWRR1077 pKa = 11.84STGVTPSQQVSMEE1090 pKa = 4.1KK1091 pKa = 10.33QLDD1094 pKa = 4.09DD1095 pKa = 5.33LPADD1099 pKa = 4.23PPDD1102 pKa = 3.41MGLINHH1108 pKa = 7.07RR1109 pKa = 11.84FAAMMFARR1117 pKa = 11.84HH1118 pKa = 5.43NAKK1121 pKa = 10.05PPNLTGSDD1129 pKa = 4.03PLIGYY1134 pKa = 7.73YY1135 pKa = 10.52DD1136 pKa = 4.4RR1137 pKa = 11.84DD1138 pKa = 3.72WPGPAAYY1145 pKa = 9.68RR1146 pKa = 11.84RR1147 pKa = 11.84RR1148 pKa = 11.84QAA1150 pKa = 3.48

Molecular weight:
127.99 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1655

505

1150

827.5

91.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.849 ± 0.026

1.269 ± 0.06

5.619 ± 0.139

5.438 ± 0.752

2.477 ± 0.049

7.13 ± 1.007

1.752 ± 0.287

3.807 ± 0.381

4.23 ± 0.137

9.728 ± 1.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.384 ± 0.412

3.807 ± 0.28

7.432 ± 0.658

2.598 ± 0.29

8.036 ± 0.966

5.498 ± 1.233

5.801 ± 0.978

7.795 ± 0.037

0.906 ± 0.244

3.384 ± 0.009

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski