Bordetella phage vB_BbrM_PHB04

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phabquatrovirus; Bordetella virus PHB04

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291LAS4|A0A291LAS4_9CAUD VRR-NUC domain-containing protein OS=Bordetella phage vB_BbrM_PHB04 OX=2029657 PE=4 SV=1
MM1 pKa = 7.43QRR3 pKa = 11.84DD4 pKa = 3.36DD5 pKa = 3.85WLDD8 pKa = 3.7DD9 pKa = 3.83KK10 pKa = 11.43KK11 pKa = 11.07DD12 pKa = 3.64PQLEE16 pKa = 4.1CDD18 pKa = 3.44EE19 pKa = 5.16AMTPLEE25 pKa = 4.43AACMTLCVLILVVGFGTLAYY45 pKa = 8.89WNWFAA50 pKa = 5.81

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291LAP9|A0A291LAP9_9CAUD Uncharacterized protein OS=Bordetella phage vB_BbrM_PHB04 OX=2029657 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84IHH4 pKa = 7.43ISTAPARR11 pKa = 11.84PRR13 pKa = 11.84PQVGDD18 pKa = 3.36RR19 pKa = 11.84KK20 pKa = 9.99FIRR23 pKa = 11.84GVEE26 pKa = 4.13HH27 pKa = 6.33VRR29 pKa = 11.84VLEE32 pKa = 4.25TVKK35 pKa = 10.72SGPHH39 pKa = 5.23RR40 pKa = 11.84GAYY43 pKa = 8.98IRR45 pKa = 11.84TNSGPCYY52 pKa = 10.08EE53 pKa = 5.27WIPLDD58 pKa = 3.66AAPRR62 pKa = 11.84YY63 pKa = 9.52LRR65 pKa = 11.84DD66 pKa = 3.37MPGRR70 pKa = 11.84LIGKK74 pKa = 9.16RR75 pKa = 11.84PEE77 pKa = 4.29DD78 pKa = 3.57ASRR81 pKa = 11.84DD82 pKa = 3.63

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

124

0

124

28484

37

2097

229.7

25.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.905 ± 0.277

0.888 ± 0.107

6.565 ± 0.244

6.14 ± 0.233

3.293 ± 0.139

8.313 ± 0.262

2.036 ± 0.141

4.476 ± 0.135

4.631 ± 0.207

8.082 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.11

3.286 ± 0.123

5.105 ± 0.148

4.027 ± 0.175

7.222 ± 0.213

4.982 ± 0.162

5.536 ± 0.215

6.695 ± 0.191

1.471 ± 0.084

2.637 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski