Helicobacter sp. MIT 99-10781

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; unclassified Helicobacter

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1914 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8HN75|A0A3D8HN75_9HELI Chemotaxis protein CheW OS=Helicobacter sp. MIT 99-10781 OX=1332285 GN=CQA37_07930 PE=4 SV=1
MM1 pKa = 6.75TTLLSVIYY9 pKa = 9.44TKK11 pKa = 10.99ASDD14 pKa = 3.78YY15 pKa = 10.75KK16 pKa = 10.11ICNSCGCFNFYY27 pKa = 11.04DD28 pKa = 3.71RR29 pKa = 11.84DD30 pKa = 3.79FCHH33 pKa = 6.76EE34 pKa = 4.27CGEE37 pKa = 4.36TSFDD41 pKa = 3.79DD42 pKa = 3.61SLEE45 pKa = 4.03RR46 pKa = 11.84VQDD49 pKa = 3.39EE50 pKa = 4.23TRR52 pKa = 11.84RR53 pKa = 11.84EE54 pKa = 3.21IDD56 pKa = 4.29FYY58 pKa = 11.34FDD60 pKa = 2.94EE61 pKa = 5.73CGYY64 pKa = 10.99DD65 pKa = 3.12WEE67 pKa = 4.62EE68 pKa = 4.1VMNLEE73 pKa = 5.13IGII76 pKa = 4.3

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8HQ35|A0A3D8HQ35_9HELI Uncharacterized protein OS=Helicobacter sp. MIT 99-10781 OX=1332285 GN=CQA37_06775 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.32RR14 pKa = 11.84THH16 pKa = 6.11GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.44TKK25 pKa = 10.15NGRR28 pKa = 11.84RR29 pKa = 11.84VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.49GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1914

0

1914

601259

25

2887

314.1

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.885 ± 0.042

1.267 ± 0.023

4.795 ± 0.034

6.705 ± 0.063

5.407 ± 0.049

6.183 ± 0.064

1.745 ± 0.021

7.461 ± 0.045

7.653 ± 0.057

10.633 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.022

5.358 ± 0.057

3.203 ± 0.031

4.089 ± 0.045

3.884 ± 0.035

7.427 ± 0.04

4.531 ± 0.055

5.518 ± 0.057

0.72 ± 0.016

3.449 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski