Aeromonas phage LAh3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Ahphunavirus; unclassified Ahphunavirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A513ZZ75|A0A513ZZ75_9CAUD Putative tail tubular protein B OS=Aeromonas phage LAh3 OX=2591027 GN=LAh3_5 PE=4 SV=1
MM1 pKa = 7.77LFRR4 pKa = 11.84NQALKK9 pKa = 11.0YY10 pKa = 8.73MNAAADD16 pKa = 4.32DD17 pKa = 4.43GAQGGGGVPGVVPGAEE33 pKa = 3.97PAAPAPIGPGAGKK46 pKa = 9.97AAEE49 pKa = 3.95EE50 pKa = 4.13AAAKK54 pKa = 9.93AAAEE58 pKa = 4.2SKK60 pKa = 10.53AAEE63 pKa = 3.92EE64 pKa = 3.72AAAAEE69 pKa = 4.24EE70 pKa = 4.18AAKK73 pKa = 10.32KK74 pKa = 10.18AAEE77 pKa = 4.01QNKK80 pKa = 9.34KK81 pKa = 10.21PEE83 pKa = 4.22NTTPPAEE90 pKa = 4.25GSIEE94 pKa = 4.1GYY96 pKa = 9.8IEE98 pKa = 4.99HH99 pKa = 6.69FQQSKK104 pKa = 9.42PALSLALTFLKK115 pKa = 10.88DD116 pKa = 3.71AGITSADD123 pKa = 3.62PAFQLAEE130 pKa = 4.11SSNDD134 pKa = 3.26FTLLEE139 pKa = 4.28AVLAQKK145 pKa = 10.31GLPGTDD151 pKa = 2.78AMVSILKK158 pKa = 10.67AEE160 pKa = 4.25AAANAATLEE169 pKa = 4.29AEE171 pKa = 4.47QKK173 pKa = 9.96ATDD176 pKa = 3.86EE177 pKa = 4.18LVAGILGDD185 pKa = 3.87DD186 pKa = 4.39AEE188 pKa = 6.23AILDD192 pKa = 3.82WAVANADD199 pKa = 3.91DD200 pKa = 4.77AEE202 pKa = 5.04KK203 pKa = 10.59EE204 pKa = 4.27ALDD207 pKa = 3.86SMLNAGGVYY216 pKa = 10.21ARR218 pKa = 11.84AAAIMLQQAYY228 pKa = 9.32NGSSEE233 pKa = 4.38TVPAKK238 pKa = 10.27AVPPTKK244 pKa = 9.84TAPTQPAGVVTASQFAAEE262 pKa = 4.24SQKK265 pKa = 11.15LYY267 pKa = 11.2AKK269 pKa = 10.24YY270 pKa = 10.33GQDD273 pKa = 3.2FKK275 pKa = 11.04QTQEE279 pKa = 3.97YY280 pKa = 10.49AKK282 pKa = 10.46LSSARR287 pKa = 11.84AAARR291 pKa = 11.84AKK293 pKa = 10.73GLL295 pKa = 3.6

Molecular weight: 29.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A513ZZC0|A0A513ZZC0_9CAUD Putative DNA exonuclease OS=Aeromonas phage LAh3 OX=2591027 GN=LAh3_41 PE=4 SV=1
MM1 pKa = 7.71PKK3 pKa = 9.96LRR5 pKa = 11.84KK6 pKa = 9.09PDD8 pKa = 3.26TSDD11 pKa = 2.77IFGGRR16 pKa = 11.84LKK18 pKa = 10.72YY19 pKa = 10.74VEE21 pKa = 4.56LACMTKK27 pKa = 10.47GQAKK31 pKa = 9.77RR32 pKa = 11.84KK33 pKa = 9.38LRR35 pKa = 11.84AAGCGPLRR43 pKa = 11.84EE44 pKa = 4.83DD45 pKa = 3.31RR46 pKa = 11.84TSYY49 pKa = 11.0SPEE52 pKa = 3.71LVQKK56 pKa = 10.39DD57 pKa = 3.53GRR59 pKa = 11.84GRR61 pKa = 11.84PFGRR65 pKa = 11.84AYY67 pKa = 10.9RR68 pKa = 11.84MASGWVVHH76 pKa = 6.11LAKK79 pKa = 10.62KK80 pKa = 10.15

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

13354

21

1252

267.1

29.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.315 ± 0.641

1.281 ± 0.172

5.848 ± 0.198

5.856 ± 0.249

3.257 ± 0.208

7.893 ± 0.281

2.112 ± 0.184

4.194 ± 0.225

5.729 ± 0.299

8.814 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.108 ± 0.172

3.894 ± 0.259

3.999 ± 0.243

4.68 ± 0.336

5.848 ± 0.266

5.467 ± 0.195

5.818 ± 0.278

6.283 ± 0.227

1.415 ± 0.144

3.19 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski