Capybara microvirus Cap1_SP_128
Average proteome isoelectric point is 5.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W499|A0A4P8W499_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_128 OX=2585381 PE=4 SV=1
MM1 pKa = 7.61 IYY3 pKa = 10.24 FYY5 pKa = 11.55 VVFYY9 pKa = 11.18 KK10 pKa = 10.57 KK11 pKa = 10.9 GNDD14 pKa = 3.79 YY15 pKa = 10.08 QWSYY19 pKa = 11.65 PFMHH23 pKa = 7.07 PSIDD27 pKa = 3.19 GVTNYY32 pKa = 9.88 IIEE35 pKa = 4.11 QLGVDD40 pKa = 3.48 VEE42 pKa = 4.44 IIKK45 pKa = 10.01 VIRR48 pKa = 11.84 LSCCDD53 pKa = 3.45 MNNQVMIPYY62 pKa = 9.82 RR63 pKa = 11.84 EE64 pKa = 4.06 EE65 pKa = 4.02 IYY67 pKa = 11.01 VDD69 pKa = 3.38 EE70 pKa = 5.34 KK71 pKa = 11.29 LDD73 pKa = 3.9 QEE75 pKa = 4.07 FSKK78 pKa = 10.59 IKK80 pKa = 10.21 KK81 pKa = 9.46 EE82 pKa = 4.65 KK83 pKa = 9.3 EE84 pKa = 3.5 LQYY87 pKa = 11.05 EE88 pKa = 4.03 KK89 pKa = 11.29 NEE91 pKa = 4.02 NVEE94 pKa = 4.17 SEE96 pKa = 4.38 EE97 pKa = 4.01
Molecular weight: 11.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.413
IPC2_protein 4.635
IPC_protein 4.495
Toseland 4.355
ProMoST 4.52
Dawson 4.418
Bjellqvist 4.622
Wikipedia 4.266
Rodwell 4.329
Grimsley 4.266
Solomon 4.418
Lehninger 4.368
Nozaki 4.533
DTASelect 4.635
Thurlkill 4.342
EMBOSS 4.291
Sillero 4.596
Patrickios 2.931
IPC_peptide 4.431
IPC2_peptide 4.596
IPC2.peptide.svr19 4.54
Protein with the highest isoelectric point:
>tr|A0A4P8W7W7|A0A4P8W7W7_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_128 OX=2585381 PE=3 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.87 CLNKK6 pKa = 10.21 CHH8 pKa = 6.77 VLISPNRR15 pKa = 11.84 VIKK18 pKa = 10.66 KK19 pKa = 10.38 DD20 pKa = 3.28 GTLGNRR26 pKa = 11.84 VDD28 pKa = 4.55 FFHH31 pKa = 7.11 GLLSPVEE38 pKa = 4.14 QSLLPAEE45 pKa = 4.36 VRR47 pKa = 11.84 YY48 pKa = 10.61 EE49 pKa = 3.83 EE50 pKa = 4.65 VPCGKK55 pKa = 9.95 CVNCKK60 pKa = 9.67 RR61 pKa = 11.84 SKK63 pKa = 10.7 SYY65 pKa = 11.29 AQFLRR70 pKa = 11.84 LKK72 pKa = 9.42 MEE74 pKa = 3.82 QYY76 pKa = 10.66 SYY78 pKa = 11.09 KK79 pKa = 10.8 SSDD82 pKa = 2.92 ICFMTFTYY90 pKa = 10.95 DD91 pKa = 3.53 NYY93 pKa = 10.86 HH94 pKa = 6.12 LPKK97 pKa = 10.56 DD98 pKa = 3.49 RR99 pKa = 11.84 LHH101 pKa = 6.84 NYY103 pKa = 10.06 RR104 pKa = 11.84 DD105 pKa = 3.43 FQLFMKK111 pKa = 10.21 RR112 pKa = 11.84 FRR114 pKa = 11.84 KK115 pKa = 9.6 HH116 pKa = 6.12 FPADD120 pKa = 4.14 DD121 pKa = 3.01 IRR123 pKa = 11.84 YY124 pKa = 8.25 YY125 pKa = 10.95 CVGEE129 pKa = 3.68 YY130 pKa = 10.43 GEE132 pKa = 4.9 RR133 pKa = 11.84 YY134 pKa = 9.2 GRR136 pKa = 11.84 PHH138 pKa = 5.32 YY139 pKa = 10.34 HH140 pKa = 6.35 VILFGVDD147 pKa = 3.42 LFSIADD153 pKa = 3.56 YY154 pKa = 11.28 HH155 pKa = 7.38 DD156 pKa = 3.78 VNEE159 pKa = 4.72 NGNILYY165 pKa = 9.86 YY166 pKa = 11.22 SNVIDD171 pKa = 4.35 KK172 pKa = 10.3 LWNNGLVNFSEE183 pKa = 4.59 VNDD186 pKa = 3.64 NVFGYY191 pKa = 10.17 VSQYY195 pKa = 9.8 GVKK198 pKa = 10.37 SLTSEE203 pKa = 4.27 DD204 pKa = 3.67 KK205 pKa = 10.81 PLVRR209 pKa = 11.84 SSRR212 pKa = 11.84 RR213 pKa = 11.84 GGLGYY218 pKa = 10.71 RR219 pKa = 11.84 FLDD222 pKa = 3.95 DD223 pKa = 5.31 NKK225 pKa = 11.28 DD226 pKa = 3.59 QIIKK230 pKa = 10.41 NGYY233 pKa = 8.67 ILLDD237 pKa = 3.69 GKK239 pKa = 10.82 KK240 pKa = 9.48 YY241 pKa = 10.95 NCDD244 pKa = 2.8 KK245 pKa = 10.71 YY246 pKa = 10.85 VRR248 pKa = 11.84 SFLGVSDD255 pKa = 4.29 EE256 pKa = 3.88 WTADD260 pKa = 3.39 YY261 pKa = 10.63 RR262 pKa = 11.84 SKK264 pKa = 11.26 LDD266 pKa = 3.48 YY267 pKa = 10.35 QHH269 pKa = 6.65 SIKK272 pKa = 10.96 VKK274 pKa = 7.48 TTSLSDD280 pKa = 4.01 DD281 pKa = 4.12 YY282 pKa = 11.5 INNILAQKK290 pKa = 9.9 YY291 pKa = 7.23 EE292 pKa = 3.82 EE293 pKa = 4.13 RR294 pKa = 11.84 QRR296 pKa = 11.84 AKK298 pKa = 10.94 ALAKK302 pKa = 10.49 KK303 pKa = 10.18 LL304 pKa = 3.7
Molecular weight: 35.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.007
IPC2_protein 8.126
IPC_protein 7.995
Toseland 7.966
ProMoST 8.478
Dawson 8.726
Bjellqvist 8.873
Wikipedia 8.756
Rodwell 8.77
Grimsley 7.98
Solomon 8.814
Lehninger 8.785
Nozaki 8.931
DTASelect 8.682
Thurlkill 8.741
EMBOSS 8.887
Sillero 8.99
Patrickios 3.948
IPC_peptide 8.799
IPC2_peptide 7.732
IPC2.peptide.svr19 7.883
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1304
97
539
260.8
29.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.525 ± 1.179
1.304 ± 0.44
7.439 ± 0.636
4.908 ± 0.926
4.601 ± 0.174
6.442 ± 0.465
2.147 ± 0.452
6.135 ± 0.743
5.598 ± 1.316
7.592 ± 0.824
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.352
6.212 ± 0.737
4.601 ± 1.122
4.371 ± 0.403
4.371 ± 0.647
9.893 ± 1.296
4.525 ± 1.115
6.288 ± 0.593
1.227 ± 0.319
5.675 ± 0.969
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here