Mycobacterium phage Flathead

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WIR4|A0A5Q2WIR4_9CAUD Uncharacterized protein OS=Mycobacterium phage Flathead OX=2653756 GN=84 PE=4 SV=1
MM1 pKa = 7.86PLLIAAGIIGPGLAAPEE18 pKa = 4.19AKK20 pKa = 10.36ADD22 pKa = 3.49ITSDD26 pKa = 3.54AFVMALDD33 pKa = 4.35SEE35 pKa = 5.11GITYY39 pKa = 10.39SSKK42 pKa = 10.3PAVINAGKK50 pKa = 10.01AVCDD54 pKa = 3.93VLDD57 pKa = 3.65TGYY60 pKa = 9.51TMYY63 pKa = 10.19EE64 pKa = 3.7ASVFVYY70 pKa = 10.84NNSNLNLYY78 pKa = 10.53DD79 pKa = 2.88SGYY82 pKa = 10.68FVGAATASFCPEE94 pKa = 4.1HH95 pKa = 7.29LSGTGWVV102 pKa = 3.57

Molecular weight: 10.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WL44|A0A5Q2WL44_9CAUD Uncharacterized protein OS=Mycobacterium phage Flathead OX=2653756 GN=51 PE=4 SV=1
MM1 pKa = 7.51WRR3 pKa = 11.84RR4 pKa = 11.84ASEE7 pKa = 4.05VSAYY11 pKa = 8.87TVASLITHH19 pKa = 6.6EE20 pKa = 4.53SWLDD24 pKa = 3.31TRR26 pKa = 11.84SPLGQITVNGRR37 pKa = 11.84TPAEE41 pKa = 4.11TEE43 pKa = 3.82NLIDD47 pKa = 3.91RR48 pKa = 11.84VRR50 pKa = 11.84EE51 pKa = 3.85ALAGRR56 pKa = 11.84GYY58 pKa = 9.7SVRR61 pKa = 11.84RR62 pKa = 11.84SAQRR66 pKa = 11.84PILIIKK72 pKa = 9.26KK73 pKa = 10.0

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

18163

25

1176

168.2

18.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.632 ± 0.463

1.305 ± 0.179

6.645 ± 0.218

5.687 ± 0.256

2.94 ± 0.181

8.859 ± 0.655

2.235 ± 0.197

4.427 ± 0.155

3.21 ± 0.154

7.301 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.112

3.38 ± 0.132

6.221 ± 0.2

3.612 ± 0.198

6.711 ± 0.363

6.139 ± 0.259

6.508 ± 0.232

7.102 ± 0.234

2.29 ± 0.141

2.511 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski