Brevibacillus sp. SYP-B805

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus; unclassified Brevibacillus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3961 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1UCW3|A0A6M1UCW3_9BACL Glutamine synthetase OS=Brevibacillus sp. SYP-B805 OX=1578199 GN=glnA PE=3 SV=1
MM1 pKa = 7.59EE2 pKa = 5.17SLAKK6 pKa = 10.15RR7 pKa = 11.84ISYY10 pKa = 9.92LRR12 pKa = 11.84GWTDD16 pKa = 3.14GLEE19 pKa = 4.21LEE21 pKa = 4.45EE22 pKa = 4.42SSRR25 pKa = 11.84EE26 pKa = 3.87GRR28 pKa = 11.84ILSEE32 pKa = 4.33IIQILDD38 pKa = 3.42EE39 pKa = 4.19MSGEE43 pKa = 3.94LRR45 pKa = 11.84ALNARR50 pKa = 11.84VEE52 pKa = 4.35EE53 pKa = 4.36TEE55 pKa = 4.49SYY57 pKa = 11.01VEE59 pKa = 5.65AVDD62 pKa = 4.67EE63 pKa = 4.83DD64 pKa = 4.24LADD67 pKa = 4.54LEE69 pKa = 4.33WFLYY73 pKa = 10.74DD74 pKa = 5.3EE75 pKa = 5.12EE76 pKa = 6.72DD77 pKa = 3.91DD78 pKa = 5.07LYY80 pKa = 11.13EE81 pKa = 4.54TVDD84 pKa = 3.93DD85 pKa = 5.07DD86 pKa = 4.17EE87 pKa = 5.5AAAVYY92 pKa = 10.63DD93 pKa = 4.64LDD95 pKa = 4.58DD96 pKa = 5.99SEE98 pKa = 5.36DD99 pKa = 3.08AWVYY103 pKa = 10.92DD104 pKa = 3.45RR105 pKa = 11.84WRR107 pKa = 11.84DD108 pKa = 3.31RR109 pKa = 11.84GEE111 pKa = 4.19AYY113 pKa = 7.45EE114 pKa = 4.04TAYY117 pKa = 9.98EE118 pKa = 4.78FPCPSCQEE126 pKa = 3.85IIFLHH131 pKa = 6.77EE132 pKa = 4.17STDD135 pKa = 3.64DD136 pKa = 3.52EE137 pKa = 5.5GYY139 pKa = 8.8HH140 pKa = 6.51HH141 pKa = 6.8YY142 pKa = 10.75VIEE145 pKa = 4.09PCRR148 pKa = 11.84DD149 pKa = 2.87QRR151 pKa = 11.84EE152 pKa = 4.53PINPTT157 pKa = 3.11

Molecular weight: 18.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1U3I6|A0A6M1U3I6_9BACL Dihydrodipicolinate synthase family protein OS=Brevibacillus sp. SYP-B805 OX=1578199 GN=G3578_00080 PE=4 SV=1
MM1 pKa = 7.53TGRR4 pKa = 11.84GLLGVILTSGLIAGIGYY21 pKa = 8.3MLLPQRR27 pKa = 11.84KK28 pKa = 8.17NRR30 pKa = 11.84FTRR33 pKa = 11.84RR34 pKa = 11.84AMKK37 pKa = 10.3AFRR40 pKa = 11.84WALAKK45 pKa = 10.55RR46 pKa = 11.84NLGRR50 pKa = 11.84WGRR53 pKa = 11.84MVMRR57 pKa = 11.84SAARR61 pKa = 11.84FAQQ64 pKa = 3.56

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3961

0

3961

1184873

25

3198

299.1

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.33 ± 0.054

0.81 ± 0.011

4.859 ± 0.031

7.066 ± 0.045

3.868 ± 0.028

7.514 ± 0.041

2.236 ± 0.018

6.343 ± 0.035

5.065 ± 0.037

10.351 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.021

3.099 ± 0.024

4.45 ± 0.026

4.023 ± 0.025

5.929 ± 0.039

5.182 ± 0.03

5.286 ± 0.036

7.531 ± 0.035

1.195 ± 0.015

3.186 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski