Providencia phage vB_PreS-Stilesk

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B169|A0A7G5B169_9CAUD Uncharacterized protein OS=Providencia phage vB_PreS-Stilesk OX=2761110 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 5.05NLVTIASLFKK12 pKa = 10.4QWEE15 pKa = 4.28ADD17 pKa = 3.88VKK19 pKa = 8.84PHH21 pKa = 5.37VYY23 pKa = 10.9EE24 pKa = 4.36MFGEE28 pKa = 4.48CQDD31 pKa = 3.98SYY33 pKa = 11.26IEE35 pKa = 3.98SWMEE39 pKa = 4.25FLDD42 pKa = 4.72LPLARR47 pKa = 11.84DD48 pKa = 4.01QITSLQWAFAEE59 pKa = 4.47NYY61 pKa = 10.63LEE63 pKa = 4.14VAQYY67 pKa = 8.32TNSWAEE73 pKa = 3.79EE74 pKa = 3.97FSRR77 pKa = 11.84LLTLLGVEE85 pKa = 4.74LKK87 pKa = 10.79SYY89 pKa = 10.62DD90 pKa = 3.65CSGVDD95 pKa = 3.49IFGTANYY102 pKa = 10.09QYY104 pKa = 11.08VLEE107 pKa = 4.28RR108 pKa = 11.84HH109 pKa = 5.93GKK111 pKa = 6.31TLNGVYY117 pKa = 10.67SMPAKK122 pKa = 10.43NGEE125 pKa = 3.99PSRR128 pKa = 11.84DD129 pKa = 3.97DD130 pKa = 3.34ILEE133 pKa = 4.13CLFLEE138 pKa = 4.22YY139 pKa = 10.91SEE141 pKa = 6.26FEE143 pKa = 3.89EE144 pKa = 4.54CQNDD148 pKa = 3.17FRR150 pKa = 11.84YY151 pKa = 10.57YY152 pKa = 9.88CEE154 pKa = 5.16KK155 pKa = 10.74YY156 pKa = 10.45QLDD159 pKa = 3.9EE160 pKa = 4.72NEE162 pKa = 4.76AEE164 pKa = 4.51SKK166 pKa = 10.73SLYY169 pKa = 10.16DD170 pKa = 3.3QSEE173 pKa = 4.24KK174 pKa = 10.84LFQEE178 pKa = 4.46LPDD181 pKa = 4.48LFAHH185 pKa = 6.55DD186 pKa = 4.34TTLSTDD192 pKa = 4.16DD193 pKa = 3.98IVSGFADD200 pKa = 3.84IVWKK204 pKa = 9.27MM205 pKa = 3.33

Molecular weight: 23.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B178|A0A7G5B178_9CAUD Uncharacterized protein OS=Providencia phage vB_PreS-Stilesk OX=2761110 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.34SIPAYY7 pKa = 9.79YY8 pKa = 9.93IKK10 pKa = 10.75ALEE13 pKa = 4.14LAAASAGARR22 pKa = 11.84VGAFSRR28 pKa = 11.84LPDD31 pKa = 3.13INTPHH36 pKa = 7.19GYY38 pKa = 11.01LMRR41 pKa = 11.84SRR43 pKa = 11.84VAARR47 pKa = 11.84PNGGFWFTWFCNRR60 pKa = 11.84EE61 pKa = 4.02DD62 pKa = 3.7ASFAGMGGITAEE74 pKa = 4.21TLDD77 pKa = 3.61VLKK80 pKa = 10.2TRR82 pKa = 11.84IRR84 pKa = 11.84GAYY87 pKa = 8.48NRR89 pKa = 11.84QHH91 pKa = 7.04KK92 pKa = 10.61GGTSNRR98 pKa = 11.84AIEE101 pKa = 4.36LLRR104 pKa = 11.84KK105 pKa = 9.43RR106 pKa = 11.84ARR108 pKa = 11.84GG109 pKa = 3.38

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

18925

41

1435

259.2

28.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.168 ± 0.5

0.956 ± 0.145

6.499 ± 0.277

6.721 ± 0.265

3.736 ± 0.167

7.028 ± 0.35

1.802 ± 0.174

5.649 ± 0.138

5.807 ± 0.394

7.424 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.091 ± 0.118

4.264 ± 0.187

4.597 ± 0.391

3.831 ± 0.326

5.469 ± 0.198

5.765 ± 0.239

6.24 ± 0.263

6.769 ± 0.266

1.659 ± 0.15

3.524 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski