Amino acid dipepetide frequency for Lactococcus phage 4268

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.64AlaAla: 5.64 ± 1.823
0.441AlaCys: 0.441 ± 0.198
5.288AlaAsp: 5.288 ± 0.666
4.759AlaGlu: 4.759 ± 1.008
2.027AlaPhe: 2.027 ± 0.472
5.376AlaGly: 5.376 ± 0.941
0.793AlaHis: 0.793 ± 0.254
5.64AlaIle: 5.64 ± 0.72
5.376AlaLys: 5.376 ± 1.185
4.671AlaLeu: 4.671 ± 0.882
2.379AlaMet: 2.379 ± 0.46
3.525AlaAsn: 3.525 ± 0.512
1.763AlaPro: 1.763 ± 0.622
4.142AlaGln: 4.142 ± 1.308
1.674AlaArg: 1.674 ± 0.492
4.406AlaSer: 4.406 ± 0.696
4.406AlaThr: 4.406 ± 0.839
3.701AlaVal: 3.701 ± 0.581
0.969AlaTrp: 0.969 ± 0.335
3.261AlaTyr: 3.261 ± 0.488
0.0AlaXaa: 0.0 ± 0.0
Cys
0.264CysAla: 0.264 ± 0.167
0.264CysCys: 0.264 ± 0.168
0.176CysAsp: 0.176 ± 0.126
0.441CysGlu: 0.441 ± 0.222
0.264CysPhe: 0.264 ± 0.141
0.441CysGly: 0.441 ± 0.261
0.529CysHis: 0.529 ± 0.371
0.088CysIle: 0.088 ± 0.076
0.617CysLys: 0.617 ± 0.256
0.176CysLeu: 0.176 ± 0.127
0.088CysMet: 0.088 ± 0.09
0.176CysAsn: 0.176 ± 0.157
0.264CysPro: 0.264 ± 0.146
0.264CysGln: 0.264 ± 0.182
0.176CysArg: 0.176 ± 0.136
0.176CysSer: 0.176 ± 0.132
0.353CysThr: 0.353 ± 0.158
0.176CysVal: 0.176 ± 0.124
0.264CysTrp: 0.264 ± 0.191
0.088CysTyr: 0.088 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
3.525AspAla: 3.525 ± 0.679
0.264AspCys: 0.264 ± 0.205
3.79AspAsp: 3.79 ± 0.593
4.23AspGlu: 4.23 ± 0.904
4.142AspPhe: 4.142 ± 0.517
6.433AspGly: 6.433 ± 2.346
0.705AspHis: 0.705 ± 0.224
4.583AspIle: 4.583 ± 0.596
5.64AspLys: 5.64 ± 0.563
4.495AspLeu: 4.495 ± 0.781
2.115AspMet: 2.115 ± 0.487
3.261AspAsn: 3.261 ± 0.617
2.379AspPro: 2.379 ± 0.399
1.939AspGln: 1.939 ± 0.424
2.203AspArg: 2.203 ± 0.448
3.878AspSer: 3.878 ± 0.504
2.908AspThr: 2.908 ± 0.475
4.318AspVal: 4.318 ± 0.576
1.234AspTrp: 1.234 ± 0.322
2.468AspTyr: 2.468 ± 0.472
0.0AspXaa: 0.0 ± 0.0
Glu
4.23GluAla: 4.23 ± 1.033
0.353GluCys: 0.353 ± 0.188
3.173GluAsp: 3.173 ± 0.654
4.847GluGlu: 4.847 ± 0.785
2.556GluPhe: 2.556 ± 0.45
2.732GluGly: 2.732 ± 0.52
1.146GluHis: 1.146 ± 0.38
4.671GluIle: 4.671 ± 0.655
6.169GluLys: 6.169 ± 1.244
6.345GluLeu: 6.345 ± 0.947
1.851GluMet: 1.851 ± 0.436
3.261GluAsn: 3.261 ± 0.611
1.674GluPro: 1.674 ± 0.451
3.79GluGln: 3.79 ± 0.704
1.851GluArg: 1.851 ± 0.473
3.613GluSer: 3.613 ± 0.608
3.437GluThr: 3.437 ± 0.641
3.79GluVal: 3.79 ± 0.652
1.674GluTrp: 1.674 ± 0.423
2.291GluTyr: 2.291 ± 0.399
0.0GluXaa: 0.0 ± 0.0
Phe
2.556PheAla: 2.556 ± 0.534
0.705PheCys: 0.705 ± 0.213
3.085PheAsp: 3.085 ± 0.606
2.115PheGlu: 2.115 ± 0.575
2.115PhePhe: 2.115 ± 0.387
2.291PheGly: 2.291 ± 0.518
0.441PheHis: 0.441 ± 0.2
2.82PheIle: 2.82 ± 0.57
2.82PheLys: 2.82 ± 0.457
3.349PheLeu: 3.349 ± 0.606
1.146PheMet: 1.146 ± 0.409
2.732PheAsn: 2.732 ± 0.385
1.234PhePro: 1.234 ± 0.354
1.322PheGln: 1.322 ± 0.354
1.498PheArg: 1.498 ± 0.425
3.173PheSer: 3.173 ± 0.672
2.379PheThr: 2.379 ± 0.49
1.939PheVal: 1.939 ± 0.522
0.529PheTrp: 0.529 ± 0.248
1.763PheTyr: 1.763 ± 0.331
0.0PheXaa: 0.0 ± 0.0
Gly
3.79GlyAla: 3.79 ± 0.5
0.088GlyCys: 0.088 ± 0.09
4.142GlyAsp: 4.142 ± 1.031
2.82GlyGlu: 2.82 ± 0.524
3.966GlyPhe: 3.966 ± 0.549
4.583GlyGly: 4.583 ± 0.981
0.705GlyHis: 0.705 ± 0.31
6.081GlyIle: 6.081 ± 0.793
5.288GlyLys: 5.288 ± 0.677
4.847GlyLeu: 4.847 ± 0.613
1.939GlyMet: 1.939 ± 0.277
4.142GlyAsn: 4.142 ± 0.547
0.969GlyPro: 0.969 ± 0.239
3.966GlyGln: 3.966 ± 0.648
2.379GlyArg: 2.379 ± 0.544
4.935GlySer: 4.935 ± 0.875
6.257GlyThr: 6.257 ± 1.706
3.966GlyVal: 3.966 ± 0.704
1.322GlyTrp: 1.322 ± 0.379
3.173GlyTyr: 3.173 ± 0.394
0.0GlyXaa: 0.0 ± 0.0
His
1.058HisAla: 1.058 ± 0.348
0.0HisCys: 0.0 ± 0.0
1.146HisAsp: 1.146 ± 0.355
1.058HisGlu: 1.058 ± 0.314
0.353HisPhe: 0.353 ± 0.2
0.705HisGly: 0.705 ± 0.272
0.264HisHis: 0.264 ± 0.147
0.881HisIle: 0.881 ± 0.304
0.969HisLys: 0.969 ± 0.286
0.969HisLeu: 0.969 ± 0.332
0.441HisMet: 0.441 ± 0.173
1.058HisAsn: 1.058 ± 0.282
0.441HisPro: 0.441 ± 0.173
0.705HisGln: 0.705 ± 0.221
0.264HisArg: 0.264 ± 0.183
0.705HisSer: 0.705 ± 0.258
0.617HisThr: 0.617 ± 0.191
0.264HisVal: 0.264 ± 0.187
0.0HisTrp: 0.0 ± 0.0
0.617HisTyr: 0.617 ± 0.299
0.0HisXaa: 0.0 ± 0.0
Ile
4.935IleAla: 4.935 ± 0.686
0.264IleCys: 0.264 ± 0.164
5.464IleAsp: 5.464 ± 0.798
3.525IleGlu: 3.525 ± 0.615
1.939IlePhe: 1.939 ± 0.623
5.111IleGly: 5.111 ± 0.597
0.881IleHis: 0.881 ± 0.508
4.935IleIle: 4.935 ± 0.889
5.905IleLys: 5.905 ± 0.877
4.23IleLeu: 4.23 ± 0.783
1.498IleMet: 1.498 ± 0.356
6.698IleAsn: 6.698 ± 0.948
2.115IlePro: 2.115 ± 0.361
2.556IleGln: 2.556 ± 0.535
3.349IleArg: 3.349 ± 0.52
5.111IleSer: 5.111 ± 0.787
4.23IleThr: 4.23 ± 0.807
4.23IleVal: 4.23 ± 0.558
0.441IleTrp: 0.441 ± 0.155
2.556IleTyr: 2.556 ± 0.528
0.0IleXaa: 0.0 ± 0.0
Lys
6.522LysAla: 6.522 ± 1.612
0.441LysCys: 0.441 ± 0.245
5.64LysAsp: 5.64 ± 0.867
4.759LysGlu: 4.759 ± 0.913
3.261LysPhe: 3.261 ± 0.569
5.993LysGly: 5.993 ± 1.14
1.234LysHis: 1.234 ± 0.395
5.993LysIle: 5.993 ± 0.768
6.874LysLys: 6.874 ± 1.225
6.433LysLeu: 6.433 ± 1.044
2.291LysMet: 2.291 ± 0.448
6.522LysAsn: 6.522 ± 0.884
2.468LysPro: 2.468 ± 0.577
4.318LysGln: 4.318 ± 1.032
2.644LysArg: 2.644 ± 0.535
4.847LysSer: 4.847 ± 0.843
5.993LysThr: 5.993 ± 0.719
4.054LysVal: 4.054 ± 0.524
0.793LysTrp: 0.793 ± 0.262
2.996LysTyr: 2.996 ± 0.429
0.0LysXaa: 0.0 ± 0.0
Leu
5.552LeuAla: 5.552 ± 0.806
0.617LeuCys: 0.617 ± 0.258
4.759LeuAsp: 4.759 ± 0.793
4.847LeuGlu: 4.847 ± 0.924
1.763LeuPhe: 1.763 ± 0.533
3.966LeuGly: 3.966 ± 0.629
0.441LeuHis: 0.441 ± 0.159
5.376LeuIle: 5.376 ± 0.553
6.786LeuLys: 6.786 ± 0.854
5.288LeuLeu: 5.288 ± 1.001
2.556LeuMet: 2.556 ± 0.508
6.169LeuAsn: 6.169 ± 0.663
2.203LeuPro: 2.203 ± 0.41
3.173LeuGln: 3.173 ± 0.639
2.556LeuArg: 2.556 ± 0.595
7.491LeuSer: 7.491 ± 0.794
4.406LeuThr: 4.406 ± 0.651
4.23LeuVal: 4.23 ± 0.617
1.058LeuTrp: 1.058 ± 0.404
1.586LeuTyr: 1.586 ± 0.415
0.0LeuXaa: 0.0 ± 0.0
Met
2.82MetAla: 2.82 ± 0.454
0.0MetCys: 0.0 ± 0.0
1.146MetAsp: 1.146 ± 0.343
1.586MetGlu: 1.586 ± 0.521
0.969MetPhe: 0.969 ± 0.295
1.498MetGly: 1.498 ± 0.324
0.088MetHis: 0.088 ± 0.084
1.41MetIle: 1.41 ± 0.384
3.173MetLys: 3.173 ± 0.696
1.586MetLeu: 1.586 ± 0.436
1.234MetMet: 1.234 ± 0.361
1.851MetAsn: 1.851 ± 0.57
0.441MetPro: 0.441 ± 0.191
1.41MetGln: 1.41 ± 0.316
1.058MetArg: 1.058 ± 0.287
2.644MetSer: 2.644 ± 0.459
2.82MetThr: 2.82 ± 0.564
0.881MetVal: 0.881 ± 0.225
0.264MetTrp: 0.264 ± 0.149
0.617MetTyr: 0.617 ± 0.284
0.0MetXaa: 0.0 ± 0.0
Asn
3.966AsnAla: 3.966 ± 0.484
0.353AsnCys: 0.353 ± 0.234
4.23AsnAsp: 4.23 ± 0.818
3.79AsnGlu: 3.79 ± 0.428
2.379AsnPhe: 2.379 ± 0.499
5.464AsnGly: 5.464 ± 0.967
0.617AsnHis: 0.617 ± 0.239
4.23AsnIle: 4.23 ± 0.711
4.583AsnLys: 4.583 ± 0.802
5.2AsnLeu: 5.2 ± 0.691
1.674AsnMet: 1.674 ± 0.449
3.878AsnAsn: 3.878 ± 0.685
1.851AsnPro: 1.851 ± 0.467
2.468AsnGln: 2.468 ± 0.391
2.996AsnArg: 2.996 ± 0.599
5.464AsnSer: 5.464 ± 1.05
3.701AsnThr: 3.701 ± 0.561
3.349AsnVal: 3.349 ± 0.447
0.529AsnTrp: 0.529 ± 0.211
2.379AsnTyr: 2.379 ± 0.54
0.0AsnXaa: 0.0 ± 0.0
Pro
1.674ProAla: 1.674 ± 0.418
0.088ProCys: 0.088 ± 0.107
1.586ProAsp: 1.586 ± 0.383
1.674ProGlu: 1.674 ± 0.427
1.234ProPhe: 1.234 ± 0.437
1.41ProGly: 1.41 ± 0.562
0.441ProHis: 0.441 ± 0.185
1.498ProIle: 1.498 ± 0.313
1.498ProLys: 1.498 ± 0.346
1.763ProLeu: 1.763 ± 0.357
0.705ProMet: 0.705 ± 0.291
1.939ProAsn: 1.939 ± 0.557
0.617ProPro: 0.617 ± 0.305
1.498ProGln: 1.498 ± 0.398
0.793ProArg: 0.793 ± 0.25
2.468ProSer: 2.468 ± 0.603
2.732ProThr: 2.732 ± 0.888
1.586ProVal: 1.586 ± 0.462
0.441ProTrp: 0.441 ± 0.207
0.793ProTyr: 0.793 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
4.495GlnAla: 4.495 ± 0.619
0.088GlnCys: 0.088 ± 0.091
2.115GlnAsp: 2.115 ± 0.453
2.644GlnGlu: 2.644 ± 0.481
1.851GlnPhe: 1.851 ± 0.438
3.79GlnGly: 3.79 ± 0.601
0.529GlnHis: 0.529 ± 0.276
3.261GlnIle: 3.261 ± 0.566
4.583GlnLys: 4.583 ± 1.378
3.966GlnLeu: 3.966 ± 0.621
1.058GlnMet: 1.058 ± 0.426
1.851GlnAsn: 1.851 ± 0.427
0.617GlnPro: 0.617 ± 0.201
2.291GlnGln: 2.291 ± 0.44
1.234GlnArg: 1.234 ± 0.364
3.79GlnSer: 3.79 ± 0.676
2.644GlnThr: 2.644 ± 0.519
2.556GlnVal: 2.556 ± 0.388
0.529GlnTrp: 0.529 ± 0.228
2.115GlnTyr: 2.115 ± 0.417
0.0GlnXaa: 0.0 ± 0.0
Arg
1.498ArgAla: 1.498 ± 0.335
0.264ArgCys: 0.264 ± 0.171
2.291ArgAsp: 2.291 ± 0.436
3.437ArgGlu: 3.437 ± 0.904
1.939ArgPhe: 1.939 ± 0.404
2.556ArgGly: 2.556 ± 0.597
0.441ArgHis: 0.441 ± 0.25
1.763ArgIle: 1.763 ± 0.428
2.468ArgLys: 2.468 ± 0.534
2.027ArgLeu: 2.027 ± 0.398
0.881ArgMet: 0.881 ± 0.23
2.203ArgAsn: 2.203 ± 0.511
1.146ArgPro: 1.146 ± 0.325
1.586ArgGln: 1.586 ± 0.489
0.705ArgArg: 0.705 ± 0.313
2.203ArgSer: 2.203 ± 0.522
1.763ArgThr: 1.763 ± 0.437
1.763ArgVal: 1.763 ± 0.336
0.705ArgTrp: 0.705 ± 0.229
1.498ArgTyr: 1.498 ± 0.396
0.0ArgXaa: 0.0 ± 0.0
Ser
4.671SerAla: 4.671 ± 0.697
0.088SerCys: 0.088 ± 0.098
5.464SerAsp: 5.464 ± 0.822
5.552SerGlu: 5.552 ± 0.639
2.644SerPhe: 2.644 ± 0.344
5.464SerGly: 5.464 ± 0.888
1.322SerHis: 1.322 ± 0.243
2.996SerIle: 2.996 ± 0.495
5.905SerLys: 5.905 ± 0.855
5.817SerLeu: 5.817 ± 0.757
1.851SerMet: 1.851 ± 0.384
5.023SerAsn: 5.023 ± 0.567
1.322SerPro: 1.322 ± 0.309
2.908SerGln: 2.908 ± 0.548
2.468SerArg: 2.468 ± 0.477
5.817SerSer: 5.817 ± 0.782
5.288SerThr: 5.288 ± 1.115
4.935SerVal: 4.935 ± 0.517
1.41SerTrp: 1.41 ± 0.295
2.908SerTyr: 2.908 ± 0.702
0.0SerXaa: 0.0 ± 0.0
Thr
5.288ThrAla: 5.288 ± 1.019
0.264ThrCys: 0.264 ± 0.207
3.613ThrAsp: 3.613 ± 0.544
4.142ThrGlu: 4.142 ± 0.566
2.203ThrPhe: 2.203 ± 0.603
5.464ThrGly: 5.464 ± 0.742
0.793ThrHis: 0.793 ± 0.183
5.64ThrIle: 5.64 ± 0.687
5.464ThrLys: 5.464 ± 0.8
4.671ThrLeu: 4.671 ± 0.739
1.234ThrMet: 1.234 ± 0.337
3.966ThrAsn: 3.966 ± 0.998
1.851ThrPro: 1.851 ± 0.793
2.291ThrGln: 2.291 ± 0.688
2.115ThrArg: 2.115 ± 0.339
4.318ThrSer: 4.318 ± 1.142
5.111ThrThr: 5.111 ± 1.588
5.817ThrVal: 5.817 ± 1.264
0.881ThrTrp: 0.881 ± 0.378
1.939ThrTyr: 1.939 ± 0.58
0.0ThrXaa: 0.0 ± 0.0
Val
4.671ValAla: 4.671 ± 0.852
0.088ValCys: 0.088 ± 0.086
4.495ValAsp: 4.495 ± 0.671
4.495ValGlu: 4.495 ± 0.737
2.203ValPhe: 2.203 ± 0.608
2.996ValGly: 2.996 ± 0.402
0.353ValHis: 0.353 ± 0.163
4.054ValIle: 4.054 ± 0.512
5.552ValLys: 5.552 ± 0.778
4.935ValLeu: 4.935 ± 0.607
1.322ValMet: 1.322 ± 0.406
2.732ValAsn: 2.732 ± 0.792
2.115ValPro: 2.115 ± 0.517
2.027ValGln: 2.027 ± 0.438
1.41ValArg: 1.41 ± 0.465
4.318ValSer: 4.318 ± 0.753
3.878ValThr: 3.878 ± 0.788
3.701ValVal: 3.701 ± 0.655
0.793ValTrp: 0.793 ± 0.325
1.41ValTyr: 1.41 ± 0.322
0.0ValXaa: 0.0 ± 0.0
Trp
0.617TrpAla: 0.617 ± 0.195
0.088TrpCys: 0.088 ± 0.091
0.441TrpAsp: 0.441 ± 0.288
0.705TrpGlu: 0.705 ± 0.37
0.529TrpPhe: 0.529 ± 0.186
0.705TrpGly: 0.705 ± 0.213
0.176TrpHis: 0.176 ± 0.127
1.322TrpIle: 1.322 ± 0.404
1.322TrpLys: 1.322 ± 0.357
1.146TrpLeu: 1.146 ± 0.271
0.176TrpMet: 0.176 ± 0.129
0.969TrpAsn: 0.969 ± 0.425
0.0TrpPro: 0.0 ± 0.0
0.705TrpGln: 0.705 ± 0.245
0.881TrpArg: 0.881 ± 0.288
1.41TrpSer: 1.41 ± 0.515
1.498TrpThr: 1.498 ± 0.762
0.969TrpVal: 0.969 ± 0.31
0.264TrpTrp: 0.264 ± 0.189
0.705TrpTyr: 0.705 ± 0.253
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.468TyrAla: 2.468 ± 0.553
0.529TyrCys: 0.529 ± 0.261
2.468TyrAsp: 2.468 ± 0.347
2.027TyrGlu: 2.027 ± 0.372
1.674TyrPhe: 1.674 ± 0.428
2.379TyrGly: 2.379 ± 0.355
0.617TyrHis: 0.617 ± 0.244
2.82TyrIle: 2.82 ± 0.628
3.085TyrLys: 3.085 ± 0.503
2.908TyrLeu: 2.908 ± 0.806
0.969TyrMet: 0.969 ± 0.327
1.146TyrAsn: 1.146 ± 0.303
0.969TyrPro: 0.969 ± 0.37
2.644TyrGln: 2.644 ± 0.694
0.881TyrArg: 0.881 ± 0.266
3.085TyrSer: 3.085 ± 0.532
2.644TyrThr: 2.644 ± 0.875
1.322TyrVal: 1.322 ± 0.248
0.529TyrTrp: 0.529 ± 0.193
1.322TyrTyr: 1.322 ± 0.356
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (11348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski