Galinsoga mosaic virus (GaMV)
Average proteome isoelectric point is 7.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O12304|O12304_GAMV Capsid protein OS=Galinsoga mosaic virus OX=60714 PE=3 SV=1
MM1 pKa = 7.72 KK2 pKa = 10.29 YY3 pKa = 10.09 CRR5 pKa = 11.84 CSDD8 pKa = 3.73 TAPTDD13 pKa = 3.96 HH14 pKa = 6.62 ITLLFVIFILSGLILSLCTNITSNNYY40 pKa = 9.08 EE41 pKa = 3.76 NHH43 pKa = 4.44 TTEE46 pKa = 6.11 NKK48 pKa = 7.9 TQWITIGGQQQ58 pKa = 3.46
Molecular weight: 6.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.526
IPC2_protein 5.753
IPC_protein 5.715
Toseland 6.033
ProMoST 5.995
Dawson 6.02
Bjellqvist 5.982
Wikipedia 6.046
Rodwell 6.02
Grimsley 6.109
Solomon 6.033
Lehninger 6.033
Nozaki 6.287
DTASelect 6.478
Thurlkill 6.504
EMBOSS 6.478
Sillero 6.402
Patrickios 0.782
IPC_peptide 6.046
IPC2_peptide 6.402
IPC2.peptide.svr19 6.374
Protein with the highest isoelectric point:
>tr|O12302|O12302_GAMV Movement protein OS=Galinsoga mosaic virus OX=60714 PE=4 SV=1
MM1 pKa = 7.3 GAISKK6 pKa = 9.91 ISKK9 pKa = 9.44 ISSFVVAGIVAYY21 pKa = 9.54 KK22 pKa = 10.18 VGKK25 pKa = 8.47 ATYY28 pKa = 9.54 NAFRR32 pKa = 11.84 YY33 pKa = 9.63 RR34 pKa = 11.84 NQEE37 pKa = 4.06 SITAARR43 pKa = 11.84 DD44 pKa = 3.12 LQAAVEE50 pKa = 4.17 AVEE53 pKa = 4.15 EE54 pKa = 4.14 QPEE57 pKa = 4.19 FGSVEE62 pKa = 4.08 EE63 pKa = 4.67 CLEE66 pKa = 4.14 EE67 pKa = 4.45 TPASQSEE74 pKa = 4.15 RR75 pKa = 11.84 TTIQNEE81 pKa = 3.62 GDD83 pKa = 3.72 LAKK86 pKa = 10.23 MPVSRR91 pKa = 11.84 KK92 pKa = 8.8 RR93 pKa = 11.84 RR94 pKa = 11.84 IRR96 pKa = 11.84 NRR98 pKa = 11.84 KK99 pKa = 7.53 HH100 pKa = 5.53 GRR102 pKa = 11.84 FVSYY106 pKa = 10.43 LVNEE110 pKa = 4.73 AKK112 pKa = 10.67 AEE114 pKa = 4.02 YY115 pKa = 9.95 GLPKK119 pKa = 8.89 PTEE122 pKa = 4.26 AYY124 pKa = 10.2 RR125 pKa = 11.84 LMVGGFLNRR134 pKa = 11.84 LCKK137 pKa = 9.86 EE138 pKa = 3.83 WGVVTSHH145 pKa = 5.5 THH147 pKa = 3.93 QAVSIALPLVFVPTKK162 pKa = 10.18 FDD164 pKa = 3.44 VMGKK168 pKa = 10.22 AMCATTRR175 pKa = 11.84 TNQLIGEE182 pKa = 4.32 NSTDD186 pKa = 3.7 QATGWFDD193 pKa = 3.3 NVLGIGGGVGLRR205 pKa = 11.84 FLGKK209 pKa = 8.09 XGCLEE214 pKa = 3.78 RR215 pKa = 11.84 RR216 pKa = 11.84 LGFSTTVSRR225 pKa = 11.84 GSHH228 pKa = 6.85 PDD230 pKa = 3.46 LVVKK234 pKa = 10.26 PSGRR238 pKa = 11.84 PEE240 pKa = 3.84 KK241 pKa = 10.24 QRR243 pKa = 11.84 QLSAIVDD250 pKa = 3.31 SWGRR254 pKa = 11.84 KK255 pKa = 8.08 PLVFTITRR263 pKa = 11.84 LANLRR268 pKa = 11.84 RR269 pKa = 11.84 GMIEE273 pKa = 3.3 RR274 pKa = 11.84 MYY276 pKa = 10.87 YY277 pKa = 9.02 VEE279 pKa = 4.12 RR280 pKa = 11.84 DD281 pKa = 3.55 KK282 pKa = 11.82 NLVEE286 pKa = 4.43 PPAPSVDD293 pKa = 3.61 FRR295 pKa = 11.84 LPHH298 pKa = 6.49 FSSAFKK304 pKa = 10.62 KK305 pKa = 10.69 VIGRR309 pKa = 11.84 HH310 pKa = 4.38 TPVTRR315 pKa = 11.84 QAFVEE320 pKa = 5.42 FYY322 pKa = 10.23 RR323 pKa = 11.84 GRR325 pKa = 11.84 KK326 pKa = 6.22 RR327 pKa = 11.84 TIYY330 pKa = 9.69 EE331 pKa = 3.93 KK332 pKa = 10.72 AVEE335 pKa = 4.36 SLSAAPVTHH344 pKa = 7.16 RR345 pKa = 11.84 DD346 pKa = 3.17 ARR348 pKa = 11.84 LKK350 pKa = 10.3 TFVKK354 pKa = 10.53 AEE356 pKa = 3.98 KK357 pKa = 10.25 LVLSRR362 pKa = 11.84 KK363 pKa = 9.02 PDD365 pKa = 3.85 PAPRR369 pKa = 11.84 IIQPRR374 pKa = 11.84 APRR377 pKa = 11.84 YY378 pKa = 8.48 NVEE381 pKa = 3.83 LGVYY385 pKa = 9.83 LRR387 pKa = 11.84 PFEE390 pKa = 4.91 HH391 pKa = 6.62 KK392 pKa = 10.34 AFRR395 pKa = 11.84 AVEE398 pKa = 4.16 RR399 pKa = 11.84 VFGSPTIMKK408 pKa = 10.07 GYY410 pKa = 7.65 TAEE413 pKa = 4.13 EE414 pKa = 3.87 QASIARR420 pKa = 11.84 EE421 pKa = 3.49 KK422 pKa = 9.94 WDD424 pKa = 3.65 RR425 pKa = 11.84 FSKK428 pKa = 10.42 PVAIGLDD435 pKa = 3.26 ASRR438 pKa = 11.84 FDD440 pKa = 3.42 QHH442 pKa = 8.35 VSVAALKK449 pKa = 9.67 WEE451 pKa = 4.21 HH452 pKa = 6.55 KK453 pKa = 10.07 LYY455 pKa = 10.78 HH456 pKa = 6.4 HH457 pKa = 7.42 AFNHH461 pKa = 6.58 DD462 pKa = 3.36 KK463 pKa = 10.22 TLKK466 pKa = 10.64 KK467 pKa = 10.04 LLKK470 pKa = 9.06 WQIHH474 pKa = 5.14 NEE476 pKa = 3.9 GTAFASDD483 pKa = 3.43 GCIKK487 pKa = 10.48 YY488 pKa = 10.38 KK489 pKa = 9.74 KK490 pKa = 8.03 TGCRR494 pKa = 11.84 MSGDD498 pKa = 3.79 MNTSLGNCLIMCGMVYY514 pKa = 10.81 DD515 pKa = 4.86 LLVTHH520 pKa = 7.55 DD521 pKa = 4.55 IDD523 pKa = 6.31 AEE525 pKa = 4.19 LLNNGDD531 pKa = 4.17 DD532 pKa = 3.85 CVLICEE538 pKa = 4.46 RR539 pKa = 11.84 SEE541 pKa = 3.9 EE542 pKa = 3.99 AHH544 pKa = 6.44 LLEE547 pKa = 5.83 VIPQYY552 pKa = 10.48 FRR554 pKa = 11.84 QYY556 pKa = 9.4 GFTMEE561 pKa = 4.15 VEE563 pKa = 4.34 KK564 pKa = 10.42 PVHH567 pKa = 6.33 IFEE570 pKa = 4.97 KK571 pKa = 11.15 LEE573 pKa = 3.99 FCQTQPVYY581 pKa = 11.36 VDD583 pKa = 3.83 GKK585 pKa = 8.93 WIMVRR590 pKa = 11.84 KK591 pKa = 8.97 PNPAMSKK598 pKa = 10.73 DD599 pKa = 3.92 LTCLLPIQNEE609 pKa = 4.31 NNFKK613 pKa = 9.82 EE614 pKa = 4.27 WLSAVSEE621 pKa = 4.44 CGLALTGGVPLWQNFYY637 pKa = 9.82 QACNLGAARR646 pKa = 11.84 TGFTKK651 pKa = 10.49 SGLFTEE657 pKa = 4.98 SGLAYY662 pKa = 10.23 ASRR665 pKa = 11.84 GLHH668 pKa = 5.58 RR669 pKa = 11.84 RR670 pKa = 11.84 FSEE673 pKa = 4.14 PSAEE677 pKa = 4.24 TRR679 pKa = 11.84 HH680 pKa = 5.3 SFYY683 pKa = 10.76 LAFGITPDD691 pKa = 3.7 LQLALEE697 pKa = 4.78 KK698 pKa = 10.75 EE699 pKa = 4.31 LDD701 pKa = 3.79 SKK703 pKa = 11.32 IMNEE707 pKa = 3.32 WTMIPRR713 pKa = 11.84 DD714 pKa = 3.61 TRR716 pKa = 11.84 QRR718 pKa = 11.84 TYY720 pKa = 11.12 YY721 pKa = 9.55 STISNEE727 pKa = 3.97 LTT729 pKa = 3.32
Molecular weight: 82.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.746
IPC2_protein 8.609
IPC_protein 8.492
Toseland 9.136
ProMoST 9.033
Dawson 9.487
Bjellqvist 9.326
Wikipedia 9.648
Rodwell 9.692
Grimsley 9.516
Solomon 9.516
Lehninger 9.472
Nozaki 9.472
DTASelect 9.224
Thurlkill 9.355
EMBOSS 9.633
Sillero 9.502
Patrickios 4.838
IPC_peptide 9.502
IPC2_peptide 8.053
IPC2.peptide.svr19 7.736
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1405
58
729
281.0
31.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.473 ± 0.932
1.637 ± 0.398
3.63 ± 0.291
5.694 ± 1.314
4.626 ± 0.293
7.758 ± 0.787
2.206 ± 0.36
4.555 ± 0.709
5.338 ± 0.94
7.829 ± 0.896
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.993 ± 0.504
5.125 ± 1.551
4.555 ± 0.536
4.342 ± 0.851
6.406 ± 0.926
7.331 ± 0.936
7.829 ± 1.565
7.26 ± 0.624
1.139 ± 0.184
3.203 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here