Pseudomonas hussainii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7JZR0|A0A1H7JZR0_9PSED Cytochrome c-type biogenesis protein CcmH OS=Pseudomonas hussainii OX=1429083 GN=SAMN05216214_105103 PE=4 SV=1
MM1 pKa = 7.22NALYY5 pKa = 10.32YY6 pKa = 9.28LIPIAIVLVIAAIWLFFWAVDD27 pKa = 3.24SGQYY31 pKa = 10.95DD32 pKa = 4.11DD33 pKa = 5.98LDD35 pKa = 4.98GPAHH39 pKa = 6.9SILFDD44 pKa = 4.62DD45 pKa = 5.27EE46 pKa = 5.85DD47 pKa = 4.3PAHH50 pKa = 6.18TAAQKK55 pKa = 8.65TAQSTDD61 pKa = 3.26NNPKK65 pKa = 10.46PNDD68 pKa = 3.4

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7FP66|A0A1H7FP66_9PSED Phosphate-specific transport system accessory protein PhoU OS=Pseudomonas hussainii OX=1429083 GN=SAMN05216214_101269 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.42NGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3415

0

3415

1111681

29

3553

325.5

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.42 ± 0.053

1.087 ± 0.016

5.175 ± 0.036

5.89 ± 0.042

3.526 ± 0.026

7.524 ± 0.042

2.447 ± 0.024

4.458 ± 0.03

3.473 ± 0.036

12.275 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.018

3.08 ± 0.034

4.719 ± 0.029

5.355 ± 0.044

6.196 ± 0.04

5.769 ± 0.035

4.481 ± 0.032

6.84 ± 0.042

1.501 ± 0.02

2.555 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski