Rousettus aegyptiacus polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S7J032|A0A1S7J032_9POLY Minor capsid protein OS=Rousettus aegyptiacus polyomavirus 1 OX=1904411 PE=3 SV=1
MM1 pKa = 7.31GALLAVLAEE10 pKa = 4.15VFEE13 pKa = 4.66ISAATGFTVDD23 pKa = 4.99SILTGEE29 pKa = 4.41ALVADD34 pKa = 5.08EE35 pKa = 5.43LLQAYY40 pKa = 7.15VTNLVTLEE48 pKa = 4.11GFTEE52 pKa = 4.83GEE54 pKa = 3.88ALLAIGFSPEE64 pKa = 3.46AAQLLTSLAPTFPEE78 pKa = 4.41AFTLVAGTEE87 pKa = 4.17ALVHH91 pKa = 6.19GSLFIGAATGAALAPYY107 pKa = 8.99SWDD110 pKa = 3.37YY111 pKa = 9.65ATPIADD117 pKa = 4.24LNQHH121 pKa = 6.35LMALQVWQPDD131 pKa = 4.11LNWEE135 pKa = 4.05DD136 pKa = 4.19VYY138 pKa = 11.37FPGVLPFARR147 pKa = 11.84FVNYY151 pKa = 9.56IDD153 pKa = 3.88PANWASNLYY162 pKa = 9.65HH163 pKa = 7.6AIGRR167 pKa = 11.84YY168 pKa = 7.45FWEE171 pKa = 4.21TAQRR175 pKa = 11.84AGTRR179 pKa = 11.84LIEE182 pKa = 3.87QEE184 pKa = 4.14VRR186 pKa = 11.84HH187 pKa = 5.78VSTDD191 pKa = 3.12LAQRR195 pKa = 11.84TVTSIAEE202 pKa = 3.8TLARR206 pKa = 11.84YY207 pKa = 9.22FEE209 pKa = 4.06NARR212 pKa = 11.84WAVSEE217 pKa = 4.48VTSGTYY223 pKa = 10.04GALQQYY229 pKa = 9.88YY230 pKa = 10.36SEE232 pKa = 5.11LPPLRR237 pKa = 11.84PPQIRR242 pKa = 11.84ALHH245 pKa = 5.79KK246 pKa = 10.64RR247 pKa = 11.84LGEE250 pKa = 4.3DD251 pKa = 3.06APSRR255 pKa = 11.84YY256 pKa = 10.41SFDD259 pKa = 4.06SNGNGSAQYY268 pKa = 10.32VDD270 pKa = 5.1KK271 pKa = 11.41VDD273 pKa = 3.61APGGARR279 pKa = 11.84QRR281 pKa = 11.84HH282 pKa = 4.9TPDD285 pKa = 2.34WMLPLILGLYY295 pKa = 9.58GDD297 pKa = 5.31IYY299 pKa = 10.35PSWEE303 pKa = 3.8ATLEE307 pKa = 3.98EE308 pKa = 4.93LEE310 pKa = 4.76AEE312 pKa = 3.99EE313 pKa = 5.87DD314 pKa = 4.02GPKK317 pKa = 9.98KK318 pKa = 10.3KK319 pKa = 10.28KK320 pKa = 9.6PRR322 pKa = 11.84SEE324 pKa = 3.67GRR326 pKa = 11.84RR327 pKa = 11.84TRR329 pKa = 11.84GRR331 pKa = 11.84KK332 pKa = 8.63SQSSSS337 pKa = 2.78

Molecular weight: 37.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S7J030|A0A1S7J030_9POLY Minor capsid protein OS=Rousettus aegyptiacus polyomavirus 1 OX=1904411 PE=3 SV=1
MM1 pKa = 7.25GHH3 pKa = 6.05QNGINGGLTLMTCSAMNIFLQMKK26 pKa = 9.61RR27 pKa = 11.84NPLHH31 pKa = 6.1FRR33 pKa = 11.84RR34 pKa = 11.84EE35 pKa = 4.15NHH37 pKa = 5.76HH38 pKa = 6.36HH39 pKa = 6.08QSLHH43 pKa = 4.94KK44 pKa = 8.63QHH46 pKa = 5.93RR47 pKa = 11.84QKK49 pKa = 10.51RR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 9.03LHH54 pKa = 5.64PPKK57 pKa = 10.37IFLKK61 pKa = 9.92TFSPSS66 pKa = 2.72

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1806

66

652

301.0

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.7 ± 1.646

1.993 ± 0.795

4.817 ± 0.405

6.977 ± 0.555

4.43 ± 0.553

6.811 ± 0.883

2.658 ± 0.658

3.987 ± 0.525

6.257 ± 1.356

10.465 ± 0.824

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.483

4.319 ± 0.471

6.035 ± 0.416

4.374 ± 0.358

4.817 ± 0.808

6.589 ± 0.141

5.759 ± 0.424

5.205 ± 0.771

1.661 ± 0.531

4.042 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski