Hubei rhabdo-like virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Mymonaviridae; Hubramonavirus; Hubramonavirus hubeiense

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMJ0|A0A1L3KMJ0_9MONO Uncharacterized protein OS=Hubei rhabdo-like virus 4 OX=1923188 PE=4 SV=1
MM1 pKa = 7.89PSTYY5 pKa = 11.01SKK7 pKa = 10.81FFAPKK12 pKa = 8.78IAHH15 pKa = 6.31GGVKK19 pKa = 10.24GKK21 pKa = 10.41RR22 pKa = 11.84LVKK25 pKa = 10.37SAISSAIPVPVLPTSIPTEE44 pKa = 3.72DD45 pKa = 3.25RR46 pKa = 11.84LRR48 pKa = 11.84YY49 pKa = 9.09LSAMASLGTSLTGTVASWKK68 pKa = 10.39VYY70 pKa = 9.45ILAYY74 pKa = 9.22TAMLFPSLEE83 pKa = 3.84KK84 pKa = 10.72AFPRR88 pKa = 11.84EE89 pKa = 3.62IYY91 pKa = 10.6APIAIPPEE99 pKa = 4.08TVTAVLGAFNGLEE112 pKa = 4.04AMPEE116 pKa = 4.03EE117 pKa = 4.5YY118 pKa = 10.36DD119 pKa = 3.99QEE121 pKa = 4.27EE122 pKa = 4.17LHH124 pKa = 6.09EE125 pKa = 4.88AEE127 pKa = 4.26VAFNKK132 pKa = 10.44LSLGPGLPMGSSLMKK147 pKa = 10.16TRR149 pKa = 11.84FLPEE153 pKa = 3.18EE154 pKa = 3.97AAAKK158 pKa = 10.19VIASHH163 pKa = 6.14YY164 pKa = 10.55SVLLFIAGKK173 pKa = 10.35RR174 pKa = 11.84IDD176 pKa = 4.5SEE178 pKa = 4.23DD179 pKa = 3.39HH180 pKa = 6.43SALTRR185 pKa = 11.84NRR187 pKa = 11.84PDD189 pKa = 3.08ALIRR193 pKa = 11.84KK194 pKa = 8.4AHH196 pKa = 6.13MEE198 pKa = 4.01QEE200 pKa = 4.28PQLLTGNLRR209 pKa = 11.84MSDD212 pKa = 3.21KK213 pKa = 10.99SHH215 pKa = 7.2NEE217 pKa = 3.8LNSAWQEE224 pKa = 3.69LAALRR229 pKa = 11.84GVIFPTFAEE238 pKa = 4.99FAADD242 pKa = 3.64TTDD245 pKa = 3.21EE246 pKa = 4.3VQDD249 pKa = 4.54LIYY252 pKa = 8.6TTMHH256 pKa = 7.44LLRR259 pKa = 11.84FSGMQHH265 pKa = 5.76AKK267 pKa = 9.58IVHH270 pKa = 6.13GFLGAFPWARR280 pKa = 11.84EE281 pKa = 4.1VPALRR286 pKa = 11.84SAIAKK291 pKa = 9.7FDD293 pKa = 3.82EE294 pKa = 4.69SVVAAAGYY302 pKa = 7.83PIHH305 pKa = 6.36IQPYY309 pKa = 7.87VKK311 pKa = 10.47LIYY314 pKa = 10.17GDD316 pKa = 3.41KK317 pKa = 11.39AEE319 pKa = 4.21IFPRR323 pKa = 11.84KK324 pKa = 9.0EE325 pKa = 3.67MEE327 pKa = 4.11VLISCAVAASQEE339 pKa = 4.11TSPDD343 pKa = 2.7IVNFYY348 pKa = 9.86STDD351 pKa = 3.09AFSPIVEE358 pKa = 4.07AFMTEE363 pKa = 4.06RR364 pKa = 11.84EE365 pKa = 4.16RR366 pKa = 11.84RR367 pKa = 11.84GHH369 pKa = 6.04VRR371 pKa = 11.84TLTLRR376 pKa = 11.84AQEE379 pKa = 4.39ASLKK383 pKa = 10.5SKK385 pKa = 10.5IGITGGEE392 pKa = 4.0QGSDD396 pKa = 3.4EE397 pKa = 4.14EE398 pKa = 4.92DD399 pKa = 3.29EE400 pKa = 4.55EE401 pKa = 4.29EE402 pKa = 4.76FYY404 pKa = 11.0QIPEE408 pKa = 4.17EE409 pKa = 4.02EE410 pKa = 5.1HH411 pKa = 6.23EE412 pKa = 5.01DD413 pKa = 3.53EE414 pKa = 6.63DD415 pKa = 4.57IAAA418 pKa = 4.59

Molecular weight: 46.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMM2|A0A1L3KMM2_9MONO Large structural protein OS=Hubei rhabdo-like virus 4 OX=1923188 PE=4 SV=1
MM1 pKa = 7.67TDD3 pKa = 2.92KK4 pKa = 10.87QAANAGMLPPPEE16 pKa = 3.98VSEE19 pKa = 4.53KK20 pKa = 10.38IEE22 pKa = 4.61SIASALPEE30 pKa = 3.95IDD32 pKa = 4.02FNAAAALLPSNTRR45 pKa = 11.84GIPPSSKK52 pKa = 10.57KK53 pKa = 9.93KK54 pKa = 10.31GKK56 pKa = 9.92QISPQSHH63 pKa = 6.59AATEE67 pKa = 4.5VEE69 pKa = 4.78SSRR72 pKa = 11.84QTPIPAGPKK81 pKa = 9.41VGASGHH87 pKa = 6.13SSTQRR92 pKa = 11.84SARR95 pKa = 11.84GVSRR99 pKa = 11.84GRR101 pKa = 11.84VRR103 pKa = 11.84PSRR106 pKa = 11.84QVFPPRR112 pKa = 11.84KK113 pKa = 8.31LASVFQEE120 pKa = 3.58VDD122 pKa = 3.29EE123 pKa = 5.45ADD125 pKa = 3.77LPEE128 pKa = 4.62PTLADD133 pKa = 3.97PVTSDD138 pKa = 3.24QIDD141 pKa = 3.51RR142 pKa = 11.84MSEE145 pKa = 3.77VSSRR149 pKa = 11.84MQEE152 pKa = 3.98MIQDD156 pKa = 3.7HH157 pKa = 6.6EE158 pKa = 5.09DD159 pKa = 3.7IINSLAEE166 pKa = 3.8RR167 pKa = 11.84LEE169 pKa = 4.01NMEE172 pKa = 4.04KK173 pKa = 10.41SYY175 pKa = 11.43GILLTKK181 pKa = 9.09HH182 pKa = 5.87TNLQKK187 pKa = 10.86EE188 pKa = 4.26CDD190 pKa = 3.7GLKK193 pKa = 10.46VQVEE197 pKa = 4.16ANKK200 pKa = 10.36RR201 pKa = 11.84GPQAEE206 pKa = 4.1LRR208 pKa = 11.84LGGSNSLTNTPFSSSTAGSSSILNRR233 pKa = 11.84PGFEE237 pKa = 3.98QAPKK241 pKa = 8.71PTIEE245 pKa = 4.67GGTDD249 pKa = 3.1SMVAGQHH256 pKa = 4.11RR257 pKa = 11.84RR258 pKa = 11.84RR259 pKa = 11.84RR260 pKa = 11.84AKK262 pKa = 10.18YY263 pKa = 9.18

Molecular weight: 28.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2630

263

1949

876.7

97.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.985 ± 1.56

1.331 ± 0.541

5.019 ± 0.474

6.502 ± 1.32

3.612 ± 0.486

6.464 ± 0.317

2.281 ± 0.194

5.97 ± 0.263

4.335 ± 0.455

10.266 ± 1.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.036

2.738 ± 0.375

5.551 ± 0.876

3.042 ± 0.672

6.692 ± 0.637

9.392 ± 0.762

5.133 ± 0.158

6.312 ± 0.548

1.217 ± 0.428

3.384 ± 0.748
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski