Streptococcus phage Javan306
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AZ04|A0A4D6AZ04_9CAUD Uncharacterized protein OS=Streptococcus phage Javan306 OX=2548099 GN=Javan306_0031 PE=4 SV=1
MM1 pKa = 8.1 AYY3 pKa = 10.01 QVIRR7 pKa = 11.84 PFKK10 pKa = 10.32 DD11 pKa = 3.11 LRR13 pKa = 11.84 DD14 pKa = 3.88 PQQYY18 pKa = 9.81 EE19 pKa = 4.0 YY20 pKa = 10.82 QIGDD24 pKa = 3.73 IYY26 pKa = 10.67 PRR28 pKa = 11.84 TGYY31 pKa = 10.16 RR32 pKa = 11.84 SSKK35 pKa = 11.04 AFIQEE40 pKa = 4.26 LLDD43 pKa = 3.99 GSNSAGSIFLTKK55 pKa = 9.68 IDD57 pKa = 5.11 DD58 pKa = 4.18 DD59 pKa = 5.27 DD60 pKa = 4.75 ISEE63 pKa = 4.8 GEE65 pKa = 4.56 AEE67 pKa = 4.17 TQEE70 pKa = 4.19 PEE72 pKa = 4.38 EE73 pKa = 4.37 EE74 pKa = 4.22 DD75 pKa = 3.69 EE76 pKa = 4.32 EE77 pKa = 4.47
Molecular weight: 8.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.186
IPC2_protein 3.961
IPC_protein 3.884
Toseland 3.694
ProMoST 3.986
Dawson 3.859
Bjellqvist 4.062
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.617
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 3.999
Patrickios 3.427
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|A0A4D6AYF4|A0A4D6AYF4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan306 OX=2548099 GN=Javan306_0032 PE=4 SV=1
MM1 pKa = 7.02 YY2 pKa = 9.35 NKK4 pKa = 9.94 PVRR7 pKa = 11.84 QSLKK11 pKa = 7.46 TRR13 pKa = 11.84 KK14 pKa = 8.55 WYY16 pKa = 10.4 KK17 pKa = 10.33 FRR19 pKa = 11.84 DD20 pKa = 3.52 KK21 pKa = 11.37 VMRR24 pKa = 11.84 QYY26 pKa = 11.62 DD27 pKa = 4.06 YY28 pKa = 11.6 LCQEE32 pKa = 4.33 SLRR35 pKa = 11.84 YY36 pKa = 8.47 GQSVPAEE43 pKa = 4.42 MVHH46 pKa = 6.75 HH47 pKa = 7.39 IYY49 pKa = 10.05 PVSEE53 pKa = 4.23 YY54 pKa = 10.47 PEE56 pKa = 4.33 LEE58 pKa = 4.17 YY59 pKa = 11.24 VSWNCLPLTNRR70 pKa = 11.84 KK71 pKa = 9.47 HH72 pKa = 5.18 NTFHH76 pKa = 7.57 DD77 pKa = 3.98 RR78 pKa = 11.84 NNDD81 pKa = 3.69 KK82 pKa = 10.87 IIGNGIYY89 pKa = 8.6 WQKK92 pKa = 10.66 KK93 pKa = 6.62 RR94 pKa = 11.84 KK95 pKa = 9.72 KK96 pKa = 10.15 EE97 pKa = 3.57 FLTFFKK103 pKa = 11.02 NKK105 pKa = 9.59 NEE107 pKa = 4.11 KK108 pKa = 10.16
Molecular weight: 13.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.157
IPC2_protein 9.268
IPC_protein 9.209
Toseland 9.794
ProMoST 9.604
Dawson 10.058
Bjellqvist 9.75
Wikipedia 10.233
Rodwell 10.438
Grimsley 10.131
Solomon 10.087
Lehninger 10.043
Nozaki 9.823
DTASelect 9.736
Thurlkill 9.882
EMBOSS 10.218
Sillero 9.97
Patrickios 7.556
IPC_peptide 10.072
IPC2_peptide 8.39
IPC2.peptide.svr19 8.271
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
9837
48
1393
192.9
21.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.821 ± 0.528
0.6 ± 0.126
5.967 ± 0.362
8.478 ± 0.43
4.381 ± 0.373
6.313 ± 0.374
1.372 ± 0.194
6.994 ± 0.245
8.57 ± 0.391
8.346 ± 0.373
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.541 ± 0.2
5.581 ± 0.28
2.765 ± 0.23
3.904 ± 0.251
4.422 ± 0.442
5.977 ± 0.465
5.611 ± 0.415
6.069 ± 0.293
1.454 ± 0.173
3.832 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here