Perch rhabdovirus (isolate Perch/France/PRV/1981)
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7X6R3|K7X6R3_PRVF Matrix protein OS=Perch rhabdovirus (isolate Perch/France/PRV/1981) OX=1559359 GN=M PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 5.15 AGAIVVRR9 pKa = 11.84 STKK12 pKa = 10.68 ASFVPLQPQLEE23 pKa = 4.27 DD24 pKa = 4.16 EE25 pKa = 4.49 INYY28 pKa = 9.24 PADD31 pKa = 3.34 FFVNQKK37 pKa = 9.32 IPTFVRR43 pKa = 11.84 YY44 pKa = 9.43 YY45 pKa = 10.87 EE46 pKa = 4.01 NAKK49 pKa = 10.68 PEE51 pKa = 4.54 DD52 pKa = 3.99 LLGMIFGEE60 pKa = 4.38 LSEE63 pKa = 4.5 SRR65 pKa = 11.84 LPSEE69 pKa = 5.05 LVTSYY74 pKa = 10.33 IYY76 pKa = 11.15 SVMSKK81 pKa = 7.22 WTEE84 pKa = 3.82 TLDD87 pKa = 4.43 APWEE91 pKa = 4.22 SFGIEE96 pKa = 3.94 FGDD99 pKa = 3.86 VNDD102 pKa = 3.68 ILTPFSLLKK111 pKa = 10.83 CIVSDD116 pKa = 4.89 QALADD121 pKa = 3.98 YY122 pKa = 8.23 KK123 pKa = 10.48 TVAVPGDD130 pKa = 3.65 IDD132 pKa = 5.05 PIAMMIFLLAPYY144 pKa = 9.64 RR145 pKa = 11.84 IVGIQNEE152 pKa = 4.61 EE153 pKa = 3.78 YY154 pKa = 9.16 QARR157 pKa = 11.84 VIQTIQNQIDD167 pKa = 3.66 SLGAKK172 pKa = 9.37 RR173 pKa = 11.84 LNVRR177 pKa = 11.84 SLKK180 pKa = 10.89 NITTLTRR187 pKa = 11.84 SPNYY191 pKa = 9.72 LKK193 pKa = 10.81 LVAAIDD199 pKa = 3.28 MFYY202 pKa = 11.09 FHH204 pKa = 7.49 FKK206 pKa = 10.44 NSQEE210 pKa = 4.06 RR211 pKa = 11.84 AVVRR215 pKa = 11.84 VATLGSRR222 pKa = 11.84 HH223 pKa = 6.47 KK224 pKa = 10.91 DD225 pKa = 3.16 CAALSTLNHH234 pKa = 6.08 IVQFTGKK241 pKa = 9.39 PLIEE245 pKa = 4.01 VLDD248 pKa = 3.82 WVFTDD253 pKa = 3.94 QVAQEE258 pKa = 4.51 VSRR261 pKa = 11.84 MMRR264 pKa = 11.84 PGQEE268 pKa = 3.22 IDD270 pKa = 3.38 QADD273 pKa = 3.8 SYY275 pKa = 11.27 MPYY278 pKa = 10.71 LKK280 pKa = 10.68 DD281 pKa = 4.11 LGLCRR286 pKa = 11.84 KK287 pKa = 9.59 SPYY290 pKa = 9.96 SSSANPGVHH299 pKa = 6.14 CWAQMTCALLGSKK312 pKa = 10.24 RR313 pKa = 11.84 SQNAIASTEE322 pKa = 4.0 EE323 pKa = 3.69 NLVNLTRR330 pKa = 11.84 NAEE333 pKa = 3.59 IMAYY337 pKa = 10.19 VLGTGAVLVKK347 pKa = 10.28 ALEE350 pKa = 4.1 IGGVKK355 pKa = 9.26 GTDD358 pKa = 3.37 PDD360 pKa = 5.57 DD361 pKa = 3.37 ITVDD365 pKa = 3.06 VDD367 pKa = 3.82 GAGEE371 pKa = 4.15 PTTLEE376 pKa = 4.0 ALDD379 pKa = 3.75 WLDD382 pKa = 4.09 FVQTNGCQLTPKK394 pKa = 9.12 MEE396 pKa = 4.16 AKK398 pKa = 9.84 IRR400 pKa = 11.84 VMSLRR405 pKa = 11.84 IQNARR410 pKa = 11.84 KK411 pKa = 8.73 DD412 pKa = 3.73 TIGAYY417 pKa = 10.33 LKK419 pKa = 10.82 GRR421 pKa = 11.84 AVQNEE426 pKa = 3.91
Molecular weight: 47.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.997
IPC2_protein 5.067
IPC_protein 5.003
Toseland 4.889
ProMoST 5.067
Dawson 4.965
Bjellqvist 5.13
Wikipedia 4.851
Rodwell 4.876
Grimsley 4.8
Solomon 4.965
Lehninger 4.927
Nozaki 5.08
DTASelect 5.258
Thurlkill 4.889
EMBOSS 4.876
Sillero 5.143
Patrickios 4.037
IPC_peptide 4.978
IPC2_peptide 5.143
IPC2.peptide.svr19 5.128
Protein with the highest isoelectric point:
>tr|K7X7F6|K7X7F6_PRVF GDP polyribonucleotidyltransferase OS=Perch rhabdovirus (isolate Perch/France/PRV/1981) OX=1559359 GN=L PE=4 SV=1
MM1 pKa = 7.71 ALNIFKK7 pKa = 10.82 KK8 pKa = 10.06 KK9 pKa = 10.24 GKK11 pKa = 9.4 SNKK14 pKa = 9.77 EE15 pKa = 3.66 MDD17 pKa = 3.61 SGKK20 pKa = 10.63 ALAMPSAPPVYY31 pKa = 10.31 SEE33 pKa = 4.85 APGPFDD39 pKa = 3.27 AWNTSSIEE47 pKa = 4.03 EE48 pKa = 4.12 LSKK51 pKa = 11.08 VSYY54 pKa = 10.78 LVDD57 pKa = 3.19 TCLSVTTKK65 pKa = 10.94 GPLQSVADD73 pKa = 4.41 AYY75 pKa = 10.33 MVAQGMMDD83 pKa = 4.12 YY84 pKa = 11.32 YY85 pKa = 11.06 NGPVLSKK92 pKa = 10.36 PFFMTLFLGGVHH104 pKa = 6.77 GMQASVRR111 pKa = 11.84 GSQSIRR117 pKa = 11.84 YY118 pKa = 7.89 EE119 pKa = 4.23 RR120 pKa = 11.84 EE121 pKa = 3.23 FHH123 pKa = 7.06 GPMTFPYY130 pKa = 9.82 HH131 pKa = 6.93 KK132 pKa = 10.17 SNPVDD137 pKa = 3.37 WTPKK141 pKa = 9.6 SVSVHH146 pKa = 4.49 YY147 pKa = 9.69 TSSLRR152 pKa = 11.84 GKK154 pKa = 9.17 AVEE157 pKa = 4.35 VVFEE161 pKa = 4.43 ARR163 pKa = 11.84 LKK165 pKa = 9.6 STRR168 pKa = 11.84 QMGPSVWHH176 pKa = 6.09 YY177 pKa = 11.64 LDD179 pKa = 3.68 GLKK182 pKa = 10.73 GIQRR186 pKa = 11.84 PGNEE190 pKa = 3.68 AVLKK194 pKa = 10.1 QFGVPTMMLGNNLVFNLDD212 pKa = 3.34 VQMSDD217 pKa = 3.22 LL218 pKa = 4.06
Molecular weight: 24.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.502
IPC2_protein 8.463
IPC_protein 8.434
Toseland 9.019
ProMoST 8.829
Dawson 9.311
Bjellqvist 9.063
Wikipedia 9.487
Rodwell 9.487
Grimsley 9.385
Solomon 9.414
Lehninger 9.37
Nozaki 9.077
DTASelect 9.033
Thurlkill 9.136
EMBOSS 9.443
Sillero 9.268
Patrickios 4.533
IPC_peptide 9.399
IPC2_peptide 7.673
IPC2.peptide.svr19 7.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3579
218
2097
715.8
81.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.393 ± 0.525
1.369 ± 0.3
5.644 ± 0.237
6.901 ± 0.45
4.135 ± 0.107
6.315 ± 0.461
2.431 ± 0.297
6.175 ± 0.599
6.566 ± 0.433
9.5 ± 0.604
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.822 ± 0.425
4.582 ± 0.171
4.806 ± 0.847
3.688 ± 0.231
5.225 ± 0.516
7.907 ± 0.357
5.728 ± 0.303
5.868 ± 0.805
2.012 ± 0.168
2.934 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here