Sinomonas sp. R1AF57

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Sinomonas; unclassified Sinomonas

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3510 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221R506|A0A221R506_9MICC Amidohydrolase OS=Sinomonas sp. R1AF57 OX=2020377 GN=CGQ25_07745 PE=4 SV=1
MM1 pKa = 7.92LLGTLMAGGLFGLGQAAATAADD23 pKa = 3.99NTTLTTVPAVAATAPTQLTTDD44 pKa = 3.73GLLTGATGSGTGAGTGAVLGDD65 pKa = 3.66TTLTTVPAVAATAPTQLTTDD85 pKa = 3.57GLLTGTAGSGTGAGTGAVLGDD106 pKa = 3.65TALTTVPAVAATAPTQLTTDD126 pKa = 3.57GLLTGTAGSGTGAGTGAVLGDD147 pKa = 3.66TTLTTVPAVGATAPTQLTTGGLLTGSGTGAGTGAVLGDD185 pKa = 3.66TTLTTVPAVAATAPTQLTTDD205 pKa = 3.73GLLTGATGSGTGAGTGAVLGDD226 pKa = 3.66TTLTTVPAVCLSAPLSLTTNGEE248 pKa = 3.93AAALPAGTCGSIATPAAEE266 pKa = 4.98PGTGTPAGDD275 pKa = 3.66GAGSGTTPCSCTTGTAPGTVQDD297 pKa = 4.33AGAAAEE303 pKa = 4.44GVPGSATPAGVADD316 pKa = 4.06IPARR320 pKa = 11.84ATTGATAGSIAAAAPILTGAGGAYY344 pKa = 10.23AAGTASSGAAGPAVSTQGAGAAAASAAPSAAAAPAAAPAAMCLASMAEE392 pKa = 4.1DD393 pKa = 3.69TVGGVSLTLAALLAAAALAIGLTLGMVLGGTVRR426 pKa = 11.84RR427 pKa = 11.84RR428 pKa = 11.84VRR430 pKa = 11.84AA431 pKa = 3.41

Molecular weight: 38.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221R9D0|A0A221R9D0_9MICC Polyisoprenoid-binding protein OS=Sinomonas sp. R1AF57 OX=2020377 GN=CGQ25_13455 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3510

0

3510

1169589

32

2001

333.2

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.306 ± 0.07

0.596 ± 0.01

5.56 ± 0.031

5.814 ± 0.045

3.084 ± 0.025

9.572 ± 0.038

2.122 ± 0.018

3.773 ± 0.028

2.219 ± 0.027

10.174 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.015

1.92 ± 0.023

5.673 ± 0.035

2.832 ± 0.023

7.297 ± 0.042

5.511 ± 0.031

5.563 ± 0.035

8.689 ± 0.032

1.475 ± 0.016

2.008 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski