Garrulus glandarius associated circular virus 1
Average proteome isoelectric point is 7.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A218L3K4|A0A218L3K4_9CIRC Putative cap OS=Garrulus glandarius associated circular virus 1 OX=2006642 PE=4 SV=1
MM1 pKa = 7.02 ATSVPTVPGKK11 pKa = 10.32 LGKK14 pKa = 9.96 SRR16 pKa = 11.84 SCCWTLNNYY25 pKa = 9.78 HH26 pKa = 7.57 DD27 pKa = 5.34 DD28 pKa = 4.49 DD29 pKa = 5.54 IIRR32 pKa = 11.84 LRR34 pKa = 11.84 HH35 pKa = 5.28 YY36 pKa = 10.5 GKK38 pKa = 10.1 NSCRR42 pKa = 11.84 YY43 pKa = 7.88 MVFGYY48 pKa = 10.01 EE49 pKa = 4.12 VAPGTGTPHH58 pKa = 5.75 LQGYY62 pKa = 7.21 VSWEE66 pKa = 3.9 NPRR69 pKa = 11.84 SLDD72 pKa = 3.43 AFKK75 pKa = 11.42 LEE77 pKa = 3.87 ISQRR81 pKa = 11.84 LHH83 pKa = 6.67 LEE85 pKa = 4.06 KK86 pKa = 10.38 TRR88 pKa = 11.84 GTPKK92 pKa = 10.19 QASDD96 pKa = 3.54 YY97 pKa = 10.44 CKK99 pKa = 10.37 EE100 pKa = 4.0 DD101 pKa = 3.13 GKK103 pKa = 11.23 FEE105 pKa = 4.28 EE106 pKa = 5.08 FGEE109 pKa = 4.3 LPRR112 pKa = 11.84 QGEE115 pKa = 4.17 RR116 pKa = 11.84 TDD118 pKa = 2.87 WCVAISQIQSGIPVEE133 pKa = 4.29 EE134 pKa = 4.52 VIEE137 pKa = 4.09 NQPQLLPCIRR147 pKa = 11.84 ALDD150 pKa = 3.83 TFKK153 pKa = 11.05 ARR155 pKa = 11.84 QIKK158 pKa = 8.91 PLHH161 pKa = 6.39 RR162 pKa = 11.84 EE163 pKa = 3.72 VTVYY167 pKa = 10.32 VLWGDD172 pKa = 3.35 AGTGKK177 pKa = 10.37 SRR179 pKa = 11.84 WAYY182 pKa = 10.6 DD183 pKa = 3.63 NYY185 pKa = 10.53 PEE187 pKa = 5.67 LYY189 pKa = 10.24 SKK191 pKa = 10.83 PPGKK195 pKa = 9.27 WWDD198 pKa = 3.66 GYY200 pKa = 11.04 LGQKK204 pKa = 9.25 TILLDD209 pKa = 3.63 DD210 pKa = 4.56 FYY212 pKa = 11.57 GYY214 pKa = 10.1 IPYY217 pKa = 9.39 TEE219 pKa = 4.71 LLNVLDD225 pKa = 5.04 RR226 pKa = 11.84 YY227 pKa = 9.9 PYY229 pKa = 9.68 HH230 pKa = 7.95 AEE232 pKa = 3.92 VKK234 pKa = 10.37 NGYY237 pKa = 8.25 VWAQWDD243 pKa = 4.04 TVIITSNKK251 pKa = 9.1 PPNLWYY257 pKa = 10.38 KK258 pKa = 11.01 GIGLTPALQRR268 pKa = 11.84 RR269 pKa = 11.84 LNKK272 pKa = 9.82 ISFYY276 pKa = 11.02 GIDD279 pKa = 3.9 GKK281 pKa = 10.45 IEE283 pKa = 4.23 DD284 pKa = 4.48 ASCPPEE290 pKa = 4.18 AQSRR294 pKa = 11.84 SAA296 pKa = 4.19
Molecular weight: 33.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.66
IPC2_protein 6.81
IPC_protein 6.898
Toseland 6.707
ProMoST 7.38
Dawson 7.497
Bjellqvist 7.571
Wikipedia 7.439
Rodwell 7.497
Grimsley 6.766
Solomon 7.571
Lehninger 7.585
Nozaki 7.819
DTASelect 7.673
Thurlkill 7.702
EMBOSS 7.761
Sillero 7.951
Patrickios 3.846
IPC_peptide 7.556
IPC2_peptide 7.146
IPC2.peptide.svr19 7.172
Protein with the highest isoelectric point:
>tr|A0A218L3K4|A0A218L3K4_9CIRC Putative cap OS=Garrulus glandarius associated circular virus 1 OX=2006642 PE=4 SV=1
MM1 pKa = 7.66 AKK3 pKa = 10.16 SKK5 pKa = 9.17 MRR7 pKa = 11.84 RR8 pKa = 11.84 VPRR11 pKa = 11.84 KK12 pKa = 9.23 RR13 pKa = 11.84 RR14 pKa = 11.84 AGLRR18 pKa = 11.84 KK19 pKa = 9.4 RR20 pKa = 11.84 RR21 pKa = 11.84 VGKK24 pKa = 10.29 ARR26 pKa = 11.84 ASNVRR31 pKa = 11.84 DD32 pKa = 3.74 FASLSVSRR40 pKa = 11.84 TFTVPGSPPTQPIANVMYY58 pKa = 11.31 SMMATQLTQYY68 pKa = 9.11 TRR70 pKa = 11.84 AVQAAQAYY78 pKa = 8.29 QYY80 pKa = 11.24 YY81 pKa = 9.8 RR82 pKa = 11.84 IKK84 pKa = 10.62 RR85 pKa = 11.84 VSLKK89 pKa = 10.57 LKK91 pKa = 9.88 PQFDD95 pKa = 4.61 TYY97 pKa = 11.43 ASSANAYY104 pKa = 7.41 GKK106 pKa = 9.74 PKK108 pKa = 10.32 LYY110 pKa = 10.89 YY111 pKa = 10.08 IIDD114 pKa = 3.64 KK115 pKa = 11.05 SGALPTNVSLEE126 pKa = 4.12 SLKK129 pKa = 10.97 QMGARR134 pKa = 11.84 PRR136 pKa = 11.84 NLDD139 pKa = 3.45 EE140 pKa = 4.44 KK141 pKa = 11.02 ALTISFSPTVLNSAMTAGGGAFTTAPSQYY170 pKa = 10.74 QVTPWLNTNANTVAPGVWTANDD192 pKa = 3.43 VDD194 pKa = 4.41 HH195 pKa = 7.15 LGCYY199 pKa = 9.12 WYY201 pKa = 10.95 VEE203 pKa = 4.32 STSYY207 pKa = 11.21 GGTGGLPYY215 pKa = 10.44 QVDD218 pKa = 3.66 VEE220 pKa = 4.58 VQFEE224 pKa = 4.36 FKK226 pKa = 10.66 KK227 pKa = 10.36 PLWSALSSTTAIEE240 pKa = 4.15 LVPAILDD247 pKa = 3.7 ASPDD251 pKa = 3.77 GVEE254 pKa = 4.59 GGSDD258 pKa = 4.47 GITIPLIHH266 pKa = 6.93
Molecular weight: 29.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.857
IPC2_protein 9.472
IPC_protein 9.56
Toseland 9.853
ProMoST 9.663
Dawson 10.131
Bjellqvist 9.853
Wikipedia 10.35
Rodwell 10.423
Grimsley 10.233
Solomon 10.16
Lehninger 10.116
Nozaki 9.853
DTASelect 9.853
Thurlkill 9.955
EMBOSS 10.277
Sillero 10.043
Patrickios 5.436
IPC_peptide 10.145
IPC2_peptide 8.521
IPC2.peptide.svr19 8.491
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
562
266
296
281.0
31.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.651 ± 1.889
1.423 ± 0.688
4.804 ± 0.686
4.448 ± 1.193
2.491 ± 0.092
7.651 ± 0.334
1.423 ± 0.441
4.804 ± 0.686
6.05 ± 0.27
8.007 ± 0.321
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.601 ± 0.677
3.737 ± 0.015
6.94 ± 0.113
4.448 ± 0.041
5.872 ± 0.094
7.295 ± 1.135
6.762 ± 0.991
6.228 ± 0.848
2.491 ± 0.649
5.872 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here