Chloris striate mosaic virus (CSMV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P18921|REPA_CSMV Replication-associated protein A OS=Chloris striate mosaic virus OX=10820 GN=C1 PE=3 SV=1
MM1 pKa = 7.48SSLPVSEE8 pKa = 4.93SEE10 pKa = 4.53GEE12 pKa = 4.15GSGTSVQVPSRR23 pKa = 11.84GGQVTPGEE31 pKa = 4.43KK32 pKa = 10.33AFSLRR37 pKa = 11.84TKK39 pKa = 10.36HH40 pKa = 6.58VFLTYY45 pKa = 9.29PRR47 pKa = 11.84CPISPEE53 pKa = 3.63EE54 pKa = 4.19AGQKK58 pKa = 9.55IADD61 pKa = 3.89RR62 pKa = 11.84LKK64 pKa = 10.81NKK66 pKa = 8.98KK67 pKa = 9.79CNYY70 pKa = 9.36IYY72 pKa = 10.41ISRR75 pKa = 11.84EE76 pKa = 3.42FHH78 pKa = 7.42ADD80 pKa = 3.65GEE82 pKa = 4.57PHH84 pKa = 6.35LHH86 pKa = 6.94AFVQLEE92 pKa = 4.18ANFRR96 pKa = 11.84TTSPKK101 pKa = 10.57YY102 pKa = 9.07FDD104 pKa = 4.96LDD106 pKa = 3.54EE107 pKa = 4.3FHH109 pKa = 7.51PNIQAARR116 pKa = 11.84QPASTLKK123 pKa = 9.73YY124 pKa = 10.1CMKK127 pKa = 10.23HH128 pKa = 6.39PEE130 pKa = 4.17SSWEE134 pKa = 3.67FGKK137 pKa = 10.47FLKK140 pKa = 10.6PKK142 pKa = 9.64VNRR145 pKa = 11.84SPTQSASRR153 pKa = 11.84DD154 pKa = 3.37KK155 pKa = 11.08TMKK158 pKa = 10.43QIMANATSRR167 pKa = 11.84DD168 pKa = 3.71EE169 pKa = 4.09YY170 pKa = 11.18LSMVRR175 pKa = 11.84KK176 pKa = 10.02SFPFEE181 pKa = 3.44WAVRR185 pKa = 11.84LQQFQYY191 pKa = 10.6SANALFPDD199 pKa = 4.65PPQTYY204 pKa = 8.8SAPYY208 pKa = 9.77ASRR211 pKa = 11.84DD212 pKa = 3.67MSDD215 pKa = 3.35HH216 pKa = 6.14PVIGEE221 pKa = 3.86WLQQEE226 pKa = 5.58LYY228 pKa = 9.55TVSPQALSLHH238 pKa = 6.3AGISEE243 pKa = 4.0EE244 pKa = 3.98QARR247 pKa = 11.84IDD249 pKa = 4.02LQWMSDD255 pKa = 3.57LTRR258 pKa = 11.84SGALEE263 pKa = 4.49SGDD266 pKa = 3.86EE267 pKa = 4.29ACTSVGQQEE276 pKa = 4.49LEE278 pKa = 3.94RR279 pKa = 11.84LLGPEE284 pKa = 4.04VLEE287 pKa = 5.53LITTGSTQQ295 pKa = 2.91

Molecular weight: 33.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P18919|REP_CSMV Replication-associated protein OS=Chloris striate mosaic virus OX=10820 GN=C1/C2 PE=3 SV=2
MM1 pKa = 7.48SPASSWKK8 pKa = 9.62RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84PSSSSAQASKK21 pKa = 10.56KK22 pKa = 9.43RR23 pKa = 11.84RR24 pKa = 11.84VYY26 pKa = 10.13RR27 pKa = 11.84PAVSRR32 pKa = 11.84SLARR36 pKa = 11.84RR37 pKa = 11.84EE38 pKa = 3.83PLQVQDD44 pKa = 5.05FVWDD48 pKa = 3.35TDD50 pKa = 3.64VAFNRR55 pKa = 11.84GGGCYY60 pKa = 10.38LLTSYY65 pKa = 11.4ARR67 pKa = 11.84GSAEE71 pKa = 4.04NQRR74 pKa = 11.84KK75 pKa = 5.73TAEE78 pKa = 4.12TITYY82 pKa = 9.28KK83 pKa = 10.76VAVNLGCAISGTMQQYY99 pKa = 10.37CISSRR104 pKa = 11.84PVCWIVYY111 pKa = 9.2DD112 pKa = 4.12AAPTGSAVTPKK123 pKa = 10.6DD124 pKa = 2.86IFGYY128 pKa = 10.02PEE130 pKa = 4.68GLVNWPTTWKK140 pKa = 9.23VARR143 pKa = 11.84AVSHH147 pKa = 6.15RR148 pKa = 11.84FIVKK152 pKa = 9.7RR153 pKa = 11.84RR154 pKa = 11.84WVFTMEE160 pKa = 4.52SNGSRR165 pKa = 11.84FDD167 pKa = 3.73RR168 pKa = 11.84DD169 pKa = 3.8YY170 pKa = 11.11TNLPAAIPQSLPVLNKK186 pKa = 9.32FAKK189 pKa = 9.26QLGVRR194 pKa = 11.84TEE196 pKa = 3.78WKK198 pKa = 9.77NAEE201 pKa = 4.27GGDD204 pKa = 3.68FGDD207 pKa = 3.81IKK209 pKa = 11.1SGALYY214 pKa = 10.76LVMAPANGAVFVARR228 pKa = 11.84GNVRR232 pKa = 11.84VYY234 pKa = 10.13FKK236 pKa = 11.12SVGNQQ241 pKa = 2.8

Molecular weight: 26.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

897

241

361

299.0

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.469 ± 1.082

1.672 ± 0.143

4.013 ± 0.098

5.909 ± 1.218

4.682 ± 0.268

6.02 ± 0.514

2.341 ± 0.68

3.79 ± 0.291

5.686 ± 0.257

6.577 ± 0.746

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.007 ± 0.17

3.567 ± 0.536

6.912 ± 0.542

5.574 ± 0.713

6.243 ± 0.867

9.699 ± 0.484

5.463 ± 0.128

6.132 ± 1.242

2.341 ± 0.418

3.902 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski