Microbacterium phage Kieran

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4A2L0|A0A2R4A2L0_9CAUD Uncharacterized protein OS=Microbacterium phage Kieran OX=2126931 GN=21 PE=4 SV=1
MM1 pKa = 7.84TDD3 pKa = 3.7FQTALDD9 pKa = 3.67EE10 pKa = 4.42HH11 pKa = 7.03LPLRR15 pKa = 11.84TAGALPGTRR24 pKa = 11.84TFGQVEE30 pKa = 4.16EE31 pKa = 4.82ALNSADD37 pKa = 3.19IDD39 pKa = 3.91EE40 pKa = 4.74VKK42 pKa = 11.01AEE44 pKa = 4.07LVHH47 pKa = 6.66TRR49 pKa = 11.84AHH51 pKa = 5.2YY52 pKa = 9.77QALEE56 pKa = 3.81YY57 pKa = 9.93MLNSEE62 pKa = 4.19EE63 pKa = 5.13RR64 pKa = 11.84ILEE67 pKa = 4.41TILAGIARR75 pKa = 11.84DD76 pKa = 3.64AEE78 pKa = 4.32LGEE81 pKa = 4.34EE82 pKa = 4.5AEE84 pKa = 4.63VPAPQTVEE92 pKa = 3.64EE93 pKa = 4.12AVRR96 pKa = 11.84LVVRR100 pKa = 11.84LYY102 pKa = 10.87GSVIVEE108 pKa = 4.15SAYY111 pKa = 9.84DD112 pKa = 3.72TAHH115 pKa = 5.29STGFFMGVLGMDD127 pKa = 4.57LQTSLQNLFGDD138 pKa = 5.06LDD140 pKa = 3.46WSDD143 pKa = 4.12PEE145 pKa = 4.77RR146 pKa = 11.84EE147 pKa = 3.93LSEE150 pKa = 6.15AEE152 pKa = 4.17LQALATPVVDD162 pKa = 4.64TEE164 pKa = 4.38DD165 pKa = 3.1

Molecular weight: 18.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4A2F3|A0A2R4A2F3_9CAUD Cas4 family exonuclease OS=Microbacterium phage Kieran OX=2126931 GN=26 PE=4 SV=1
MM1 pKa = 6.94TRR3 pKa = 11.84SHH5 pKa = 6.86AKK7 pKa = 10.08SIARR11 pKa = 11.84SAEE14 pKa = 3.99LTSLAGRR21 pKa = 11.84RR22 pKa = 11.84WGPKK26 pKa = 8.8RR27 pKa = 11.84RR28 pKa = 11.84WRR30 pKa = 11.84CVYY33 pKa = 9.89CGRR36 pKa = 11.84GAGMVVDD43 pKa = 4.01HH44 pKa = 6.6FVPEE48 pKa = 4.13KK49 pKa = 10.63RR50 pKa = 11.84GGEE53 pKa = 3.76DD54 pKa = 3.11TVFNLVPACDD64 pKa = 3.59RR65 pKa = 11.84CNSSKK70 pKa = 10.39QDD72 pKa = 3.71HH73 pKa = 6.37EE74 pKa = 4.4PEE76 pKa = 3.63AWMQAVGVPEE86 pKa = 4.04ARR88 pKa = 11.84RR89 pKa = 11.84AALWRR94 pKa = 11.84ILHH97 pKa = 6.22LPSSASLTVPAEE109 pKa = 4.0RR110 pKa = 11.84FEE112 pKa = 4.95LDD114 pKa = 3.11YY115 pKa = 11.42GAAKK119 pKa = 10.41ALRR122 pKa = 11.84RR123 pKa = 11.84PFQGAKK129 pKa = 10.23GGG131 pKa = 3.68

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13011

39

876

203.3

22.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.805 ± 0.356

0.4 ± 0.074

6.118 ± 0.288

6.218 ± 0.258

3.22 ± 0.167

8.885 ± 0.366

1.745 ± 0.17

3.72 ± 0.205

4.135 ± 0.261

8.877 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.131

2.959 ± 0.196

5.211 ± 0.212

3.597 ± 0.175

6.387 ± 0.355

5.465 ± 0.275

6.249 ± 0.287

8.477 ± 0.287

2.098 ± 0.155

2.444 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski