Amino acid dipepetide frequency for Escherichia phage Minorna

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.271AlaAla: 14.271 ± 1.248
0.732AlaCys: 0.732 ± 0.268
5.855AlaAsp: 5.855 ± 0.737
5.489AlaGlu: 5.489 ± 0.624
2.781AlaPhe: 2.781 ± 0.401
8.636AlaGly: 8.636 ± 1.154
1.756AlaHis: 1.756 ± 0.438
5.489AlaIle: 5.489 ± 0.65
4.757AlaLys: 4.757 ± 0.877
9.294AlaLeu: 9.294 ± 0.86
3.001AlaMet: 3.001 ± 0.397
2.708AlaAsn: 2.708 ± 0.415
4.537AlaPro: 4.537 ± 1.185
4.977AlaGln: 4.977 ± 0.82
5.343AlaArg: 5.343 ± 0.691
6.001AlaSer: 6.001 ± 0.795
5.05AlaThr: 5.05 ± 0.64
7.611AlaVal: 7.611 ± 0.894
1.244AlaTrp: 1.244 ± 0.356
3.952AlaTyr: 3.952 ± 0.508
0.0AlaXaa: 0.0 ± 0.0
Cys
0.805CysAla: 0.805 ± 0.297
0.293CysCys: 0.293 ± 0.17
0.951CysAsp: 0.951 ± 0.289
0.366CysGlu: 0.366 ± 0.167
0.366CysPhe: 0.366 ± 0.184
0.878CysGly: 0.878 ± 0.289
0.366CysHis: 0.366 ± 0.175
0.585CysIle: 0.585 ± 0.293
0.439CysLys: 0.439 ± 0.252
0.951CysLeu: 0.951 ± 0.344
0.585CysMet: 0.585 ± 0.238
0.366CysAsn: 0.366 ± 0.175
0.732CysPro: 0.732 ± 0.258
0.366CysGln: 0.366 ± 0.147
1.025CysArg: 1.025 ± 0.28
0.732CysSer: 0.732 ± 0.241
0.878CysThr: 0.878 ± 0.227
0.878CysVal: 0.878 ± 0.295
0.293CysTrp: 0.293 ± 0.151
0.732CysTyr: 0.732 ± 0.237
0.0CysXaa: 0.0 ± 0.0
Asp
7.465AspAla: 7.465 ± 0.993
1.391AspCys: 1.391 ± 0.461
3.074AspAsp: 3.074 ± 0.547
3.074AspGlu: 3.074 ± 0.461
2.415AspPhe: 2.415 ± 0.339
5.343AspGly: 5.343 ± 0.719
0.585AspHis: 0.585 ± 0.222
3.586AspIle: 3.586 ± 0.533
2.635AspLys: 2.635 ± 0.546
5.855AspLeu: 5.855 ± 0.461
2.342AspMet: 2.342 ± 0.424
2.635AspAsn: 2.635 ± 0.383
2.049AspPro: 2.049 ± 0.472
1.464AspGln: 1.464 ± 0.252
2.561AspArg: 2.561 ± 0.506
4.684AspSer: 4.684 ± 0.486
4.025AspThr: 4.025 ± 0.707
4.172AspVal: 4.172 ± 0.461
0.878AspTrp: 0.878 ± 0.214
2.781AspTyr: 2.781 ± 0.508
0.0AspXaa: 0.0 ± 0.0
Glu
5.196GluAla: 5.196 ± 0.767
0.512GluCys: 0.512 ± 0.215
3.22GluAsp: 3.22 ± 0.47
3.367GluGlu: 3.367 ± 0.861
2.269GluPhe: 2.269 ± 0.435
3.147GluGly: 3.147 ± 0.35
2.269GluHis: 2.269 ± 0.466
1.976GluIle: 1.976 ± 0.372
1.903GluLys: 1.903 ± 0.394
5.782GluLeu: 5.782 ± 0.654
2.269GluMet: 2.269 ± 0.366
1.61GluAsn: 1.61 ± 0.396
1.976GluPro: 1.976 ± 0.467
3.22GluGln: 3.22 ± 0.517
3.367GluArg: 3.367 ± 0.56
3.22GluSer: 3.22 ± 0.462
2.708GluThr: 2.708 ± 0.452
4.684GluVal: 4.684 ± 0.6
0.878GluTrp: 0.878 ± 0.196
2.635GluTyr: 2.635 ± 0.401
0.0GluXaa: 0.0 ± 0.0
Phe
2.927PheAla: 2.927 ± 0.386
0.512PheCys: 0.512 ± 0.227
2.049PheAsp: 2.049 ± 0.376
1.903PheGlu: 1.903 ± 0.324
1.025PhePhe: 1.025 ± 0.25
1.976PheGly: 1.976 ± 0.36
0.512PheHis: 0.512 ± 0.197
1.464PheIle: 1.464 ± 0.342
2.415PheLys: 2.415 ± 0.454
2.196PheLeu: 2.196 ± 0.442
0.512PheMet: 0.512 ± 0.219
1.683PheAsn: 1.683 ± 0.373
1.537PhePro: 1.537 ± 0.271
1.171PheGln: 1.171 ± 0.215
1.391PheArg: 1.391 ± 0.363
1.537PheSer: 1.537 ± 0.329
1.683PheThr: 1.683 ± 0.317
1.83PheVal: 1.83 ± 0.401
0.585PheTrp: 0.585 ± 0.193
1.244PheTyr: 1.244 ± 0.313
0.0PheXaa: 0.0 ± 0.0
Gly
5.855GlyAla: 5.855 ± 0.763
1.317GlyCys: 1.317 ± 0.354
4.684GlyAsp: 4.684 ± 0.63
3.732GlyGlu: 3.732 ± 0.537
2.635GlyPhe: 2.635 ± 0.486
5.196GlyGly: 5.196 ± 0.707
0.951GlyHis: 0.951 ± 0.283
4.684GlyIle: 4.684 ± 0.498
3.879GlyLys: 3.879 ± 0.55
5.855GlyLeu: 5.855 ± 0.641
1.903GlyMet: 1.903 ± 0.397
3.001GlyAsn: 3.001 ± 0.472
1.756GlyPro: 1.756 ± 0.337
3.147GlyGln: 3.147 ± 0.545
5.416GlyArg: 5.416 ± 0.595
5.416GlySer: 5.416 ± 0.779
5.562GlyThr: 5.562 ± 0.85
5.635GlyVal: 5.635 ± 0.621
0.659GlyTrp: 0.659 ± 0.244
3.732GlyTyr: 3.732 ± 0.766
0.0GlyXaa: 0.0 ± 0.0
His
1.683HisAla: 1.683 ± 0.394
0.366HisCys: 0.366 ± 0.256
1.464HisAsp: 1.464 ± 0.4
1.391HisGlu: 1.391 ± 0.368
0.22HisPhe: 0.22 ± 0.108
2.122HisGly: 2.122 ± 0.468
0.073HisHis: 0.073 ± 0.066
0.805HisIle: 0.805 ± 0.291
0.732HisLys: 0.732 ± 0.216
2.781HisLeu: 2.781 ± 0.622
0.366HisMet: 0.366 ± 0.126
0.805HisAsn: 0.805 ± 0.281
0.585HisPro: 0.585 ± 0.271
0.878HisGln: 0.878 ± 0.272
1.317HisArg: 1.317 ± 0.342
0.805HisSer: 0.805 ± 0.261
0.878HisThr: 0.878 ± 0.227
1.098HisVal: 1.098 ± 0.282
0.22HisTrp: 0.22 ± 0.121
0.805HisTyr: 0.805 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
3.44IleAla: 3.44 ± 0.559
0.512IleCys: 0.512 ± 0.185
2.635IleAsp: 2.635 ± 0.302
2.488IleGlu: 2.488 ± 0.569
0.659IlePhe: 0.659 ± 0.209
2.927IleGly: 2.927 ± 0.518
0.805IleHis: 0.805 ± 0.231
2.122IleIle: 2.122 ± 0.367
3.147IleLys: 3.147 ± 0.512
4.757IleLeu: 4.757 ± 0.621
1.098IleMet: 1.098 ± 0.228
2.488IleAsn: 2.488 ± 0.468
2.854IlePro: 2.854 ± 0.508
2.781IleGln: 2.781 ± 0.575
2.927IleArg: 2.927 ± 0.416
4.025IleSer: 4.025 ± 0.499
2.781IleThr: 2.781 ± 0.602
2.781IleVal: 2.781 ± 0.419
0.22IleTrp: 0.22 ± 0.137
1.61IleTyr: 1.61 ± 0.369
0.0IleXaa: 0.0 ± 0.0
Lys
6.44LysAla: 6.44 ± 0.884
0.732LysCys: 0.732 ± 0.229
2.342LysAsp: 2.342 ± 0.305
3.732LysGlu: 3.732 ± 0.478
1.098LysPhe: 1.098 ± 0.284
3.001LysGly: 3.001 ± 0.487
1.025LysHis: 1.025 ± 0.257
1.683LysIle: 1.683 ± 0.335
1.976LysLys: 1.976 ± 0.396
4.83LysLeu: 4.83 ± 0.575
1.244LysMet: 1.244 ± 0.279
1.244LysAsn: 1.244 ± 0.26
1.976LysPro: 1.976 ± 0.493
3.22LysGln: 3.22 ± 0.489
3.147LysArg: 3.147 ± 0.544
2.635LysSer: 2.635 ± 0.418
2.708LysThr: 2.708 ± 0.421
3.293LysVal: 3.293 ± 0.432
0.878LysTrp: 0.878 ± 0.281
1.83LysTyr: 1.83 ± 0.456
0.0LysXaa: 0.0 ± 0.0
Leu
8.27LeuAla: 8.27 ± 0.881
1.244LeuCys: 1.244 ± 0.405
7.465LeuAsp: 7.465 ± 0.633
5.855LeuGlu: 5.855 ± 0.555
2.488LeuPhe: 2.488 ± 0.393
6.879LeuGly: 6.879 ± 0.74
1.756LeuHis: 1.756 ± 0.39
4.172LeuIle: 4.172 ± 0.613
4.025LeuLys: 4.025 ± 0.473
6.953LeuLeu: 6.953 ± 0.714
1.83LeuMet: 1.83 ± 0.302
3.367LeuAsn: 3.367 ± 0.419
3.44LeuPro: 3.44 ± 0.514
4.098LeuGln: 4.098 ± 0.567
6.001LeuArg: 6.001 ± 0.683
5.562LeuSer: 5.562 ± 0.634
5.489LeuThr: 5.489 ± 0.647
5.855LeuVal: 5.855 ± 0.767
1.098LeuTrp: 1.098 ± 0.271
3.732LeuTyr: 3.732 ± 0.574
0.0LeuXaa: 0.0 ± 0.0
Met
2.708MetAla: 2.708 ± 0.412
0.22MetCys: 0.22 ± 0.107
2.415MetAsp: 2.415 ± 0.467
0.951MetGlu: 0.951 ± 0.208
1.098MetPhe: 1.098 ± 0.291
1.317MetGly: 1.317 ± 0.254
0.512MetHis: 0.512 ± 0.216
0.951MetIle: 0.951 ± 0.309
1.098MetLys: 1.098 ± 0.302
3.806MetLeu: 3.806 ± 0.46
0.659MetMet: 0.659 ± 0.245
1.098MetAsn: 1.098 ± 0.318
1.244MetPro: 1.244 ± 0.232
1.903MetGln: 1.903 ± 0.354
1.756MetArg: 1.756 ± 0.437
2.122MetSer: 2.122 ± 0.479
0.951MetThr: 0.951 ± 0.312
1.83MetVal: 1.83 ± 0.37
0.512MetTrp: 0.512 ± 0.179
1.244MetTyr: 1.244 ± 0.266
0.0MetXaa: 0.0 ± 0.0
Asn
3.367AsnAla: 3.367 ± 0.477
0.146AsnCys: 0.146 ± 0.131
1.83AsnAsp: 1.83 ± 0.424
1.756AsnGlu: 1.756 ± 0.356
1.683AsnPhe: 1.683 ± 0.387
3.44AsnGly: 3.44 ± 0.545
0.293AsnHis: 0.293 ± 0.128
2.269AsnIle: 2.269 ± 0.43
2.415AsnLys: 2.415 ± 0.395
3.659AsnLeu: 3.659 ± 0.664
0.951AsnMet: 0.951 ± 0.259
2.122AsnAsn: 2.122 ± 0.517
2.415AsnPro: 2.415 ± 0.531
1.61AsnGln: 1.61 ± 0.333
1.683AsnArg: 1.683 ± 0.32
2.781AsnSer: 2.781 ± 0.574
3.001AsnThr: 3.001 ± 0.551
3.513AsnVal: 3.513 ± 0.702
0.585AsnTrp: 0.585 ± 0.202
1.537AsnTyr: 1.537 ± 0.358
0.0AsnXaa: 0.0 ± 0.0
Pro
4.318ProAla: 4.318 ± 0.629
0.293ProCys: 0.293 ± 0.131
2.635ProAsp: 2.635 ± 0.469
3.293ProGlu: 3.293 ± 0.406
1.244ProPhe: 1.244 ± 0.307
2.488ProGly: 2.488 ± 0.482
0.585ProHis: 0.585 ± 0.221
1.391ProIle: 1.391 ± 0.313
1.683ProLys: 1.683 ± 0.355
3.147ProLeu: 3.147 ± 0.575
1.098ProMet: 1.098 ± 0.22
2.196ProAsn: 2.196 ± 0.46
0.732ProPro: 0.732 ± 0.248
1.317ProGln: 1.317 ± 0.29
1.976ProArg: 1.976 ± 0.395
2.122ProSer: 2.122 ± 0.442
2.927ProThr: 2.927 ± 0.505
3.001ProVal: 3.001 ± 0.486
0.512ProTrp: 0.512 ± 0.195
1.391ProTyr: 1.391 ± 0.331
0.0ProXaa: 0.0 ± 0.0
Gln
5.196GlnAla: 5.196 ± 0.752
0.293GlnCys: 0.293 ± 0.181
3.293GlnAsp: 3.293 ± 0.573
3.22GlnGlu: 3.22 ± 0.513
1.171GlnPhe: 1.171 ± 0.288
3.293GlnGly: 3.293 ± 0.508
1.171GlnHis: 1.171 ± 0.399
1.317GlnIle: 1.317 ± 0.3
2.122GlnLys: 2.122 ± 0.423
4.977GlnLeu: 4.977 ± 0.523
1.171GlnMet: 1.171 ± 0.281
2.049GlnAsn: 2.049 ± 0.347
1.391GlnPro: 1.391 ± 0.437
3.001GlnGln: 3.001 ± 0.652
3.147GlnArg: 3.147 ± 0.426
2.854GlnSer: 2.854 ± 0.386
1.391GlnThr: 1.391 ± 0.428
2.927GlnVal: 2.927 ± 0.528
0.512GlnTrp: 0.512 ± 0.184
2.049GlnTyr: 2.049 ± 0.356
0.0GlnXaa: 0.0 ± 0.0
Arg
6.44ArgAla: 6.44 ± 0.916
0.585ArgCys: 0.585 ± 0.241
2.708ArgAsp: 2.708 ± 0.403
3.586ArgGlu: 3.586 ± 0.513
2.269ArgPhe: 2.269 ± 0.37
3.952ArgGly: 3.952 ± 0.663
1.171ArgHis: 1.171 ± 0.247
2.854ArgIle: 2.854 ± 0.58
3.586ArgLys: 3.586 ± 0.538
4.537ArgLeu: 4.537 ± 0.462
2.269ArgMet: 2.269 ± 0.377
2.708ArgAsn: 2.708 ± 0.39
1.171ArgPro: 1.171 ± 0.307
2.415ArgGln: 2.415 ± 0.437
3.659ArgArg: 3.659 ± 0.675
3.513ArgSer: 3.513 ± 0.667
3.147ArgThr: 3.147 ± 0.389
3.659ArgVal: 3.659 ± 0.489
0.805ArgTrp: 0.805 ± 0.237
1.61ArgTyr: 1.61 ± 0.313
0.0ArgXaa: 0.0 ± 0.0
Ser
7.904SerAla: 7.904 ± 0.918
0.732SerCys: 0.732 ± 0.289
4.098SerAsp: 4.098 ± 0.459
2.708SerGlu: 2.708 ± 0.519
1.61SerPhe: 1.61 ± 0.33
6.074SerGly: 6.074 ± 0.848
0.878SerHis: 0.878 ± 0.225
3.001SerIle: 3.001 ± 0.749
4.172SerLys: 4.172 ± 0.641
4.245SerLeu: 4.245 ± 0.517
2.049SerMet: 2.049 ± 0.401
2.415SerAsn: 2.415 ± 0.433
2.708SerPro: 2.708 ± 0.337
2.269SerGln: 2.269 ± 0.462
2.561SerArg: 2.561 ± 0.511
3.367SerSer: 3.367 ± 0.663
3.806SerThr: 3.806 ± 0.479
4.172SerVal: 4.172 ± 0.479
1.903SerTrp: 1.903 ± 0.47
1.537SerTyr: 1.537 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
6.148ThrAla: 6.148 ± 1.064
0.732ThrCys: 0.732 ± 0.312
3.732ThrAsp: 3.732 ± 0.417
2.561ThrGlu: 2.561 ± 0.497
1.903ThrPhe: 1.903 ± 0.403
5.05ThrGly: 5.05 ± 0.505
1.317ThrHis: 1.317 ± 0.444
2.927ThrIle: 2.927 ± 0.458
2.561ThrLys: 2.561 ± 0.505
4.83ThrLeu: 4.83 ± 0.762
1.61ThrMet: 1.61 ± 0.307
2.269ThrAsn: 2.269 ± 0.532
2.342ThrPro: 2.342 ± 0.344
2.269ThrGln: 2.269 ± 0.351
2.342ThrArg: 2.342 ± 0.426
4.245ThrSer: 4.245 ± 0.557
3.44ThrThr: 3.44 ± 0.636
4.464ThrVal: 4.464 ± 0.71
1.025ThrTrp: 1.025 ± 0.253
2.196ThrTyr: 2.196 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
7.026ValAla: 7.026 ± 0.766
0.805ValCys: 0.805 ± 0.28
5.196ValAsp: 5.196 ± 0.638
4.025ValGlu: 4.025 ± 0.602
1.171ValPhe: 1.171 ± 0.259
5.708ValGly: 5.708 ± 0.596
2.342ValHis: 2.342 ± 0.518
2.635ValIle: 2.635 ± 0.514
2.635ValLys: 2.635 ± 0.439
6.148ValLeu: 6.148 ± 0.67
2.196ValMet: 2.196 ± 0.357
3.074ValAsn: 3.074 ± 0.684
3.293ValPro: 3.293 ± 0.55
3.879ValGln: 3.879 ± 0.605
3.952ValArg: 3.952 ± 0.479
3.44ValSer: 3.44 ± 0.624
3.732ValThr: 3.732 ± 0.567
5.416ValVal: 5.416 ± 0.425
0.659ValTrp: 0.659 ± 0.243
2.488ValTyr: 2.488 ± 0.346
0.0ValXaa: 0.0 ± 0.0
Trp
1.025TrpAla: 1.025 ± 0.264
0.439TrpCys: 0.439 ± 0.171
0.951TrpAsp: 0.951 ± 0.241
1.098TrpGlu: 1.098 ± 0.328
0.805TrpPhe: 0.805 ± 0.298
0.659TrpGly: 0.659 ± 0.235
0.366TrpHis: 0.366 ± 0.152
0.585TrpIle: 0.585 ± 0.24
0.585TrpLys: 0.585 ± 0.267
1.391TrpLeu: 1.391 ± 0.299
0.293TrpMet: 0.293 ± 0.126
0.805TrpAsn: 0.805 ± 0.221
0.439TrpPro: 0.439 ± 0.196
0.512TrpGln: 0.512 ± 0.207
0.805TrpArg: 0.805 ± 0.238
0.585TrpSer: 0.585 ± 0.241
1.244TrpThr: 1.244 ± 0.253
1.025TrpVal: 1.025 ± 0.278
0.366TrpTrp: 0.366 ± 0.183
0.659TrpTyr: 0.659 ± 0.222
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.074TyrAla: 3.074 ± 0.512
0.732TyrCys: 0.732 ± 0.257
2.415TyrAsp: 2.415 ± 0.486
1.61TyrGlu: 1.61 ± 0.448
1.244TyrPhe: 1.244 ± 0.28
2.927TyrGly: 2.927 ± 0.52
0.659TyrHis: 0.659 ± 0.203
2.561TyrIle: 2.561 ± 0.496
2.269TyrLys: 2.269 ± 0.417
3.586TyrLeu: 3.586 ± 0.541
0.878TyrMet: 0.878 ± 0.264
2.415TyrAsn: 2.415 ± 0.482
1.171TyrPro: 1.171 ± 0.24
2.049TyrGln: 2.049 ± 0.418
2.269TyrArg: 2.269 ± 0.467
2.561TyrSer: 2.561 ± 0.338
2.561TyrThr: 2.561 ± 0.515
1.976TyrVal: 1.976 ± 0.483
0.805TyrTrp: 0.805 ± 0.267
1.098TyrTyr: 1.098 ± 0.279
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (13665 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski