Microbacterium phage BubbaBear

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T5L7|A0A4D6T5L7_9CAUD Minor tail protein OS=Microbacterium phage BubbaBear OX=2572529 GN=17 PE=4 SV=1
MM1 pKa = 7.79ADD3 pKa = 3.6EE4 pKa = 4.77EE5 pKa = 4.57LTPILWDD12 pKa = 3.36ATCRR16 pKa = 11.84TDD18 pKa = 2.9GCEE21 pKa = 3.8NADD24 pKa = 3.07ITFRR28 pKa = 11.84VPAHH32 pKa = 7.21PEE34 pKa = 3.59TPMILCGPCGTLITDD49 pKa = 4.4LTPSPEE55 pKa = 3.84GDD57 pKa = 3.01AA58 pKa = 4.77

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T6Z8|A0A4D6T6Z8_9CAUD Uncharacterized protein OS=Microbacterium phage BubbaBear OX=2572529 GN=42 PE=4 SV=1
MM1 pKa = 6.71THH3 pKa = 6.21PVVYY7 pKa = 9.97LVEE10 pKa = 4.58GADD13 pKa = 3.96GTGKK17 pKa = 9.02TSFVKK22 pKa = 10.79SMVSIHH28 pKa = 6.22AQLGDD33 pKa = 3.42RR34 pKa = 11.84EE35 pKa = 4.23PRR37 pKa = 11.84VIHH40 pKa = 6.8NDD42 pKa = 3.2ASDD45 pKa = 3.41HH46 pKa = 6.12KK47 pKa = 11.07LPGSLYY53 pKa = 8.85RR54 pKa = 11.84HH55 pKa = 5.92YY56 pKa = 10.58RR57 pKa = 11.84AQLLDD62 pKa = 5.06AIDD65 pKa = 3.99FRR67 pKa = 11.84DD68 pKa = 3.37NHH70 pKa = 6.7GISTYY75 pKa = 9.84IDD77 pKa = 3.14RR78 pKa = 11.84SFLSEE83 pKa = 4.2LVYY86 pKa = 11.11GPLYY90 pKa = 9.99RR91 pKa = 11.84SKK93 pKa = 11.16SRR95 pKa = 11.84ITRR98 pKa = 11.84RR99 pKa = 11.84QARR102 pKa = 11.84RR103 pKa = 11.84LEE105 pKa = 4.17RR106 pKa = 11.84LADD109 pKa = 3.48RR110 pKa = 11.84HH111 pKa = 6.22GIVLLGMTADD121 pKa = 3.59LNLRR125 pKa = 11.84RR126 pKa = 11.84LRR128 pKa = 11.84IRR130 pKa = 11.84ARR132 pKa = 11.84GEE134 pKa = 3.35TWTRR138 pKa = 11.84KK139 pKa = 9.61DD140 pKa = 3.3AFVGALYY147 pKa = 10.12SQHH150 pKa = 5.03FRR152 pKa = 11.84EE153 pKa = 4.63RR154 pKa = 11.84GRR156 pKa = 11.84YY157 pKa = 7.86WLTADD162 pKa = 3.59SSSATVLNN170 pKa = 4.16

Molecular weight: 19.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13168

42

920

193.6

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.703 ± 0.327

0.463 ± 0.11

6.607 ± 0.333

5.984 ± 0.277

3.212 ± 0.17

8.095 ± 0.328

1.876 ± 0.188

4.655 ± 0.225

4.215 ± 0.271

8.635 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.135

3.395 ± 0.227

5.308 ± 0.212

3.311 ± 0.153

6.372 ± 0.356

5.331 ± 0.278

6.797 ± 0.453

7.268 ± 0.308

2.119 ± 0.14

2.461 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski