Escherichia phage vB_EcoS-2004IV

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Braunvirinae; Veterinaerplatzvirus; unclassified Veterinaerplatzvirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P1M3R8|A0A5P1M3R8_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS-2004IV OX=2576488 GN=AOGNBOCH_00025 PE=4 SV=1
MM1 pKa = 6.98ATIYY5 pKa = 8.92TGKK8 pKa = 9.49MYY10 pKa = 10.47EE11 pKa = 4.12SRR13 pKa = 11.84KK14 pKa = 8.17TGYY17 pKa = 10.07EE18 pKa = 3.89YY19 pKa = 11.09EE20 pKa = 4.22LVITADD26 pKa = 4.31GYY28 pKa = 11.83VLDD31 pKa = 5.88DD32 pKa = 3.87GDD34 pKa = 4.32GYY36 pKa = 11.51AFEE39 pKa = 4.81VSPTYY44 pKa = 10.73KK45 pKa = 10.51DD46 pKa = 3.65DD47 pKa = 4.74LEE49 pKa = 4.56TVKK52 pKa = 10.87NAFGEE57 pKa = 4.31KK58 pKa = 9.32FDD60 pKa = 4.45EE61 pKa = 4.48VSEE64 pKa = 4.18

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P1M2Z3|A0A5P1M2Z3_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS-2004IV OX=2576488 GN=AOGNBOCH_00008 PE=4 SV=1
MM1 pKa = 7.18NAVDD5 pKa = 4.57KK6 pKa = 10.67RR7 pKa = 11.84TINGNNGTIRR17 pKa = 11.84TEE19 pKa = 4.03DD20 pKa = 2.82KK21 pKa = 10.5KK22 pKa = 10.72QRR24 pKa = 11.84KK25 pKa = 8.53RR26 pKa = 11.84PSGYY30 pKa = 10.06YY31 pKa = 8.95VLKK34 pKa = 10.9DD35 pKa = 3.51EE36 pKa = 4.52VRR38 pKa = 11.84AGLRR42 pKa = 11.84ARR44 pKa = 11.84LEE46 pKa = 3.84IVLDD50 pKa = 3.88FFGTKK55 pKa = 10.5ANMAKK60 pKa = 9.47QLKK63 pKa = 7.74VTPQAVEE70 pKa = 3.72EE71 pKa = 4.25WFKK74 pKa = 11.3RR75 pKa = 11.84GMMSARR81 pKa = 11.84GAQLSHH87 pKa = 6.72NYY89 pKa = 9.66YY90 pKa = 10.0KK91 pKa = 10.9RR92 pKa = 11.84NGEE95 pKa = 3.9GFRR98 pKa = 11.84ATFCRR103 pKa = 11.84PDD105 pKa = 3.38LQFDD109 pKa = 4.36GNGKK113 pKa = 8.92PLTLRR118 pKa = 11.84CKK120 pKa = 9.49KK121 pKa = 9.98RR122 pKa = 11.84HH123 pKa = 4.86MLRR126 pKa = 11.84VVTEE130 pKa = 4.33AEE132 pKa = 4.14LATKK136 pKa = 9.16PEE138 pKa = 4.04CRR140 pKa = 11.84SWRR143 pKa = 11.84KK144 pKa = 9.4IKK146 pKa = 10.36AANEE150 pKa = 3.74AARR153 pKa = 11.84KK154 pKa = 9.56AKK156 pKa = 9.88EE157 pKa = 3.61

Molecular weight: 18.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12965

30

1123

196.4

21.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.577 ± 0.56

1.226 ± 0.187

5.985 ± 0.25

6.656 ± 0.359

3.741 ± 0.179

7.536 ± 0.295

1.25 ± 0.202

7.042 ± 0.184

6.51 ± 0.344

7.096 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.281

5.191 ± 0.288

3.062 ± 0.229

3.926 ± 0.398

4.975 ± 0.189

7.111 ± 0.415

5.646 ± 0.292

7.034 ± 0.337

1.111 ± 0.102

3.795 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski