Sorghum bicolor (Sorghum) (Sorghum vulgare)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales;

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41381 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5XAS5|C5XAS5_SORBI Uncharacterized protein OS=Sorghum bicolor OX=4558 GN=SORBI_3002G328500 PE=4 SV=1
MM1 pKa = 7.55ASSNQVLVVIVVIAVATVLAAPAAALAADD30 pKa = 4.49LLVGGSQGWRR40 pKa = 11.84LDD42 pKa = 3.31FDD44 pKa = 4.2YY45 pKa = 11.34DD46 pKa = 3.81DD47 pKa = 3.73WVEE50 pKa = 3.99EE51 pKa = 3.91NDD53 pKa = 4.65FIVGDD58 pKa = 3.85TLVFKK63 pKa = 11.08YY64 pKa = 11.01AMGQHH69 pKa = 5.59NVVQATAASYY79 pKa = 9.41AACSQGNSLQVWSSGDD95 pKa = 3.49DD96 pKa = 2.86RR97 pKa = 11.84VTLNTSGPWWFFCGVGDD114 pKa = 4.37HH115 pKa = 6.68CQDD118 pKa = 2.99GMKK121 pKa = 10.55FNINVLPAVVLSPSSPPTRR140 pKa = 11.84DD141 pKa = 2.99QGGGDD146 pKa = 3.42ATGLAGAQGGGLAAAGLAAAAGVAVVAALLFF177 pKa = 4.41

Molecular weight: 17.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B6QKR8|A0A1B6QKR8_SORBI Uncharacterized protein (Fragment) OS=Sorghum bicolor OX=4558 GN=SORBI_3001G246300 PE=4 SV=1
MM1 pKa = 7.69ACTTLSLLDD10 pKa = 3.88HH11 pKa = 6.69LLITVLLILARR22 pKa = 11.84SIIIASVVVAPPPTPLPPPRR42 pKa = 11.84QRR44 pKa = 11.84APSLTPGPPPTRR56 pKa = 11.84TPLPRR61 pKa = 11.84PQLGAPARR69 pKa = 11.84GPILPPARR77 pKa = 11.84KK78 pKa = 8.94GVPPYY83 pKa = 10.59APPLPAPRR91 pKa = 11.84LGAPLPSSPLPPTRR105 pKa = 11.84APILPPRR112 pKa = 11.84RR113 pKa = 11.84KK114 pKa = 9.36GAPPSTPPLPTPKK127 pKa = 10.23LGAPLPSPPQPPARR141 pKa = 11.84APLLPPRR148 pKa = 11.84RR149 pKa = 11.84KK150 pKa = 10.08GPPPSTPPRR159 pKa = 11.84PAPRR163 pKa = 11.84LGVPLPSSPLPPTRR177 pKa = 11.84APVLPPRR184 pKa = 11.84RR185 pKa = 11.84KK186 pKa = 8.54GTPPSTPPRR195 pKa = 11.84PAPRR199 pKa = 11.84LGAPLPSSPLPPTRR213 pKa = 11.84APVLPPRR220 pKa = 11.84RR221 pKa = 11.84KK222 pKa = 9.4GAPPSTTPKK231 pKa = 9.38PAPRR235 pKa = 11.84LGAPFPSSPVPPTRR249 pKa = 11.84APGLPPRR256 pKa = 11.84RR257 pKa = 11.84KK258 pKa = 9.16GAPPATPPRR267 pKa = 11.84PAPRR271 pKa = 11.84LGAPLPSSPLPPTRR285 pKa = 11.84APVLPPRR292 pKa = 11.84RR293 pKa = 11.84KK294 pKa = 9.32GAPPATPPRR303 pKa = 11.84PAPRR307 pKa = 11.84LGAPLPSSPLPPTHH321 pKa = 7.04APVLPPRR328 pKa = 11.84RR329 pKa = 11.84KK330 pKa = 9.42GAPPSTPPLPTPKK343 pKa = 10.23LGAPLPSPPQPQARR357 pKa = 11.84APVLPPRR364 pKa = 11.84WKK366 pKa = 10.4GAPPSIAPQPTPRR379 pKa = 11.84LGAPLPSRR387 pKa = 11.84PQPPARR393 pKa = 11.84APLLPPRR400 pKa = 11.84QKK402 pKa = 10.54GAPPSTPPRR411 pKa = 11.84PAPRR415 pKa = 11.84LGAPLPSPILPPPARR430 pKa = 11.84ARR432 pKa = 11.84IPPPRR437 pKa = 11.84RR438 pKa = 11.84KK439 pKa = 8.63GTPPTAPRR447 pKa = 11.84FRR449 pKa = 11.84APIPSPIIPPPKK461 pKa = 9.97KK462 pKa = 10.17GIPPSTPPLPTPRR475 pKa = 11.84LGAPLPSPIIRR486 pKa = 11.84PPKK489 pKa = 10.11KK490 pKa = 9.06GTPPSIPPLPRR501 pKa = 11.84TPILPPPRR509 pKa = 11.84KK510 pKa = 9.28GAAPSSPPLRR520 pKa = 11.84PPRR523 pKa = 11.84RR524 pKa = 11.84FGAPVSSPRR533 pKa = 11.84FPPINN538 pKa = 3.43

Molecular weight: 55.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34001

7380

41381

16674116

29

5436

402.9

44.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.11 ± 0.019

1.995 ± 0.006

5.422 ± 0.009

5.901 ± 0.014

3.659 ± 0.008

7.202 ± 0.014

2.552 ± 0.006

4.364 ± 0.01

4.977 ± 0.012

9.527 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.005

3.526 ± 0.008

5.627 ± 0.013

3.563 ± 0.01

6.213 ± 0.011

8.399 ± 0.014

4.894 ± 0.007

6.766 ± 0.01

1.305 ± 0.004

2.584 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski