Streptococcus phage IPP45

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SDC7|A0A1S5SDC7_9CAUD Uncharacterized protein OS=Streptococcus phage IPP45 OX=1916184 GN=IPP45_00029 PE=4 SV=1
MM1 pKa = 7.61SYY3 pKa = 11.06DD4 pKa = 3.85LEE6 pKa = 4.31ILVKK10 pKa = 10.16IEE12 pKa = 4.0SGDD15 pKa = 3.82YY16 pKa = 10.44ICIAEE21 pKa = 4.54PKK23 pKa = 9.91YY24 pKa = 10.97SSPTYY29 pKa = 10.13NLGRR33 pKa = 11.84MFRR36 pKa = 11.84VAMNWDD42 pKa = 3.75FDD44 pKa = 3.55QDD46 pKa = 3.51TTYY49 pKa = 11.29NIADD53 pKa = 3.68VLDD56 pKa = 4.48NIQRR60 pKa = 11.84GISEE64 pKa = 4.42LEE66 pKa = 4.01RR67 pKa = 11.84YY68 pKa = 8.22PEE70 pKa = 4.56KK71 pKa = 10.66YY72 pKa = 9.5VQYY75 pKa = 10.07EE76 pKa = 3.96PEE78 pKa = 4.16NRR80 pKa = 11.84WGTVSVALEE89 pKa = 3.86VLKK92 pKa = 10.86SLKK95 pKa = 10.35EE96 pKa = 4.23CILEE100 pKa = 3.82QDD102 pKa = 3.07IDD104 pKa = 3.9TKK106 pKa = 10.44YY107 pKa = 11.29LYY109 pKa = 9.71MRR111 pKa = 11.84WW112 pKa = 3.45

Molecular weight: 13.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SD22|A0A1S5SD22_9CAUD Uncharacterized protein OS=Streptococcus phage IPP45 OX=1916184 GN=IPP45_00036 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.33FMSDD6 pKa = 3.58MKK8 pKa = 10.76FQLNSAGVSALLRR21 pKa = 11.84SSEE24 pKa = 3.92MQGILRR30 pKa = 11.84EE31 pKa = 4.04KK32 pKa = 9.96GQGIAEE38 pKa = 4.08RR39 pKa = 11.84AGEE42 pKa = 4.24GFEE45 pKa = 4.43LTVSPGQKK53 pKa = 9.27RR54 pKa = 11.84ANAKK58 pKa = 10.11ISTTDD63 pKa = 3.02IKK65 pKa = 11.66SMARR69 pKa = 11.84NKK71 pKa = 9.84KK72 pKa = 9.99HH73 pKa = 6.79NILLKK78 pKa = 10.84AMRR81 pKa = 4.66

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11982

30

2699

210.2

23.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.877 ± 0.469

0.651 ± 0.119

5.717 ± 0.252

7.536 ± 0.408

4.056 ± 0.219

6.835 ± 0.38

1.302 ± 0.169

6.969 ± 0.394

8.212 ± 0.369

8.488 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.284

5.525 ± 0.379

2.629 ± 0.258

4.014 ± 0.266

4.682 ± 0.306

6.869 ± 0.459

5.875 ± 0.489

5.984 ± 0.236

1.502 ± 0.172

3.906 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski