Cryomyces minteri

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetes incertae sedis; Cryomyces

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13291 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U0WXF3|A0A4U0WXF3_9PEZI TPR_REGION domain-containing protein OS=Cryomyces minteri OX=331657 GN=B0A49_09377 PE=4 SV=1
MM1 pKa = 7.78ADD3 pKa = 3.26EE4 pKa = 5.21FEE6 pKa = 4.79PSSASSNFSMFDD18 pKa = 3.24DD19 pKa = 3.94TTTLQSTTTAPSSLSSGQSPRR40 pKa = 11.84LMDD43 pKa = 3.86FVMPGSDD50 pKa = 3.1LTAPSTEE57 pKa = 3.99YY58 pKa = 11.39ANFRR62 pKa = 11.84SQHH65 pKa = 6.09PEE67 pKa = 3.51SLYY70 pKa = 10.61QPCQPSLIIQRR81 pKa = 11.84NGYY84 pKa = 8.23MDD86 pKa = 4.65EE87 pKa = 5.26DD88 pKa = 5.01DD89 pKa = 5.08DD90 pKa = 4.53VEE92 pKa = 4.31EE93 pKa = 4.58VVRR96 pKa = 11.84QTYY99 pKa = 10.03VNPDD103 pKa = 2.93HH104 pKa = 6.7EE105 pKa = 4.6AWIMRR110 pKa = 11.84LPSPSSSGSSSGSSEE125 pKa = 3.73GSPTVTCWNTFDD137 pKa = 3.37TLYY140 pKa = 10.78RR141 pKa = 11.84EE142 pKa = 4.32PVYY145 pKa = 11.37SNDD148 pKa = 3.55SPEE151 pKa = 3.89MLMLL155 pKa = 4.85

Molecular weight: 17.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V5NDD8|A0A4V5NDD8_9PEZI Arrestin_C domain-containing protein OS=Cryomyces minteri OX=331657 GN=B0A49_12775 PE=4 SV=1
MM1 pKa = 6.82QHH3 pKa = 6.98LPRR6 pKa = 11.84AGSTNFPFPVLSSHH20 pKa = 6.2GLAGSTCIISNQQQHH35 pKa = 5.59SEE37 pKa = 4.12RR38 pKa = 11.84GPLGPQHH45 pKa = 5.89STAPLSSHH53 pKa = 6.93TISPDD58 pKa = 3.33YY59 pKa = 10.98LDD61 pKa = 4.69TPTHH65 pKa = 5.8TATANTAGWHH75 pKa = 5.19GLRR78 pKa = 11.84TSKK81 pKa = 10.4HH82 pKa = 5.6LKK84 pKa = 9.18HH85 pKa = 6.37PHH87 pKa = 6.26SISTPSVLSHH97 pKa = 7.52PITSPHH103 pKa = 7.38RR104 pKa = 11.84PRR106 pKa = 11.84TDD108 pKa = 2.66NATPIKK114 pKa = 9.32ATPHH118 pKa = 6.63PPPPTPPNASANTTLAPPQAHH139 pKa = 7.47PANQRR144 pKa = 11.84PQTRR148 pKa = 11.84PTRR151 pKa = 11.84TTTPSRR157 pKa = 11.84PRR159 pKa = 11.84HH160 pKa = 5.38KK161 pKa = 10.97AMCKK165 pKa = 9.97YY166 pKa = 9.71YY167 pKa = 11.13AHH169 pKa = 6.41THH171 pKa = 6.11PCGHH175 pKa = 6.35TPVFAAFCAPAALVQRR191 pKa = 11.84PCGHH195 pKa = 6.12GEE197 pKa = 3.29IWHH200 pKa = 5.58SLRR203 pKa = 11.84VEE205 pKa = 4.4DD206 pKa = 5.36DD207 pKa = 3.78CPGCFAAPPASGAAAAGTKK226 pKa = 10.34GGGGGGGGARR236 pKa = 11.84RR237 pKa = 11.84PAAAKK242 pKa = 10.23RR243 pKa = 11.84GGRR246 pKa = 11.84RR247 pKa = 3.17

Molecular weight: 25.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13291

0

13291

6038804

50

6659

454.4

50.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.374 ± 0.023

1.02 ± 0.006

5.749 ± 0.016

6.357 ± 0.022

3.482 ± 0.013

7.085 ± 0.023

2.375 ± 0.008

4.431 ± 0.014

4.853 ± 0.018

8.787 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.008

3.531 ± 0.012

6.003 ± 0.025

4.01 ± 0.017

6.32 ± 0.019

8.125 ± 0.023

6.084 ± 0.016

6.282 ± 0.017

1.332 ± 0.008

2.681 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski