Amino acid dipepetide frequency for Mycobacterium phage PG1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.971AlaAla: 17.971 ± 1.281
1.294AlaCys: 1.294 ± 0.338
7.253AlaAsp: 7.253 ± 0.605
7.669AlaGlu: 7.669 ± 0.763
2.402AlaPhe: 2.402 ± 0.371
10.533AlaGly: 10.533 ± 1.381
2.31AlaHis: 2.31 ± 0.424
4.574AlaIle: 4.574 ± 0.557
3.511AlaLys: 3.511 ± 0.482
10.395AlaLeu: 10.395 ± 0.955
3.372AlaMet: 3.372 ± 0.484
3.003AlaAsn: 3.003 ± 0.366
6.699AlaPro: 6.699 ± 0.509
4.019AlaGln: 4.019 ± 0.487
8.962AlaArg: 8.962 ± 0.955
5.729AlaSer: 5.729 ± 0.589
8.085AlaThr: 8.085 ± 0.608
9.332AlaVal: 9.332 ± 0.863
2.079AlaTrp: 2.079 ± 0.338
2.91AlaTyr: 2.91 ± 0.381
0.0AlaXaa: 0.0 ± 0.0
Cys
0.739CysAla: 0.739 ± 0.197
0.046CysCys: 0.046 ± 0.049
0.924CysAsp: 0.924 ± 0.215
0.832CysGlu: 0.832 ± 0.205
0.37CysPhe: 0.37 ± 0.127
1.432CysGly: 1.432 ± 0.292
0.323CysHis: 0.323 ± 0.147
0.37CysIle: 0.37 ± 0.145
0.323CysLys: 0.323 ± 0.132
0.693CysLeu: 0.693 ± 0.201
0.231CysMet: 0.231 ± 0.102
0.323CysAsn: 0.323 ± 0.145
0.878CysPro: 0.878 ± 0.309
0.277CysGln: 0.277 ± 0.106
1.016CysArg: 1.016 ± 0.277
0.508CysSer: 0.508 ± 0.164
0.554CysThr: 0.554 ± 0.191
0.878CysVal: 0.878 ± 0.248
0.231CysTrp: 0.231 ± 0.105
0.323CysTyr: 0.323 ± 0.111
0.0CysXaa: 0.0 ± 0.0
Asp
7.161AspAla: 7.161 ± 0.591
0.97AspCys: 0.97 ± 0.262
4.851AspAsp: 4.851 ± 0.722
4.989AspGlu: 4.989 ± 0.702
1.663AspPhe: 1.663 ± 0.247
6.191AspGly: 6.191 ± 0.682
1.34AspHis: 1.34 ± 0.33
3.188AspIle: 3.188 ± 0.367
1.848AspLys: 1.848 ± 0.343
5.498AspLeu: 5.498 ± 0.544
1.063AspMet: 1.063 ± 0.307
2.079AspAsn: 2.079 ± 0.312
5.359AspPro: 5.359 ± 0.502
1.478AspGln: 1.478 ± 0.267
4.158AspArg: 4.158 ± 0.477
2.864AspSer: 2.864 ± 0.377
3.788AspThr: 3.788 ± 0.404
3.834AspVal: 3.834 ± 0.352
1.201AspTrp: 1.201 ± 0.258
1.848AspTyr: 1.848 ± 0.263
0.0AspXaa: 0.0 ± 0.0
Glu
7.438GluAla: 7.438 ± 0.964
0.693GluCys: 0.693 ± 0.227
3.557GluAsp: 3.557 ± 0.642
2.125GluGlu: 2.125 ± 0.299
2.079GluPhe: 2.079 ± 0.326
4.389GluGly: 4.389 ± 0.657
1.247GluHis: 1.247 ± 0.284
3.973GluIle: 3.973 ± 0.569
1.571GluLys: 1.571 ± 0.279
6.283GluLeu: 6.283 ± 0.589
0.924GluMet: 0.924 ± 0.204
0.97GluAsn: 0.97 ± 0.189
3.372GluPro: 3.372 ± 0.57
2.541GluGln: 2.541 ± 0.388
4.25GluArg: 4.25 ± 0.535
1.709GluSer: 1.709 ± 0.34
3.603GluThr: 3.603 ± 0.461
5.867GluVal: 5.867 ± 0.599
0.97GluTrp: 0.97 ± 0.234
1.155GluTyr: 1.155 ± 0.223
0.0GluXaa: 0.0 ± 0.0
Phe
3.049PheAla: 3.049 ± 0.38
0.277PheCys: 0.277 ± 0.107
1.663PheAsp: 1.663 ± 0.25
1.386PheGlu: 1.386 ± 0.235
0.416PhePhe: 0.416 ± 0.122
2.957PheGly: 2.957 ± 0.477
0.462PheHis: 0.462 ± 0.133
0.601PheIle: 0.601 ± 0.162
1.063PheLys: 1.063 ± 0.23
1.478PheLeu: 1.478 ± 0.25
0.277PheMet: 0.277 ± 0.113
0.878PheAsn: 0.878 ± 0.24
1.294PhePro: 1.294 ± 0.234
0.601PheGln: 0.601 ± 0.165
1.525PheArg: 1.525 ± 0.293
1.155PheSer: 1.155 ± 0.282
1.571PheThr: 1.571 ± 0.265
1.478PheVal: 1.478 ± 0.258
0.323PheTrp: 0.323 ± 0.123
0.647PheTyr: 0.647 ± 0.174
0.0PheXaa: 0.0 ± 0.0
Gly
9.563GlyAla: 9.563 ± 1.515
1.155GlyCys: 1.155 ± 0.317
5.313GlyAsp: 5.313 ± 0.595
5.174GlyGlu: 5.174 ± 0.639
2.079GlyPhe: 2.079 ± 0.341
13.259GlyGly: 13.259 ± 3.16
1.894GlyHis: 1.894 ± 0.399
4.805GlyIle: 4.805 ± 0.352
3.326GlyLys: 3.326 ± 0.451
7.9GlyLeu: 7.9 ± 0.923
2.125GlyMet: 2.125 ± 0.339
2.402GlyAsn: 2.402 ± 0.515
4.435GlyPro: 4.435 ± 0.606
3.511GlyGln: 3.511 ± 0.627
5.96GlyArg: 5.96 ± 0.599
5.22GlySer: 5.22 ± 0.495
6.791GlyThr: 6.791 ± 0.745
6.699GlyVal: 6.699 ± 0.626
1.894GlyTrp: 1.894 ± 0.402
2.633GlyTyr: 2.633 ± 0.289
0.0GlyXaa: 0.0 ± 0.0
His
1.987HisAla: 1.987 ± 0.398
0.416HisCys: 0.416 ± 0.141
1.247HisAsp: 1.247 ± 0.321
1.478HisGlu: 1.478 ± 0.269
0.462HisPhe: 0.462 ± 0.141
1.525HisGly: 1.525 ± 0.26
0.739HisHis: 0.739 ± 0.251
1.386HisIle: 1.386 ± 0.304
0.416HisLys: 0.416 ± 0.164
1.386HisLeu: 1.386 ± 0.316
0.462HisMet: 0.462 ± 0.156
0.416HisAsn: 0.416 ± 0.137
1.155HisPro: 1.155 ± 0.296
0.185HisGln: 0.185 ± 0.113
2.356HisArg: 2.356 ± 0.413
0.878HisSer: 0.878 ± 0.214
1.848HisThr: 1.848 ± 0.411
0.97HisVal: 0.97 ± 0.278
0.185HisTrp: 0.185 ± 0.092
0.601HisTyr: 0.601 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
5.729IleAla: 5.729 ± 0.515
0.554IleCys: 0.554 ± 0.164
3.788IleAsp: 3.788 ± 0.445
4.666IleGlu: 4.666 ± 0.548
0.878IlePhe: 0.878 ± 0.22
5.405IleGly: 5.405 ± 0.526
0.739IleHis: 0.739 ± 0.193
1.663IleIle: 1.663 ± 0.297
1.386IleLys: 1.386 ± 0.333
2.91IleLeu: 2.91 ± 0.402
0.832IleMet: 0.832 ± 0.215
1.478IleAsn: 1.478 ± 0.295
2.726IlePro: 2.726 ± 0.379
0.878IleGln: 0.878 ± 0.233
2.217IleArg: 2.217 ± 0.363
2.402IleSer: 2.402 ± 0.324
3.419IleThr: 3.419 ± 0.372
3.188IleVal: 3.188 ± 0.439
0.37IleTrp: 0.37 ± 0.135
0.97IleTyr: 0.97 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
4.204LysAla: 4.204 ± 0.542
0.139LysCys: 0.139 ± 0.089
1.432LysAsp: 1.432 ± 0.235
0.693LysGlu: 0.693 ± 0.266
0.554LysPhe: 0.554 ± 0.147
2.679LysGly: 2.679 ± 0.35
0.554LysHis: 0.554 ± 0.176
1.525LysIle: 1.525 ± 0.259
0.97LysLys: 0.97 ± 0.265
2.864LysLeu: 2.864 ± 0.323
0.323LysMet: 0.323 ± 0.125
0.739LysAsn: 0.739 ± 0.215
1.386LysPro: 1.386 ± 0.236
1.432LysGln: 1.432 ± 0.262
2.125LysArg: 2.125 ± 0.338
1.34LysSer: 1.34 ± 0.231
2.125LysThr: 2.125 ± 0.329
2.726LysVal: 2.726 ± 0.306
0.323LysTrp: 0.323 ± 0.112
0.647LysTyr: 0.647 ± 0.2
0.0LysXaa: 0.0 ± 0.0
Leu
11.226LeuAla: 11.226 ± 0.874
1.063LeuCys: 1.063 ± 0.249
6.283LeuAsp: 6.283 ± 0.658
3.188LeuGlu: 3.188 ± 0.321
1.848LeuPhe: 1.848 ± 0.403
7.345LeuGly: 7.345 ± 0.997
1.063LeuHis: 1.063 ± 0.224
3.973LeuIle: 3.973 ± 0.519
2.541LeuLys: 2.541 ± 0.432
5.313LeuLeu: 5.313 ± 0.524
1.571LeuMet: 1.571 ± 0.241
3.188LeuAsn: 3.188 ± 0.522
5.913LeuPro: 5.913 ± 0.476
1.571LeuGln: 1.571 ± 0.287
4.296LeuArg: 4.296 ± 0.56
4.019LeuSer: 4.019 ± 0.414
7.114LeuThr: 7.114 ± 0.608
4.897LeuVal: 4.897 ± 0.472
1.201LeuTrp: 1.201 ± 0.203
1.617LeuTyr: 1.617 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.541MetAla: 2.541 ± 0.364
0.231MetCys: 0.231 ± 0.097
0.554MetAsp: 0.554 ± 0.165
0.739MetGlu: 0.739 ± 0.164
0.785MetPhe: 0.785 ± 0.18
1.294MetGly: 1.294 ± 0.231
0.462MetHis: 0.462 ± 0.176
0.832MetIle: 0.832 ± 0.174
0.601MetLys: 0.601 ± 0.16
1.432MetLeu: 1.432 ± 0.206
0.416MetMet: 0.416 ± 0.127
0.462MetAsn: 0.462 ± 0.14
1.525MetPro: 1.525 ± 0.276
0.37MetGln: 0.37 ± 0.12
1.478MetArg: 1.478 ± 0.285
2.033MetSer: 2.033 ± 0.295
2.448MetThr: 2.448 ± 0.341
1.386MetVal: 1.386 ± 0.258
0.601MetTrp: 0.601 ± 0.177
0.508MetTyr: 0.508 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
3.465AsnAla: 3.465 ± 0.437
0.277AsnCys: 0.277 ± 0.102
1.94AsnAsp: 1.94 ± 0.318
1.247AsnGlu: 1.247 ± 0.238
0.323AsnPhe: 0.323 ± 0.097
3.372AsnGly: 3.372 ± 0.415
0.508AsnHis: 0.508 ± 0.187
1.386AsnIle: 1.386 ± 0.304
0.924AsnLys: 0.924 ± 0.238
1.802AsnLeu: 1.802 ± 0.33
0.462AsnMet: 0.462 ± 0.128
1.247AsnAsn: 1.247 ± 0.326
1.894AsnPro: 1.894 ± 0.36
0.323AsnGln: 0.323 ± 0.114
1.94AsnArg: 1.94 ± 0.343
1.709AsnSer: 1.709 ± 0.293
2.495AsnThr: 2.495 ± 0.427
1.709AsnVal: 1.709 ± 0.27
0.37AsnTrp: 0.37 ± 0.143
0.878AsnTyr: 0.878 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
7.576ProAla: 7.576 ± 0.469
0.416ProCys: 0.416 ± 0.154
4.296ProAsp: 4.296 ± 0.609
5.267ProGlu: 5.267 ± 0.738
1.34ProPhe: 1.34 ± 0.278
5.913ProGly: 5.913 ± 0.749
1.386ProHis: 1.386 ± 0.341
2.679ProIle: 2.679 ± 0.482
1.109ProLys: 1.109 ± 0.231
3.465ProLeu: 3.465 ± 0.332
1.247ProMet: 1.247 ± 0.224
1.987ProAsn: 1.987 ± 0.238
4.758ProPro: 4.758 ± 0.589
1.802ProGln: 1.802 ± 0.286
3.834ProArg: 3.834 ± 0.52
2.541ProSer: 2.541 ± 0.365
4.065ProThr: 4.065 ± 0.364
5.128ProVal: 5.128 ± 0.536
1.34ProTrp: 1.34 ± 0.253
1.109ProTyr: 1.109 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
4.065GlnAla: 4.065 ± 0.662
0.277GlnCys: 0.277 ± 0.12
1.063GlnAsp: 1.063 ± 0.262
0.785GlnGlu: 0.785 ± 0.195
1.016GlnPhe: 1.016 ± 0.179
2.171GlnGly: 2.171 ± 0.346
0.878GlnHis: 0.878 ± 0.182
2.079GlnIle: 2.079 ± 0.287
0.785GlnLys: 0.785 ± 0.204
2.448GlnLeu: 2.448 ± 0.333
0.647GlnMet: 0.647 ± 0.145
0.739GlnAsn: 0.739 ± 0.224
1.663GlnPro: 1.663 ± 0.248
1.571GlnGln: 1.571 ± 0.257
3.049GlnArg: 3.049 ± 0.442
1.756GlnSer: 1.756 ± 0.292
2.264GlnThr: 2.264 ± 0.297
2.033GlnVal: 2.033 ± 0.291
0.693GlnTrp: 0.693 ± 0.188
0.693GlnTyr: 0.693 ± 0.222
0.0GlnXaa: 0.0 ± 0.0
Arg
7.438ArgAla: 7.438 ± 0.723
0.647ArgCys: 0.647 ± 0.193
4.435ArgAsp: 4.435 ± 0.451
4.989ArgGlu: 4.989 ± 0.667
1.571ArgPhe: 1.571 ± 0.288
4.389ArgGly: 4.389 ± 0.559
1.617ArgHis: 1.617 ± 0.356
2.402ArgIle: 2.402 ± 0.334
1.802ArgLys: 1.802 ± 0.312
5.867ArgLeu: 5.867 ± 0.513
1.571ArgMet: 1.571 ± 0.348
2.356ArgAsn: 2.356 ± 0.413
4.158ArgPro: 4.158 ± 0.513
2.772ArgGln: 2.772 ± 0.422
6.791ArgArg: 6.791 ± 0.838
2.772ArgSer: 2.772 ± 0.291
4.065ArgThr: 4.065 ± 0.494
5.498ArgVal: 5.498 ± 0.69
1.894ArgTrp: 1.894 ± 0.334
2.264ArgTyr: 2.264 ± 0.451
0.0ArgXaa: 0.0 ± 0.0
Ser
5.128SerAla: 5.128 ± 0.51
0.647SerCys: 0.647 ± 0.176
3.419SerAsp: 3.419 ± 0.353
2.125SerGlu: 2.125 ± 0.337
0.693SerPhe: 0.693 ± 0.188
6.098SerGly: 6.098 ± 0.773
0.878SerHis: 0.878 ± 0.197
1.756SerIle: 1.756 ± 0.329
1.201SerLys: 1.201 ± 0.25
4.158SerLeu: 4.158 ± 0.707
1.247SerMet: 1.247 ± 0.279
1.109SerAsn: 1.109 ± 0.241
3.049SerPro: 3.049 ± 0.426
2.033SerGln: 2.033 ± 0.358
3.465SerArg: 3.465 ± 0.383
2.448SerSer: 2.448 ± 0.434
3.881SerThr: 3.881 ± 0.522
3.788SerVal: 3.788 ± 0.407
1.016SerTrp: 1.016 ± 0.197
1.525SerTyr: 1.525 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
8.962ThrAla: 8.962 ± 0.522
0.462ThrCys: 0.462 ± 0.151
5.267ThrAsp: 5.267 ± 0.527
3.419ThrGlu: 3.419 ± 0.415
1.663ThrPhe: 1.663 ± 0.255
6.883ThrGly: 6.883 ± 0.57
1.063ThrHis: 1.063 ± 0.263
3.788ThrIle: 3.788 ± 0.481
2.495ThrLys: 2.495 ± 0.281
5.313ThrLeu: 5.313 ± 0.542
1.663ThrMet: 1.663 ± 0.254
1.94ThrAsn: 1.94 ± 0.414
5.082ThrPro: 5.082 ± 0.629
1.802ThrGln: 1.802 ± 0.302
3.696ThrArg: 3.696 ± 0.427
4.065ThrSer: 4.065 ± 0.36
3.973ThrThr: 3.973 ± 0.585
6.052ThrVal: 6.052 ± 0.532
1.294ThrTrp: 1.294 ± 0.255
1.663ThrTyr: 1.663 ± 0.304
0.0ThrXaa: 0.0 ± 0.0
Val
8.316ValAla: 8.316 ± 0.489
0.97ValCys: 0.97 ± 0.224
4.527ValAsp: 4.527 ± 0.525
5.913ValGlu: 5.913 ± 0.715
1.571ValPhe: 1.571 ± 0.228
7.114ValGly: 7.114 ± 0.623
1.663ValHis: 1.663 ± 0.357
3.372ValIle: 3.372 ± 0.349
1.94ValLys: 1.94 ± 0.297
6.468ValLeu: 6.468 ± 0.454
1.386ValMet: 1.386 ± 0.274
2.033ValAsn: 2.033 ± 0.288
3.973ValPro: 3.973 ± 0.393
1.894ValGln: 1.894 ± 0.279
4.712ValArg: 4.712 ± 0.539
4.112ValSer: 4.112 ± 0.413
5.267ValThr: 5.267 ± 0.499
5.913ValVal: 5.913 ± 0.675
1.894ValTrp: 1.894 ± 0.299
1.987ValTyr: 1.987 ± 0.38
0.0ValXaa: 0.0 ± 0.0
Trp
2.31TrpAla: 2.31 ± 0.388
0.416TrpCys: 0.416 ± 0.136
1.386TrpAsp: 1.386 ± 0.288
0.554TrpGlu: 0.554 ± 0.16
0.785TrpPhe: 0.785 ± 0.269
1.063TrpGly: 1.063 ± 0.217
0.508TrpHis: 0.508 ± 0.19
0.878TrpIle: 0.878 ± 0.165
0.37TrpLys: 0.37 ± 0.142
1.525TrpLeu: 1.525 ± 0.349
0.508TrpMet: 0.508 ± 0.152
0.277TrpAsn: 0.277 ± 0.101
0.878TrpPro: 0.878 ± 0.194
0.693TrpGln: 0.693 ± 0.19
1.34TrpArg: 1.34 ± 0.207
1.386TrpSer: 1.386 ± 0.28
1.478TrpThr: 1.478 ± 0.282
1.432TrpVal: 1.432 ± 0.27
0.462TrpTrp: 0.462 ± 0.148
0.37TrpTyr: 0.37 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.234TyrAla: 3.234 ± 0.325
0.323TyrCys: 0.323 ± 0.122
2.171TyrAsp: 2.171 ± 0.265
1.478TyrGlu: 1.478 ± 0.295
0.601TyrPhe: 0.601 ± 0.178
2.171TyrGly: 2.171 ± 0.372
0.462TyrHis: 0.462 ± 0.14
0.97TyrIle: 0.97 ± 0.2
0.601TyrLys: 0.601 ± 0.161
2.033TyrLeu: 2.033 ± 0.351
0.323TyrMet: 0.323 ± 0.132
0.647TyrAsn: 0.647 ± 0.148
1.063TyrPro: 1.063 ± 0.243
0.924TyrGln: 0.924 ± 0.191
2.125TyrArg: 2.125 ± 0.374
1.016TyrSer: 1.016 ± 0.176
1.709TyrThr: 1.709 ± 0.264
2.217TyrVal: 2.217 ± 0.302
0.277TyrTrp: 0.277 ± 0.114
0.601TyrTyr: 0.601 ± 0.179
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (21647 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski