Halobacillus sp. BAB-2008

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halobacillus; unclassified Halobacillus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3935 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L5N8S3|L5N8S3_9BACI Competence protein ComFC OS=Halobacillus sp. BAB-2008 OX=1246484 GN=D479_07996 PE=4 SV=1
MM1 pKa = 7.66LSLNVPDD8 pKa = 6.56LITCQAVITGTLTCAEE24 pKa = 4.51DD25 pKa = 3.96TPVANAEE32 pKa = 4.07VSFLDD37 pKa = 4.07FPEE40 pKa = 5.65DD41 pKa = 3.2NVTYY45 pKa = 10.44NPNPAITDD53 pKa = 3.55ANGNFSTTVTVNPNTEE69 pKa = 4.12LLSLVVNASSVVFGEE84 pKa = 4.33VQQGSAGTQIICSPDD99 pKa = 3.56PPPPPVEE106 pKa = 4.47CPCKK110 pKa = 10.22FRR112 pKa = 11.84LGVARR117 pKa = 11.84NRR119 pKa = 11.84ATATATVVDD128 pKa = 4.17NGAPFDD134 pKa = 3.69LTGIINITAVQCFTASTMCNPNVDD158 pKa = 3.56NFNITFNAGGTTINFVQGRR177 pKa = 11.84RR178 pKa = 11.84IEE180 pKa = 4.18IGCEE184 pKa = 3.72GNNVARR190 pKa = 11.84IRR192 pKa = 11.84GTAIGRR198 pKa = 11.84GNLYY202 pKa = 10.72SGLFDD207 pKa = 3.82VNIEE211 pKa = 4.37VIVDD215 pKa = 3.63EE216 pKa = 4.85NNVGTWTIDD225 pKa = 3.0AFDD228 pKa = 4.74DD229 pKa = 3.98MNHH232 pKa = 5.9SFSTVFTALMNPITSIGEE250 pKa = 3.9CDD252 pKa = 3.48VQFF255 pKa = 4.29

Molecular weight: 27.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L5NEW9|L5NEW9_9BACI Riboflavin biosynthesis protein RibD OS=Halobacillus sp. BAB-2008 OX=1246484 GN=D479_00035 PE=3 SV=1
MM1 pKa = 7.43NEE3 pKa = 4.16AIEE6 pKa = 4.41KK7 pKa = 9.49IKK9 pKa = 10.46RR10 pKa = 11.84GRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.48RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84WIIVNGLLALFAVVLSGTMLVMGNTVYY45 pKa = 10.87SIPEE49 pKa = 3.92VIAALSGDD57 pKa = 3.62ASGGADD63 pKa = 3.15FAVNTIRR70 pKa = 11.84LPRR73 pKa = 11.84MVAGLLAGIAFGIAGNTFQTMLRR96 pKa = 11.84NPLANPDD103 pKa = 3.27ILGITTGSSVAAVYY117 pKa = 10.28CILILQTSEE126 pKa = 3.86GTASAAAIIGGLTAVVVMYY145 pKa = 10.59ALSRR149 pKa = 11.84GRR151 pKa = 11.84TFSIGRR157 pKa = 11.84LILIGIGIQAMLSSAISYY175 pKa = 10.92LLMKK179 pKa = 10.36GGRR182 pKa = 11.84QDD184 pKa = 4.59LPTAIQWLSGSLNGSQMDD202 pKa = 3.71KK203 pKa = 10.78LGPLAITVLICVPVVVVLGKK223 pKa = 9.92HH224 pKa = 6.09LSILEE229 pKa = 4.39LGEE232 pKa = 3.73QSAASLGLNTDD243 pKa = 3.16RR244 pKa = 11.84TRR246 pKa = 11.84ILLIISSVAMLALATSVTGPISFVAFLAGPIAKK279 pKa = 9.67RR280 pKa = 11.84LVGVGYY286 pKa = 10.79SNVIPAGLVGVNLVLGADD304 pKa = 5.04LIGQFAFDD312 pKa = 3.12VRR314 pKa = 11.84FPVGIITGILGAPFLLYY331 pKa = 10.96LLIRR335 pKa = 11.84MNRR338 pKa = 11.84KK339 pKa = 9.38GSLSS343 pKa = 3.31

Molecular weight: 35.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3935

0

3935

1089697

26

1540

276.9

31.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.504 ± 0.039

0.65 ± 0.012

5.57 ± 0.039

7.982 ± 0.056

4.408 ± 0.034

7.35 ± 0.04

2.233 ± 0.02

6.813 ± 0.045

6.062 ± 0.04

9.607 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.983 ± 0.021

3.554 ± 0.022

3.801 ± 0.02

3.705 ± 0.025

4.596 ± 0.034

5.896 ± 0.031

5.398 ± 0.025

7.32 ± 0.031

1.131 ± 0.016

3.437 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski