Cleome golden mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5A3L0|F5A3L0_9GEMI Transcriptional activator protein OS=Cleome golden mosaic virus OX=858517 PE=3 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84GSSSSNPPCIKK13 pKa = 10.07AQHH16 pKa = 6.22KK17 pKa = 8.66AAKK20 pKa = 9.42RR21 pKa = 11.84RR22 pKa = 11.84AIRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84IDD30 pKa = 3.52LTCGCTIYY38 pKa = 10.4IHH40 pKa = 6.57LNCHH44 pKa = 5.55SNEE47 pKa = 3.86FTHH50 pKa = 7.07RR51 pKa = 11.84GTSSAVPQAEE61 pKa = 3.97NGVYY65 pKa = 8.4TWEE68 pKa = 4.29ISNPLYY74 pKa = 10.67FRR76 pKa = 11.84IHH78 pKa = 6.45DD79 pKa = 4.01VEE81 pKa = 5.06DD82 pKa = 3.64VLHH85 pKa = 6.86SRR87 pKa = 11.84DD88 pKa = 3.41EE89 pKa = 4.91DD90 pKa = 3.4IPRR93 pKa = 11.84PDD95 pKa = 3.09TVQPQRR101 pKa = 11.84EE102 pKa = 3.99EE103 pKa = 4.85SIGSPHH109 pKa = 7.09GLSQLPSLDD118 pKa = 3.73SLGDD122 pKa = 3.91SFWADD127 pKa = 3.23LFKK130 pKa = 11.23

Molecular weight: 14.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5A3L0|F5A3L0_9GEMI Transcriptional activator protein OS=Cleome golden mosaic virus OX=858517 PE=3 SV=1
MM1 pKa = 7.62NSRR4 pKa = 11.84TGEE7 pKa = 3.95RR8 pKa = 11.84HH9 pKa = 4.06QLCLRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84MACIHH21 pKa = 6.17GRR23 pKa = 11.84YY24 pKa = 9.17QIPCISGYY32 pKa = 5.19TTWRR36 pKa = 11.84TSCTAEE42 pKa = 3.53TRR44 pKa = 11.84IYY46 pKa = 10.27HH47 pKa = 4.19VQIRR51 pKa = 11.84FNHH54 pKa = 4.93SVRR57 pKa = 11.84RR58 pKa = 11.84ALALHH63 pKa = 5.73MAFLNFQVWTVSVTASGQTYY83 pKa = 10.58LNRR86 pKa = 11.84FKK88 pKa = 10.99HH89 pKa = 5.51LVLMYY94 pKa = 10.27LDD96 pKa = 3.56QLGVISLNNVIRR108 pKa = 11.84AVSFATDD115 pKa = 3.24RR116 pKa = 11.84SYY118 pKa = 11.75VSHH121 pKa = 5.26VHH123 pKa = 6.04EE124 pKa = 4.01NHH126 pKa = 6.19SIKK129 pKa = 10.63FKK131 pKa = 10.76FYY133 pKa = 10.95

Molecular weight: 15.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

514

130

251

171.3

19.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.031 ± 0.235

2.529 ± 0.335

4.28 ± 1.063

3.502 ± 0.618

3.891 ± 0.486

5.253 ± 0.581

4.669 ± 0.995

5.253 ± 0.794

4.28 ± 1.091

6.226 ± 0.97

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.564

5.837 ± 0.849

4.669 ± 1.386

3.502 ± 0.479

9.728 ± 0.283

8.171 ± 1.227

5.837 ± 0.605

7.004 ± 0.993

1.751 ± 0.152

5.058 ± 0.949
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski