Streptococcus phage phiJH1301-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9I747|A0A1X9I747_9CAUD Endopeptidase OS=Streptococcus phage phiJH1301-2 OX=1860194 PE=4 SV=1
MM1 pKa = 7.86PLFSVQGLDD10 pKa = 4.57NIVLSAGHH18 pKa = 6.48GSSFYY23 pKa = 10.93DD24 pKa = 3.84EE25 pKa = 4.43YY26 pKa = 11.39EE27 pKa = 3.82SWEE30 pKa = 4.07VFTNKK35 pKa = 10.37DD36 pKa = 3.25YY37 pKa = 11.31LYY39 pKa = 11.04DD40 pKa = 3.41VATWITSHH48 pKa = 6.93SMEE51 pKa = 4.58PVYY54 pKa = 10.7QDD56 pKa = 3.8GEE58 pKa = 4.26VALIRR63 pKa = 11.84EE64 pKa = 4.47GGFDD68 pKa = 3.79YY69 pKa = 11.18DD70 pKa = 3.72GAVYY74 pKa = 10.0AVAWNEE80 pKa = 3.35QLYY83 pKa = 9.88IKK85 pKa = 10.3KK86 pKa = 10.44VYY88 pKa = 10.63LEE90 pKa = 4.17NNGFRR95 pKa = 11.84LVSINRR101 pKa = 3.59

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9I6D2|A0A1X9I6D2_9CAUD Uncharacterized protein OS=Streptococcus phage phiJH1301-2 OX=1860194 PE=4 SV=1
MM1 pKa = 7.75PRR3 pKa = 11.84RR4 pKa = 11.84PNTPCKK10 pKa = 10.42QNGCPNLVPYY20 pKa = 8.51GQKK23 pKa = 10.57YY24 pKa = 9.81CDD26 pKa = 3.33NHH28 pKa = 5.77KK29 pKa = 11.09ANYY32 pKa = 9.49QLDD35 pKa = 4.17TKK37 pKa = 8.57STKK40 pKa = 10.53AKK42 pKa = 10.29GYY44 pKa = 8.38NAQWNKK50 pKa = 10.15ARR52 pKa = 11.84LRR54 pKa = 11.84YY55 pKa = 9.99LKK57 pKa = 9.81VHH59 pKa = 6.46PLCVQCKK66 pKa = 8.68VKK68 pKa = 10.55GRR70 pKa = 11.84LTKK73 pKa = 10.1ATVVDD78 pKa = 5.28HH79 pKa = 6.23ITPHH83 pKa = 6.97RR84 pKa = 11.84GDD86 pKa = 5.06QEE88 pKa = 5.01LFWNQSNWQALCKK101 pKa = 10.09SCHH104 pKa = 5.64DD105 pKa = 4.22RR106 pKa = 11.84KK107 pKa = 8.93TKK109 pKa = 8.13TTDD112 pKa = 2.58RR113 pKa = 11.84YY114 pKa = 10.6VEE116 pKa = 3.73YY117 pKa = 9.41TYY119 pKa = 11.18RR120 pKa = 11.84FF121 pKa = 3.55

Molecular weight: 14.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

19713

41

1515

259.4

29.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.194 ± 0.436

0.949 ± 0.137

5.747 ± 0.232

7.695 ± 0.333

4.124 ± 0.178

6.209 ± 0.232

1.882 ± 0.113

6.94 ± 0.225

7.655 ± 0.286

9.217 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.364 ± 0.144

5.103 ± 0.21

2.912 ± 0.144

4.063 ± 0.183

4.429 ± 0.231

6.442 ± 0.282

6.209 ± 0.331

6.27 ± 0.189

1.39 ± 0.082

4.205 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski