Planoprotostelium fungivorum

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Evosea; Variosea; Cavosteliida; Cavosteliaceae; Planoprotostelium

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P6N3J0|A0A2P6N3J0_9EUKA Filamentous hemagglutinin family outer membrane protein OS=Planoprotostelium fungivorum OX=1890364 GN=PROFUN_13585 PE=4 SV=1
MM1 pKa = 7.37AAAVSSSAVVKK12 pKa = 10.52VVGTEE17 pKa = 3.82EE18 pKa = 4.46EE19 pKa = 3.92ITVNVNDD26 pKa = 3.66KK27 pKa = 11.08GFYY30 pKa = 10.63DD31 pKa = 3.64EE32 pKa = 6.15VEE34 pKa = 4.15IEE36 pKa = 4.31DD37 pKa = 4.75LEE39 pKa = 4.27FDD41 pKa = 3.74EE42 pKa = 6.52DD43 pKa = 4.79SEE45 pKa = 4.68TFYY48 pKa = 11.31YY49 pKa = 9.87PCPCGDD55 pKa = 3.53RR56 pKa = 11.84FQVTLEE62 pKa = 3.91EE63 pKa = 4.28LKK65 pKa = 10.94SGEE68 pKa = 4.17EE69 pKa = 3.76IARR72 pKa = 11.84CPSCSLLIRR81 pKa = 11.84IIYY84 pKa = 9.93SPEE87 pKa = 3.81DD88 pKa = 3.42LEE90 pKa = 4.5EE91 pKa = 5.22VEE93 pKa = 6.09DD94 pKa = 4.02EE95 pKa = 4.32

Molecular weight: 10.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P6MML6|A0A2P6MML6_9EUKA Uncharacterized protein OS=Planoprotostelium fungivorum OX=1890364 GN=PROFUN_16237 PE=4 SV=1
AA1 pKa = 6.98TAPRR5 pKa = 11.84RR6 pKa = 11.84NSRR9 pKa = 11.84KK10 pKa = 9.02LQHH13 pKa = 6.45LRR15 pKa = 11.84VAHH18 pKa = 5.15ATGRR22 pKa = 11.84TAMSQQRR29 pKa = 11.84TTLAGMVLRR38 pKa = 11.84TACFTQRR45 pKa = 11.84TTFTGGRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84GMLSLAVSIWFNN66 pKa = 3.33

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16856

0

16856

8545775

39

9664

507.0

56.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.257 ± 0.017

1.589 ± 0.01

5.343 ± 0.014

6.638 ± 0.04

3.906 ± 0.012

5.806 ± 0.021

2.458 ± 0.01

5.716 ± 0.015

5.796 ± 0.029

9.098 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.008

4.646 ± 0.016

4.797 ± 0.016

4.192 ± 0.012

5.649 ± 0.02

8.654 ± 0.024

6.442 ± 0.025

6.263 ± 0.015

1.291 ± 0.007

3.02 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski