Salmonella phage epsilon15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; Salmonella virus Epsilon15

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q858G6|Q858G6_BPE15 Uncharacterized protein OS=Salmonella phage epsilon15 OX=215158 PE=4 SV=1
MM1 pKa = 7.04KK2 pKa = 9.48TVNMKK7 pKa = 9.57TGTDD11 pKa = 3.47SFVGEE16 pKa = 4.34DD17 pKa = 4.08GKK19 pKa = 10.18PEE21 pKa = 4.15TKK23 pKa = 10.27DD24 pKa = 3.14QYY26 pKa = 11.07PWGLRR31 pKa = 11.84ITLDD35 pKa = 3.44NEE37 pKa = 4.1SLQRR41 pKa = 11.84LGLNAKK47 pKa = 9.35SLPAVGDD54 pKa = 4.16SVSVMAMANVCSVSTRR70 pKa = 11.84TTDD73 pKa = 2.94HH74 pKa = 6.86GEE76 pKa = 3.94DD77 pKa = 3.75NYY79 pKa = 10.97VEE81 pKa = 4.35LQITDD86 pKa = 3.17IGLAPQKK93 pKa = 10.57RR94 pKa = 11.84DD95 pKa = 3.42DD96 pKa = 3.98AKK98 pKa = 10.15EE99 pKa = 4.01LKK101 pKa = 10.45DD102 pKa = 3.47AFYY105 pKa = 10.6PDD107 pKa = 4.42GEE109 pKa = 4.92DD110 pKa = 4.08DD111 pKa = 3.58

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q858F2|Q858F2_BPE15 Uncharacterized protein OS=Salmonella phage epsilon15 OX=215158 PE=4 SV=2
MM1 pKa = 6.55TTHH4 pKa = 6.96EE5 pKa = 4.9LLLLIANAVICSAIAIRR22 pKa = 11.84VGTFRR27 pKa = 11.84RR28 pKa = 11.84NGSQHH33 pKa = 6.14RR34 pKa = 11.84RR35 pKa = 11.84WGGWIAYY42 pKa = 7.61FLIVASASIPVRR54 pKa = 11.84AAYY57 pKa = 9.5AIWYY61 pKa = 5.87HH62 pKa = 5.56TPMAADD68 pKa = 3.98LSEE71 pKa = 4.39VIINAVMLAAVLKK84 pKa = 9.37TRR86 pKa = 11.84GNVVQMFKK94 pKa = 10.43ISRR97 pKa = 11.84SQHH100 pKa = 3.2GHH102 pKa = 4.83

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12421

35

1070

243.5

26.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.426 ± 0.659

0.958 ± 0.147

6.143 ± 0.222

5.781 ± 0.426

3.615 ± 0.225

7.399 ± 0.374

1.691 ± 0.183

5.684 ± 0.446

4.983 ± 0.369

7.672 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.249

4.621 ± 0.464

4.106 ± 0.252

4.766 ± 0.491

5.668 ± 0.295

6.658 ± 0.337

5.917 ± 0.332

6.457 ± 0.304

1.522 ± 0.177

3.164 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski