Pectobacterium phage PP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Wanjuvirus; Pectobacterium virus PP2

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5P529|A0A1W5P529_9CAUD Uncharacterized protein OS=Pectobacterium phage PP2 OX=1897743 GN=PP2_047 PE=4 SV=1
MM1 pKa = 7.17ITSQAPFLSALQGAKK16 pKa = 10.24LRR18 pKa = 11.84TLAVNLQNYY27 pKa = 9.36ADD29 pKa = 4.32NNPGAKK35 pKa = 9.28PAVYY39 pKa = 10.0QFWQSQKK46 pKa = 8.9TLASADD52 pKa = 3.44SGIPSDD58 pKa = 3.53GTYY61 pKa = 10.21IPGVKK66 pKa = 9.88DD67 pKa = 4.62LRR69 pKa = 11.84PTCYY73 pKa = 9.86KK74 pKa = 10.44VLSHH78 pKa = 6.35YY79 pKa = 9.66RR80 pKa = 11.84TFPTSDD86 pKa = 2.49ITEE89 pKa = 4.11YY90 pKa = 11.21TDD92 pKa = 3.57FCDD95 pKa = 5.49FFSAANAPVVEE106 pKa = 4.25EE107 pKa = 4.83LVFSLNLPEE116 pKa = 4.35TKK118 pKa = 9.92EE119 pKa = 4.18VAQGATLTLQGAVTGGVSPYY139 pKa = 9.91TYY141 pKa = 11.14AWLKK145 pKa = 10.82DD146 pKa = 3.48DD147 pKa = 3.77VVIEE151 pKa = 4.29GRR153 pKa = 11.84VSASFTKK160 pKa = 10.9SNFDD164 pKa = 3.27VSDD167 pKa = 3.14EE168 pKa = 4.21GKK170 pKa = 10.69YY171 pKa = 10.31VLQVTDD177 pKa = 3.92AEE179 pKa = 4.67STVILSQACGVSLAVV194 pKa = 3.76

Molecular weight: 20.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5P4Y9|A0A1W5P4Y9_9CAUD Uncharacterized protein OS=Pectobacterium phage PP2 OX=1897743 GN=PP2_003 PE=4 SV=1
MM1 pKa = 6.6NQVVFVCDD9 pKa = 3.46GTTKK13 pKa = 9.8TRR15 pKa = 11.84NVQLTAIRR23 pKa = 11.84DD24 pKa = 3.75AVSVEE29 pKa = 3.58KK30 pKa = 10.72FEE32 pKa = 4.38RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NVTAEE40 pKa = 3.85VHH42 pKa = 5.39QKK44 pKa = 10.78ALAALRR50 pKa = 11.84SEE52 pKa = 4.74YY53 pKa = 10.51ARR55 pKa = 11.84KK56 pKa = 9.02AAAVGMSLVGYY67 pKa = 7.4CARR70 pKa = 11.84FNVRR74 pKa = 11.84LPCC77 pKa = 4.93

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

12943

56

1268

275.4

30.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.928 ± 0.554

0.973 ± 0.129

6.011 ± 0.206

6.026 ± 0.306

3.662 ± 0.209

7.657 ± 0.247

2.001 ± 0.203

4.435 ± 0.238

5.377 ± 0.315

8.445 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.913 ± 0.17

4.072 ± 0.24

3.956 ± 0.202

4.373 ± 0.253

5.686 ± 0.223

6.297 ± 0.306

6.034 ± 0.368

7.17 ± 0.331

1.337 ± 0.123

3.647 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski