Aeromonas phage 85AhydR10PP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1PEL9|A0A2S1PEL9_9CAUD Uncharacterized protein OS=Aeromonas phage 85AhydR10PP OX=2163964 PE=4 SV=1
MM1 pKa = 7.41NKK3 pKa = 9.46QVIAGVTIAQGEE15 pKa = 4.55VSCGVKK21 pKa = 10.39SPAYY25 pKa = 10.05DD26 pKa = 4.39PISPEE31 pKa = 3.7VPTFGEE37 pKa = 4.64YY38 pKa = 9.3WSHH41 pKa = 5.31TAMNGLPEE49 pKa = 4.22MTWDD53 pKa = 3.51AFQARR58 pKa = 11.84MMNQYY63 pKa = 10.91GLTILYY69 pKa = 10.27RR70 pKa = 11.84SMADD74 pKa = 3.4DD75 pKa = 5.23PEE77 pKa = 4.38VDD79 pKa = 3.28WEE81 pKa = 4.36AEE83 pKa = 3.98LEE85 pKa = 4.18IEE87 pKa = 4.29SWEE90 pKa = 3.96PQNPFVSDD98 pKa = 3.28KK99 pKa = 11.54AFLLVLGEE107 pKa = 4.84DD108 pKa = 3.53EE109 pKa = 5.73DD110 pKa = 5.54GPFTVWGLPQQ120 pKa = 3.85

Molecular weight: 13.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1PEN4|A0A2S1PEN4_9CAUD Uncharacterized protein OS=Aeromonas phage 85AhydR10PP OX=2163964 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 5.27KK3 pKa = 10.56LIVGRR8 pKa = 11.84GFGPQIAQRR17 pKa = 11.84AAQALKK23 pKa = 10.7DD24 pKa = 3.85GVRR27 pKa = 11.84RR28 pKa = 11.84PIMASKK34 pKa = 10.77KK35 pKa = 6.42EE36 pKa = 3.9WKK38 pKa = 8.61MVNVGYY44 pKa = 9.79RR45 pKa = 11.84HH46 pKa = 6.42RR47 pKa = 11.84LLLRR51 pKa = 11.84GDD53 pKa = 4.17GKK55 pKa = 11.14AEE57 pKa = 4.01LMTHH61 pKa = 6.02EE62 pKa = 5.64SILKK66 pKa = 8.72YY67 pKa = 10.36LSRR70 pKa = 11.84RR71 pKa = 11.84AGNCKK76 pKa = 10.1

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14033

52

843

175.4

19.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.34 ± 0.473

1.618 ± 0.155

5.516 ± 0.207

6.207 ± 0.325

3.064 ± 0.142

7.703 ± 0.311

2.323 ± 0.186

4.981 ± 0.159

6.071 ± 0.297

8.466 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.278 ± 0.207

3.356 ± 0.274

4.34 ± 0.248

3.649 ± 0.176

5.936 ± 0.311

5.829 ± 0.226

6.257 ± 0.266

7.048 ± 0.302

1.639 ± 0.122

2.38 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski